Citation: ZHANG Huanhuan, LI Jiang, ZHAO Shan, DING Xiaojing, WANG Zhi. Rapid determination of lactose, sucrose, glucose and fructose in foods by capillary zone electrophoresis with indirect ultraviolet detection[J]. Chinese Journal of Chromatography, ;2015, 33(8): 816-821. doi: 10.3724/SP.J.1123.2015.03042 shu

Rapid determination of lactose, sucrose, glucose and fructose in foods by capillary zone electrophoresis with indirect ultraviolet detection

ZHANG Huanhuan ^1,2 ,
LI Jiang ² ,
ZHAO Shan ² ,
DING Xiaojing ^2,, ,
WANG Zhi ^1,,

1.
1. College of Science, Agricultural University of Hebei, Baoding 071001, China;

Corresponding author: DING Xiaojing, WANG Zhi,
Received Date: 31 March 2015

Fund Project: 北京市卫生系统高层次卫生技术人才培养项目(2013-2-036). (2013-2-036)

A new and rapid method for the simultaneous determination of lactose, sucrose, glucose and fructose by capillary zone electrophoresis (CZE) with indirect ultraviolet detection was developed. The separation was completed with an uncoated fused-silica capillary with 30.2 cm of total length (effective length of 20 cm)×50 μm. The separation buffer consisted of 4 mmol/L potassium sorbate, 10 mmol/L sodium phosphate, 30 mmol/L NaOH (pH 12.56) and 0.5 mmol/L hexadecytrimethylammonium bromide (CTAB). The separation was performed at a voltage of -8 kV with the ultraviolet detection at 254 nm. The analysis of the four carbohydrates was completed within 10 min. The limits of detection (S/N=3) for lactose, sucrose, glucose and fructose were 50, 75, 25 and 25 mg/L, and the limits of quantification (S/N=10) were 150, 225, 75 and 75 mg/L, respectively. The average recoveries for the four carbohydrates were in the range of 87.0%-107.0% with the relative standard deviations of 1.2%-4.7%. No organic solvent was consumed throughout the whole process of the analysis. The method was used for the analysis of nine food samples and a quality control sample. The results demonstrated that the method is simple, rapid, accurate, and suitable for the routine analysis of the four carbohydrates in food samples.
- capillary zone electrophoresis (CZE),
- indirect ultraviolet detection,
- lactose,
- sucrose,
- glucose,
- fructose,
- foods
1. [1]
  [1] Ren L P, Song G Y. Chinese General Practice (任路平, 宋光耀. 中国全科医学), 2011, 14(4B): 1278
2. [2]
  [2] Zuo T M. China Inspection and Quarantine (左天明. 中国检验检疫), 2012(9): 19
3. [3]
  [3] GB/T 5009.7-2008
4. [4]
  [4] GB/T 5009.8-2008
5. [5]
  [5] Lin H, Yan C R, Xu C X, et al. Food Science (林慧, 颜春荣, 徐春祥, 等. 食品科学), 2013, 34(12): 286
6. [6]
  [6] Wang F Q, Yang H X, Wang Y Z. Chinese Journal of Chromatography (王凤芹, 杨航仙, 汪以真. 色谱), 2013, 31(1): 53
7. [7]
  [7] Shanmugavelan P, Kim S Y, Kim J B, et al. Carbohyd Res, 2013, 380: 112
8. [8]
  [8] Zakharova A M, Grinshtein I L, Kartsova L A. J Anal Chem, 2013, 68(12): 1081
9. [9]
  [9] Oliver J D, Gaborieau M, Hilder E F, et al. J Chromatogr A, 2013, 1291: 179
10. [10]
  [10] Yilmaz M T, Tatlisu N B, Toker O S, et al. Food Res Int, 2014, 64: 634
11. [11]
  [11] Ma C M, Sun Z, Chen C B, et al. Food Chem, 2014, 145: 784
12. [12]
  [12] Ghfar A A, Wabaidur S M, Ahmed A Y B H, et al. Food Chem, 2015, 176: 487
13. [13]
  [13] Ding H L, Li C, Jin P, et al. Chinese Journal of Chromatography (丁洪流, 李灿, 金萍, 等. 色谱), 2013, 31(8): 804
14. [14]
  [14] Eggleston G. Food Chem, 1999, 65: 483
15. [15]
  [15] Zheng H L, Deng B H, Xiao G Q, et al. Food Science (郑惠玲, 邓宝浣, 肖桂秋, 等. 食品科学), 2014, 35(6): 180
16. [16]
  [16] Xi L L, Wang F L, Zhu Z Y, et al. Talanta, 2014, 119: 440
17. [17]
  [17] Guignard C, Jouve L, Bogéat-Triboulot M B, et al. J Chromatogr A, 2005, 1085: 137
18. [18]
  [18] Fa Y, Yang H Y, Ji C S, et al. Anal Chim Acta, 2013, 798: 97
19. [19]
  [19] Li J, Chen M L, Zhu Y. J Chromatogr A, 2007, 1155: 50
20. [20]
  [20] Xu Y, Zang Y, Jiang T, et al. Chinese Journal of Chromatography (徐颖, 臧颖, 姜婷, 等. 色谱), 2014, 32(12): 1400
21. [21]
  [21] Chen T, Ding X J, Li Y Z, et al. Chinese Journal of Chromatography (陈桐, 丁晓静, 李一正, 等. 色谱), 2014, 32(6): 666
22. [22]
  [22] Chen Y. Capillary Electrophoresis Technology and Application. 2nd ed: Beijing: Chemical Industry Press (陈义. 毛细管电泳技术及应用. 2版. 北京: 化学工业出版社), 2006
23. [23]
  [23] Rizelio V M, Tenfen L, Silveira R D, et al. Talanta, 2012, 93: 62
24. [24]
  [24] Boyce M C, Breadmore M, Macka M, et al. Electrophoresis, 2000, 21: 3073
25. [25]
  [25] Macka M, Johns C, Doble P, et al. LC GC CE Currents, 2001, 19(1): 38
26. [26]
  [26] Grill E, Huber C, Oefner P, et al. Electrophoresis, 1994, 14: 1004
27. [27]
  [27] Sheng L, Su B Q. Chinese Journal of Chemical Education (盛丽, 苏碧泉. 化学教育), 2004, 25(7): 8
28. [28]
  [28] Xu X, Kok W T, Poppe H. J Chromatogr A, 1997, 786: 333
29. [29]
  [29] Colón L A, Dadoo R, Zare R N. Anal Chem, 1993, 65: 478
30. [30]
  [30] Schellinger A P, Carr P W. LC GC North America, 2004, 22(6): 544
31. [31]
  [31] Weston A, Brown P R. HPLC and CE Principles and Practice. San Diego: Academic Press, 1997: 145
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