Citation: ZHU Jingbo, LIU Baoyue, SHAN Shibo, DING Yan, KOU Zinong, XIAO Wei. Development of an automatic vacuum liquid chromatographic device and its application in the separation of the components from Schisandra chinensis (Turcz) Baill.[J]. Chinese Journal of Chromatography, ;2015, 33(8): 864-868. doi: 10.3724/SP.J.1123.2015.04026 shu

Development of an automatic vacuum liquid chromatographic device and its application in the separation of the components from Schisandra chinensis (Turcz) Baill.

ZHU Jingbo ^1,2,, ,
LIU Baoyue ¹ ,
SHAN Shibo ² ,
DING Yan ^1,2 ,
KOU Zinong ^2,3 ,
XIAO Wei ⁴

1.
1. School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China;

Corresponding author: ZHU Jingbo,
Received Date: 16 April 2015

Fund Project: 大连市科技计划项目(2014E12SF065) (2014E12SF065)辽宁省高等学校重大科技平台项目(辽教发[2011]191号). (辽教发[2011]191号)

In order to meet the needs of efficient purification of products from natural resources, this paper developed an automatic vacuum liquid chromatographic device (AUTO-VLC) and applied it to the component separation of petroleum ether extracts of Schisandra chinensis (Turcz) Baill. The device was comprised of a solvent system, a 10-position distribution valve, a 3-position changes valve, dynamic axis compress chromatographic columns with three diameters, and a 10-position fraction valve. The programmable logic controller (PLC) S7-200 was adopted to realize the automatic control and monitoring of the mobile phase changing, column selection, separation time setting and fraction collection. The separation results showed that six fractions (S1-S6) of different chemical components from 100 g Schisandra chinensis (Turcz) Baill. petroleum ether phase were obtained by the AUTO-VLC with 150 mm diameter dynamic axis compress chromatographic column. A new method used for the VLC separation parameters screened by using multiple development TLC was developed and confirmed. The initial mobile phase of AUTO-VLC was selected by taking R_f of all the target compounds ranging from 0 to 0.45 for fist development on the TLC; gradient elution ratio was selected according to k value (the slope of the linear function of R_f value and development times on the TLC) and the resolution of target compounds; elution times (n) were calculated by the formula n≈ Δ R_f/k. A total of four compounds with the purity more than 85% and 13 other components were separated from S5 under the selected conditions for only 17 h. Therefore, the development of the automatic VLC and its method are significant to the automatic and systematic separation of traditional Chinese medicines.
- vacuum liquid chromatography (VLC),
- automatic device,
- application,
- Schisandra chinensis (Turcz) Baill.,
- separation
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