Citation: JIANG Yan, FAN Guifang, DU Ran, LI Peipei, JIANG Li, ZHAO Gang, QI Panlun, LI Shizhong. Determination of sugars, organic acids and alcohols in microbial consortium fermentation broth from cellulose using high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2015, 33(8): 805-808. doi: 10.3724/SP.J.1123.2015.04038 shu

Determination of sugars, organic acids and alcohols in microbial consortium fermentation broth from cellulose using high performance liquid chromatography

JIANG Yan ^1,2 ,
FAN Guifang ^1,2 ,
DU Ran ^1,2 ,
LI Peipei ^1,2 ,
JIANG Li ^1,2 ,
ZHAO Gang ² ,
QI Panlun ³ ,
LI Shizhong ^1,2,,

1.
1. Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China;

Corresponding author: LI Shizhong,
Received Date: 22 April 2015

Fund Project: 国家高技术研究发展计划("863"计划)项目(2012AA101805) ("863"计划)项目(2012AA101805)国家国际科技合作专项项目(2012DFG61720,2012DFG61700). (2012DFG61720,2012DFG61700)

A high performance liquid chromatographic method was established for the determination of metabolites (sugars, organic acids and alcohols) in microbial consortium fermentation broth from cellulose. Sulfate was first added in the samples to precipitate calcium ions in microbial consortium culture medium and lower the pH of the solution to avoid the dissociation of organic acids, then the filtrates were effectively separated using high performance liquid chromatography. Cellobiose, glucose, ethanol, butanol, glycerol, acetic acid and butyric acid were quantitatively analyzed. The detection limits were in the range of 0.10-2.00 mg/L. The linear correlation coefficients were greater than 0.9996 in the range of 0.020 to 1.000 g/L. The recoveries were in the range of 85.41%-115.60% with the relative standard deviations of 0.22%-4.62% (n=6). This method is accurate for the quantitative analysis of the alcohols, organic acids and saccharides in microbial consortium fermentation broth from cellulose.
- high performance liquid chromatography (HPLC),
- sugars,
- organic acids,
- alcohols,
- cellulose degradation,
- microbial consortium,
- consolidated bioprocessing (CBP)
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