Citation: ZHU Wanyan, ZHANG Xin, YANG Juan, XU Wenyuan, XU Meiling. Simultaneous determination of multi-classes of veterinary drug residues in pork by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(9): 1002-1008. doi: 10.3724/SP.J.1123.2015.04033 shu

Simultaneous determination of multi-classes of veterinary drug residues in pork by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry

1.
Inspection and Quarantine Technology Center, Linyi Entry-Exit Inspection and Quarantine Bureau, Linyi 276034, China

Corresponding author: ZHU Wanyan,
Received Date: 22 April 2015

Fund Project: 山东出入境检验检疫局科研课题(SK201359). (SK201359)

An analytical method was established for the simultaneous determination of six classes of 33 veterinary drug residues in pork by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF MS). The QuEChERS method has been validated for the extraction. In this method, the sample was extracted by acetonitrile containing 5%(v/v) acetic acid and cleaned-up with C18 and NH₂ adsorbents. The extract was measured directly by UPLC-Q-TOF MS with electrospray ionization in positive mode. The compounds were separated on a ZORBAX SB-C18 column (100 mm×2.1 mm, 3.5 μm) with acetonitrile-0.1% formic acid containing 5 mmol/L ammonium acetate as mobile phases under gradient elution. The confirmatory analysis was carried out by determining the retention times and accurate masses of all the compounds and fragment ions upon Target MS/MS. The correlation coefficients of the 33 veterinary drugs were more than 0.99 within their linear ranges. The limits of quantification (LOQs) were 2.5-100 μg/kg. The recoveries ranged from 67.0% to 109.0% with the relative standard deviations (RSDs, n=6) not more than 15.1%. The method is rapid, sensitive, and suitable for the simultaneous determination of multi-classes of veterinary drugs in pork.
1. [1]
  [1] Xia M, Jia L, Ji Y P. Journal of Instrumental Analysis (夏敏, 贾丽, 季怡萍. 分析测试学报), 2004, 23(Z1): 217
2. [2]
  [2] Wang C Y, Wang Y P, Wang N, et al. Chinese Journal of Analytical Chemistry (王重洋, 王远鹏, 王宁, 等. 分析化学), 2013, 41(1): 83
3. [3]
  [3] Bu M N, Shi Z H, Kang J, et al. Journal of Instrumental Analysis (卜明楠, 石志红, 康健, 等. 分析测试学报), 2012, 31(5): 552
4. [4]
  [4] Wang L, Li Y Q, Wang H B, et al. Chinese Journal of Analytical Chemistry (王炼, 黎源倩, 王海波, 等. 分析化学), 2011, 39(2): 203
5. [5]
  [5] Yan L Z, Zhao Y B, Fu Y, et al. Chinese Journal of Analysis Laboratory (晏利芝, 赵永彪, 富玉, 等. 分析试验室), 2011, 30(1): 2011
6. [6]
  [6] Li N, Zhang Y T, Liu L, et al. Chinese Journal of Chromatography (李娜, 张玉婷, 刘磊, 等. 色谱), 2014, 32(12): 1313
7. [7]
  [7] Cao Y F, Kang J, Chang Q Y, et al. Chinese Journal of Chromatography (曹亚飞, 康健, 常巧英, 等. 色谱), 2015, 33(2): 132
8. [8]
  [8] Li X J, Peng T, Li C J. Journal of Instrumental Analysis (李晓娟, 彭涛, 李重九. 分析测试学报), 2012, 31(5): 628
9. [9]
  [9] Liu C, Chen Y, Li X W, et al. Chinese Journal of Food Hygiene (刘畅, 陈燕, 李晓雯, 等. 中国食品卫生杂志), 2014, 26(5): 464
10. [10]
  [10] Peng L, Wu N P, Zhang C W, et al. Chinese Journal of Veterinary Medicine (彭丽, 吴宁鹏, 张崇威, 等. 中国兽药杂志), 2014, 48(9): 45
11. [11]
  [11] Gao F D, Zhao Y, Shao B, et al. Chinese Journal of Chromatography (高馥碟, 赵妍, 邵兵, 等. 色谱), 2012, 30(6): 560
12. [12]
  [12] Anastassiades M, Lehotay S J. J AOAC Int, 2003, 86(2): 412
13. [13]
  [13] Cao H, Chen X Z, Zhu Y, et al. Food Science and Technology (曹慧, 陈小珍, 朱岩, 等. 食品科技), 2013, 38(6): 323
14. [14]
  [14] Wang W, Shi Z H, Kang J, et al. Chinese Journal of Analysis Laboratory (王伟, 石志红, 康健, 等. 分析试验室), 2013, 32(4): 82
15. [15]
  [15] Wang L Q, He L M, Zeng Z L, et al. Journal of Chinese Mass Spectrometry Society (王立琦, 贺利民, 曾振灵, 等. 质谱学报), 2011, 32(6): 321
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沈阳化工大学材料科学与工程学院沈阳 110142