Citation: WANG Pingya, ZHOU Yong, DAI Yifei, HUANG Li, ZHAO Qiaoling. Rapid screening of 28 acidic artificial dyes in fishery products by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(8): 822-829. doi: 10.3724/SP.J.1123.2015.03022 shu

Rapid screening of 28 acidic artificial dyes in fishery products by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry

1.
Zhoushan Institute for Food and Drug Control, Zhoushan Institute of Calibration and Testing for Quality and Technology Supervision, Zhoushan 316021, China

Corresponding author: DAI Yifei,
Received Date: 17 March 2015

Fund Project: 国家科技部公益专项(201210060) (201210060)舟山市公益类科技项目(2013C31050). (2013C31050)

An analytical method was established for the simultaneous determination of 28 acidic artificial dyes in fishery products by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF MS). The analytes in fishery products were extracted with ethyl alcohol containing 20% (v/v) ammonia water and 10% (v/v) water. Then the extract solution was purified according to amination adsorbent matrix dispersion method. The compounds were separated by an Eclipse Plus-C₁₈ column with acetonitrile and 0.1% (v/v) formic acid-5 mmol/L ammonium acetate solution as the mobile phases, and measured by UPLC-Q-TOF MS with electrospray ionization in negative mode. As a result, the limits of quantification (LOQs, S/N=10) of the target compounds were 20-100 μg/kg in fishery products. The 28 analytes behaved linearly in wide-range with the correlation coefficients more than 0.991 as well as the relative standard deviations (RSDs) of the peak areas less than 5.61% (n=6). The average recoveries of the 28 analytes spiked at three levels were ranged from 70.24% to 106.47% with RSDs of 5.62%-9.65% (n=6). This method can be applied to the analysis of the 28 acidic artificial dyes in fishery products due to its fastness, simplicity and relatively high sensitivity.
1. [1]
  [1] Ma M, Luo X B, Chen B, et al. J Chromatogr A, 2006, 1103: 170
2. [2]
  [2] Feng F, Zhao Y S, Yong W, et al. J Chromatogr B, 2011, 879: 1813
3. [3]
  [3] Lü D M, Ding Y L, Zhan S, et al. Modern Instruments & Medical Treatment (吕东明, 丁云连, 詹晟, 等. 现代仪器与医疗), 2013, 19(2): 52
4. [4]
  [4] Yang L, Chen Q J, Ding X R, et al. Science and Technology of Food Industry (杨琳, 陈青俊, 丁献荣, 等. 食品工业科技), 2011, 32(2): 350
5. [5]
  [5] Zhang Y D, Chang C L, Guo Q L, et al. Chinese Journal of Chromatography (张一丁, 常翠兰, 郭启雷, 等. 色谱), 2014, 32(4): 438
6. [6]
  [6] Huang L Y, Zhang X B, Chen X Z, et al. Journal of Instrumental Analysis (黄丽英, 张晓波, 陈小珍, 等. 分析测试学报), 2013, 32(6): 687
7. [7]
  [7] Tu H Y, Xiao H L, Zhu S D, et al. China Food (屠海云, 肖海龙, 朱顺达, 等. 中国食品), 2011(10): 82
8. [8]
  [8] Johns C, Macka M, Haddad P R. J Chromatogr A, 2003, 997: 87
9. [9]
  [9] Ni R, Yang L B, Liu L X. Chinese Journal of Health Laboratory Technology (倪蓉, 杨龙彪, 刘兰侠. 中国卫生检验杂志), 2010, 20(5): 991
10. [10]
  [10] Zhao F, Gao G H, Na H Q, et al. Chinese Journal of Chromatography (赵飞, 高广慧, 那海秋, 等. 色谱), 2013, 31(5): 490
11. [11]
  [11] Wang D Y, Yang X L, Li X Q, et al. Physical Testing and Chemical Analysis Part B: Chemical Analysis (王冬妍, 杨晓丽, 李兴权, 等. 理化检验-化学分册), 2010, 46(9): 1034
12. [12]
  [12] Zhao R, Li B, Zhao H Y, et al. Chinese Journal of Food Hygiene (赵榕, 李兵, 赵海燕, 等. 中国食品卫生杂志), 2010, 22(4): 305
13. [13]
  [13] Li B R, Feng J L, Pan Z Q, et al. Chinese Journal of Health Laboratory Technology (李帮锐, 冯家力, 潘振球, 等. 中国卫生检验杂志), 2007, 17(4): 579
14. [14]
  [14] Hu L, Lei S R, Guo L A, et al. Chinese Journal of Analytical Chemistry (胡莉, 雷绍荣, 郭灵安, 等. 分析化学), 2013, 41(1): 110
15. [15]
  [15] Zheng L Y, Chen J J, Xu J L, et al. Chinese Journal of Chromatography (郑立洋, 陈娟娟, 徐继林, 等. 色谱), 2014, 32(9): 992
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沈阳化工大学材料科学与工程学院沈阳 110142