Citation: WANG Jiabin, WU Fangling, ZHAO Qi. Synchronous extraction and determination of phenoxy acid herbicides in water by on-line monolithic solid phase microextraction-high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2015, 33(8): 849-855. doi: 10.3724/SP.J.1123.2015.03030 shu

Synchronous extraction and determination of phenoxy acid herbicides in water by on-line monolithic solid phase microextraction-high performance liquid chromatography

1.
Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China

Corresponding author: WANG Jiabin,
Received Date: 23 March 2015

Fund Project: 国家自然科学基金青年基金项目(31401609). (31401609)

A C18 monolithic capillary column was utilized as the solid phase microextraction column to construct an in-tube SPME-HPLC system which was used to simultaneously extract and detect five phenoxy acid herbicides, including 2,4-dichlorophenoxyacetic acid (2,4-D), 2-(2-chloro)-phenoxy propionic acid (2,2-CPPA), 2-(3-chloro)-phenoxy propionic acid (2,3-CPPA), phenoxy propionic acid (PPA) and 2-(2,4-dichlorophenoxy)propionic acid (2,4-DP). The operating parameters of the in-tube SPME-HPLC system, including the length of the monolithic column, the sampling flow rate, the sampling time, the elution flow rate and the elution time, had been investigated in detail. The optimized operating parameters of the in-tube SPME-HPLC system were as follow: the length of the monolithic column was 20 cm, the sampling flow rate was 0.04 mL/min, sampling time was 13 min; the elution flow rate was 0.02 mL/min, elution time was 5 min. Under the optimized conditions, the detection limits of the five phenoxy acid herbicides were as follows: 9 μg/L for PPA, 4 μg/L for 2,2-CPPA, 4 μg/L for 2,3-CPPA, 5 μg/L for 2,4-D, 5 μg/L for 2,4-DP. Compared with the HPLC method with direct injection, the combined system showed a good enrichment factors to the analytes. The recoveries of the five phenoxy acid herbicides were between 79.0% and 98.0% (RSD≤3.9%). This method was successfully used to detect the five phenoxy acid herbicides in water samples with satisfactory results.
1. [1]
  [1] Kuang H. [MS Dissertation]. Beijing: China Agricultural University (匡华. [硕士学位论文]. 北京: 中国农业大学), 2006
2. [2]
  [2] Miller W R, Sharpe R M. Endocrine-Related Cancer, 1998, 5(2): 69
3. [3]
  [3] GB 5749-2006
4. [4]
  [4] WHO. Guidelines for Drinking-Water Quality. 4th ed. 2011: 347. [2015-03-23]. http://www.who.int/water_sanitation_health/publications/2011/dwq_guidelines/en/
5. [5]
  [5] Li R J, Zhang X M, Yu J L, et al. Modern Agrochemicals (李瑞娟, 张晓梅, 于建垒, 等. 现代农药), 2011, 10(2): 35
6. [6]
  [6] Hou X, Han M, Dai X H, et al. Food Chem, 2013, 138: 1198
7. [7]
  [7] Shamsipur M, Fattahi N, Pirsaheb M, et al. J Sep Sci, 2012, 35: 2718
8. [8]
  [8] Yeh M K, Lin S L, Leong M I, et al. Anal Sci, 2011, 27: 49
9. [9]
  [9] Anagnostopoulos C J, Liapis K, Haroutounian S, et al. J Liq Chromatogr Related Technol, 2013, 36: 315
10. [10]
  [10] McManus S L, Moloney M, Richards K G, et al. Molecules, 2014, 19: 20627
11. [11]
  [11] Kittlaus S, Schimanke J, Kempe G, et al. J Chromatogr A, 2013, 1283: 98
12. [12]
  [12] Tabani H, Fakhari A R, Zand E. Anal Methods, 2013, 5: 1548
13. [13]
  [13] Zhang Y, Jiao B. J Sep Sci, 2013, 36: 3067
14. [14]
  [14] Deng A P, Yang H. Sens Actuators B, 2007, 124: 202
15. [15]
  [15] Zhang Z, Yang M J, Pawliszyn J. Anal Chem, 1994, 66(17): 844A
16. [16]
  [16] Vuckovic D, Cudjoe E, Hein D, et al. Anal Chem, 2008, 80(18): 6870
17. [17]
  [17] Li X, Zhang Y Q, Ai L F. Chinese Journal of Chromatography (李欣, 张瑶琴, 艾连峰, 等. 色谱), 2015, 33(6): 590
18. [18]
  [18] Svec F. J Chromatogr A, 2010, 1217: 902
19. [19]
  [19] Jiang Z, Smith N W, Liu Z. J Chromatogr A, 2011, 1218: 2350
20. [20]
  [20] Luo W Q, Xiao X H, Du Z, et al. Chinese Journal of Chromatography (罗伟强, 肖小华, 杜卓, 等. 色谱), 2014, 32(4): 407
21. [21]
  [21] Wang X, Li X, Li Z, et al. Anal Chem, 2014, 86: 4739
22. [22]
  [22] Peng Y, He H, Sun C, et al. Chinese Journal of Analytical Chemistry (彭英, 何欢, 孙成, 等. 分析化学), 2013, 41(4): 529
23. [23]
  [23] Ou J, Liu Z, Wang H, et al. Electrophoresis, 2015, 36: 62
24. [24]
