Citation: JIANG Yalan, HUANG Fang, WU Fuhai, WU Huiqin, HUANG Xiaolan, DENG Xin. Simultaneous determination of 16 flavonoids in the ginkgo dietary supplement tea by high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(10): 1032-1039. doi: 10.3724/SP.J.1123.2015.06013 shu

Simultaneous determination of 16 flavonoids in the ginkgo dietary supplement tea by high performance liquid chromatography-tandem mass spectrometry

JIANG Yalan ^1,2 ,
HUANG Fang ³ ,
WU Fuhai ^2,, ,
WU Huiqin ³ ,
HUANG Xiaolan ³ ,
DENG Xin ³

1.
1. College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China;

Corresponding author: WU Fuhai,
Received Date: 8 June 2015

Fund Project: 广东省科技成果转化启动项目(2011A060903001). (2011A060903001)

A method for the determination of 16 functional components of ginkgo dietary supplement tea such as catechin, vitexin, puerarin, isoflavoues aglycone, silymarin, quercetin, luteolin, apigenin, naringenin, hesperitin dihydrochalcone, kaempferol, hesperitin, isorhamnetin, baicalein, nobiletin and tangeretin by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was proposed. The conditions of chromatography and mass spectrometry were optimized. The 16 flavonoids were separated on a C₁₈ chromatographic column with acetonitrile and water (additional 0.1% formic acid) as mobile phases under gradient elution at a flow rate of 0.25 mL/min. The determination was conducted by tandem mass spectrometry in positive ESI mode under multiple reaction monitoring (MRM) mode. Good linearities for all the compounds, with correlation coefficients over 0.996, were acquired. The recoveries were in the range of 70.9% to 100.0% (n=6), while the relative standard deviations (RSDs) were less than 10%. The results showed that the nine flavonoids, which were kaempferol, quercetin, hesperitin, vitexin, luteolin, catechin, apigenin, naringenin and isorhamnetin, were higher in contents among the 16 flavonoids in real samples, and they constituted up to 99.6% of the total flavonoids. The contents of these nine flavonoids can be considered as the quality control index of the ginkgo dietary supplement tea. The method proved to be rapid, selective, sensitive and stable, and it can be applied to control the quality of the ginkgo dietary supplement tea.
- liquid chromatography-tandem mass spectrometry (LC-MS/MS),
- flavonoids,
- ginkgo dietary supplement tea
1. [1]
  [1] Zhou B, Wang L, Li W, et al. Chinese Journal of Analytical Chemistry (周蓓, 王琳, 李伟, 等. 分析化学), 2008, 36(4): 494
2. [2]
  [2] Wohlmuth H, Savage K, Dowell A, et al. Phytomedicine, 2014, 21: 912
3. [3]
  [3] Chen Z P, Sun J, Chen H X, et al. Fitoterapia, 2010, 81: 1045
4. [4]
  [4] Chi Y M, Li Y, Zhang Y, et al. Chinese Journal of Chromatography (池玉梅, 李瑶, 张瑜, 等. 色谱), 2013, 31(9): 838
5. [5]
  [5] Sun Y N, Li T, Ma C. Chinese Journal of Chromatography (孙亚男, 李彤, 马辰. 色谱), 2013, 31(5): 447
6. [6]
  [6] Zheng H S, Li J P, Han W, et al. Chinese Traditional Patent Medicine (郑杭生, 李计萍, 韩炜, 等. 中成药), 2008, 30(9): 1364
7. [7]
  [7] Xiong D M, Deng Z Y, Liu R, et al. Food Science (熊冬梅, 邓泽元, 刘蓉, 等. 食品科学), 2009, 30(22): 256
8. [8]
  [8] Zeng L, Zeng F J, Wang W, et al. Food Research and Development (曾里, 曾凡骏, 王威, 等. 食品研究与开发), 2008, 29(6): 85
9. [9]
  [9] He C M, Cheng Z H, Chen D F. Chinese Journal of Natural Medicines (何常明, 程志红, 陈道峰. 中国天然药物), 2013, 11(6): 690
10. [10]
  [10] Liu M Y, Yao Z H, Zhang Y, et al. Journal of Instrumental Analysis (刘明艳, 姚志红, 张依, 等. 分析测试学报), 2011, 30(9): 1006
11. [11]
  [11] Lin Z Y, Yang R F, Tang Y N. China Pharmacist (林志燕, 杨荣富, 唐跃年. 中国药师), 2014, 17(8): 1292
12. [12]
  [12] Wang G Y. Detection Method of Health Food Ingredients. Beijing: China Light Industry Press (王光亚. 保健食品功效成分检测方法. 北京: 中国轻工业出版社), 2002: 29
13. [13]
