Citation: WANG Huanhuan, LÜ Yayao, PENG Bo, QIAN Xiaohong, ZHANG Yangjun. Progress in quantitative methods based on liquid chromatography-mass spectrometry for drug metabolizing enzymes in human liver microsomes[J]. Chinese Journal of Chromatography, ;2015, 33(6): 553-557. doi: 10.3724/SP.J.1123.2015.03023 shu

Progress in quantitative methods based on liquid chromatography-mass spectrometry for drug metabolizing enzymes in human liver microsomes

WANG Huanhuan ^1,2 ,
LÜ Yayao ² ,
PENG Bo ² ,
QIAN Xiaohong ² ,
ZHANG Yangjun ^1,2,,

1.
1. Guangxi Medical University, Nanning 530021, China;

Corresponding author: ZHANG Yangjun,
Received Date: 17 March 2015

Fund Project: 国家重大科学计划项目(2012CB910603,2013CB911204) (2012CB910603,2013CB911204)国家重大科学仪器设备开发专项项目(2011YQ030139,2012YQ-12004407,2011YQ06008408,2013YQ14040506). (2011YQ030139,2012YQ-12004407,2011YQ06008408,2013YQ14040506)

Cytochrome P450 (CYP) enzymes and uridine 5-diphospho-glucuronosyltransferase (UGT) enzymes are critical enzymes for drug metabolism. Both chemical drugs and traditional Chinese medicines are converted to more readily excreted compounds by drug metabolizing enzymes in human livers. Because of the disparate expression of CYP and UGT enzymes among different individuals, accurate quantification of these enzymes is essential for drug pharmacology, drug-drug interactions and drug clinical applications. The research progress in quantitative methods based on liquid chromatography-mass spectrometry for drug metabolizing enzymes in human liver microsomes in the recent decade is reviewed.
- drug metabolizing enzymes,
- quantitative methods,
- liquid chromatography-mass spectrometry (LC-MS),
- review
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