  [24] Wang M M, Yan X P. Anal Chem, 2012, 84: 39
25. [25]
  [25] Wang J, Jia W, Lin X, et al. Electrophoresis, 2013, 34: 3293
26. [26]
  [26] Wang J, Lü H, Lin X, et al. Electrophoresis, 2008, 29(4): 928
27. [27]
  [27] Lin J, Wu X, Lin X, et al. J Chromatogr A, 2007, 1169: 220
28. [28]
  [28] Fan Y, Feng Y Q, Da S L, et al. Anal Chim Act, 2004, 523: 251
29. [29]
  [29] Peng X J, Pang J S, Deng A H. Chinese Journal of Chromatography (彭晓俊, 庞晋山, 邓爱华. 色谱), 2011, 29(12): 1199
1. [1]
  Gengjia Chen , Junjie Ou . Application of the van Deemter Equation in Instrumental Analysis Teaching: A Case of Organic Polymer Monolithic Columns. University Chemistry, 2025, 40(11): 362-368. doi: 10.12461/PKU.DXHX202502003
2. [2]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
3. [3]
  Tong Wang , Liangyu Hu , Shiqi Chen , Xinqiang Fu , Rui Wang , Kun Li , Shuangyan Huan . Determination of Benzenediol Isomers in Cosmetics Using High-Performance Liquid Chromatography Empowered by “Mathematical Separation”. University Chemistry, 2026, 41(1): 9-19. doi: 10.12461/PKU.DXHX202503128
4. [4]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
5. [5]
  Siming Bian , Sijie Luo , Junjie Ou . Application of van Deemter Equation in Instrumental Analysis Teaching: A New Type of Core-Shell Stationary Phase. University Chemistry, 2025, 40(3): 381-386. doi: 10.12461/PKU.DXHX202406087
6. [6]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
7. [7]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
8. [8]
  Lingyu Chang , Yanfang Lang , Yuyan Zhu , Jie Wang , Ying Guo , Die Wang , Peng Ding , Yueming Zhou , Zhixiang Gong , Shujuan Liu . Machine Learning-Optimized Microcolumn Ion Exchange Chromatography for Trace Arsenic Determination. University Chemistry, 2026, 41(1): 76-84. doi: 10.12461/PKU.DXHX202506023
9. [9]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
10. [10]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
11. [11]
  Xiaojun Liu , Lang Qin , Yanlei Yu . Dynamic Manipulation of Photonic Bandgaps in Cholesteric Liquid Crystal Microdroplets for Applications. Acta Physico-Chimica Sinica, 2024, 40(5): 2305018-0. doi: 10.3866/PKU.WHXB202305018
12. [12]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019
13. [13]
  Yajie Li , Bin Chen , Yiping Wang , Hui Xing , Wei Zhao , Geng Zhang , Siqi Shi . Inhibiting Dendrite Growth by Customizing Electrolyte or Separator to Achieve Anisotropic Lithium-Ion Transport: A Phase-Field Study. Acta Physico-Chimica Sinica, 2024, 40(3): 2305053-0. doi: 10.3866/PKU.WHXB202305053
14. [14]
  Binbin Liu , Yang Chen , Tianci Jia , Chen Chen , Zhanghao Wu , Yuhui Liu , Yuhang Zhai , Tianshu Ma , Changlei Wang . Hydroxyl-functionalized molecular engineering mitigates 2D phase barriers for efficient wide-bandgap and all-perovskite tandem solar cells. Acta Physico-Chimica Sinica, 2026, 42(1): 100128-0. doi: 10.1016/j.actphy.2025.100128
15. [15]
  Jiayao Wang , Guixu Pan , Ning Wang , Shihan Wang , Yaolin Zhu , Yunfeng Li . Preparation of donor-π-acceptor type graphitic carbon nitride photocatalytic systems via molecular level regulation for high-efficient H₂O₂ production. Acta Physico-Chimica Sinica, 2025, 41(12): 100168-0. doi: 10.1016/j.actphy.2025.100168
16. [16]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
17. [17]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
18. [18]
  Sumiya Akter Dristy , Md Ahasan Habib , Shusen Lin , Mehedi Hasan Joni , Rutuja Mandavkar , Young-Uk Chung , Md Najibullah , Jihoon Lee . Exploring Zn doped NiBP microspheres as efficient and stable electrocatalyst for industrial-scale water splitting. Acta Physico-Chimica Sinica, 2025, 41(7): 100079-0. doi: 10.1016/j.actphy.2025.100079
19. [19]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-0. doi: 10.3866/PKU.WHXB202309036
20. [20]
  Chunai Dai , Yongsheng Han , Luting Yan , Zhen Li , Yingze Cao . Ideological and Political Design of Solid-liquid Contact Angle Measurement Experiment. University Chemistry, 2024, 39(2): 28-33. doi: 10.3866/PKU.DXHX202306065

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(1467)
HTML views(61)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142