  [13] Zhou D Y, Xu Q, Xue X Y, et al. Chinese Journal of Analytical Chemistry (周大勇, 徐青, 薛兴亚, 等. 分析化学), 2006, 34(9): 31
14. [14]
  [14] Xiang P, Shen M, Zhuo X Y. Journal of Instrumental Analysis (向平, 沈敏, 卓先义. 分析测试学报), 2009, 28(6): 753
15. [15]
  [15] Zhang Y, Yao Z H, Qin Z F, et al. Journal of Jinan University: Natural Science & Medicine Edition (张依, 姚志红, 秦子飞, 等. 暨南大学学报: 自然科学与医学版), 2012, 33(3): 305
1. [1]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
2. [2]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
3. [3]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
4. [4]
  Yanglin Jiang , Mingqing Chen , Min Liang , Yige Yao , Yan Zhang , Peng Wang , Jianping Zhang . Experimental and Theoretical Investigations of Solvent Polarity Effect on ESIPT Mechanism in 4′-N,N-diethylamino-3-hydroxybenzoflavone. Acta Physico-Chimica Sinica, 2025, 41(2): 100012-. doi: 10.3866/PKU.WHXB202309027
5. [5]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
6. [6]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
7. [7]
  Yutong Dong , Huiling Xu , Yucheng Zhao , Zexin Zhang , Ying Wang . The Hidden World of Surface Tension and Droplets. University Chemistry, 2024, 39(6): 357-365. doi: 10.3866/PKU.DXHX202312022
8. [8]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
9. [9]
  Hao Wu , Zhen Liu , Dachang Bai . ¹H NMR Spectrum of Amide Compounds. University Chemistry, 2024, 39(3): 231-238. doi: 10.3866/PKU.DXHX202309020
10. [10]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
11. [11]
  Renxiao Liang , Zhe Zhong , Zhangling Jin , Lijuan Shi , Yixia Jia . A Palladium/Chiral Phosphoric Acid Relay Catalysis for the One-Pot Three-Step Synthesis of Chiral Tetrahydroquinoline. University Chemistry, 2024, 39(5): 209-217. doi: 10.3866/PKU.DXHX202311024
12. [12]
  Jiandong Liu , Zhijia Zhang , Mikhail Kamenskii , Filipp Volkov , Svetlana Eliseeva , Jianmin Ma . Research Progress on Cathode Electrolyte Interphase in High-Voltage Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(2): 100011-. doi: 10.3866/PKU.WHXB202308048
13. [13]
  Jingming Li , Bowen Ding , Nan Li , Nurgul . Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching. University Chemistry, 2024, 39(8): 263-269. doi: 10.3866/PKU.DXHX202312078
14. [14]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
15. [15]
  Zhiwen HU , Weixia DONG , Qifu BAO , Ping LI . Low-temperature synthesis of tetragonal BaTiO₃ for piezocatalysis. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 857-866. doi: 10.11862/CJIC.20230462
16. [16]
  Chunai Dai , Yongsheng Han , Luting Yan , Zhen Li , Yingze Cao . Ideological and Political Design of Solid-liquid Contact Angle Measurement Experiment. University Chemistry, 2024, 39(2): 28-33. doi: 10.3866/PKU.DXHX202306065
17. [17]
  Feiya Cao , Qixin Wang , Pu Li , Zhirong Xing , Ziyu Song , Heng Zhang , Zhibin Zhou , Wenfang Feng . Magnesium-Ion Conducting Electrolyte Based on Grignard Reaction: Synthesis and Properties. University Chemistry, 2024, 39(3): 359-368. doi: 10.3866/PKU.DXHX202308094
18. [18]
  Haiyang Zhang , Yanzhao Dong , Haojie Li , Ruili Guo , Zhicheng Zhang , Jiangjiexing Wu . Exploring the Integration of Chemical Engineering Principle Experiment with Cutting-Edge Research Achievements. University Chemistry, 2024, 39(10): 308-313. doi: 10.12461/PKU.DXHX202405035
19. [19]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
20. [20]
  Jiahe LIU , Gan TANG , Kai CHEN , Mingda ZHANG . Effect of low-temperature electrolyte additives on low-temperature performance of lithium cobaltate batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 719-728. doi: 10.11862/CJIC.20250023

Get Citation

PDF

XML

Metrics

PDF Downloads(2)
Abstract views(285)
HTML views(40)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142