Citation: TANG Chunlan, WANG Li, CHENG Mengchun, LIU Xinxin, XIAO Hongbin. Determination of N⁶-(4-hydroxybenzyl) adenine riboside in rat plasma by ultra performance liquid chromatography- quadrupole time of flight mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(7): 699-703. doi: 10.3724/SP.J.1123.2015.03017 shu

Determination of N⁶-(4-hydroxybenzyl) adenine riboside in rat plasma by ultra performance liquid chromatography- quadrupole time of flight mass spectrometry

1.
1. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;

Corresponding author: XIAO Hongbin,
Received Date: 12 March 2015

Fund Project: 国家自然科学基金项目(81001629) (81001629)重大新药创制专项课题(2014XZ09304-307). (2014XZ09304-307)

An ultra performance liquid chromatography-quadrupole time of flight mass spectrometry (UPLC-QTOF-MS) method was developed and validated for the determination of N⁶-(4-hydroxybenzyl) adenine riboside and its pharmacokinetics in rat plasma. The chromatographic conditions were optimized. The separation was performed on an Agilent ZORBAX SB-C18 column(150 mm×3 mm, 1.8 μm) with a gradient elution of 0.2%(v/v) formic acid aqueous solution and acetonitrile as the mobile phases at a flow rate of 0.35 mL/min. The detection was accomplished in positive mode with electrospray ionization (ESI) by UPLC-QTOF-MS, and 6-benzylamino purine was used as the internal standard (IS). The results showed that the linear range of calibration curve was 0.625-160 ng/mL for N⁶-(4-hydroxybenzyl) adenine riboside in rat plasma with the correlation coefficient more than 0.99. The recoveries were 88.41%-108.26%. The limit of detection was 0.1 ng/mL. The intra-day and inter-day precisions (RSDs) were less than 6%, and intra-day and inter-day accuracies (REs, RE=(measured concentration-spiked concentration)/spiked concentration×100%) were less than ±15%. The method is rapid, sensitive and accurate for the quantitation of N⁶-(4-hydroxybenzyl) adenine riboside, which can be used for the study of pharmacokinetics of N⁶-(4-hydroxybenzyl) adenine riboside.
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Determination of N6-(4-hydroxybenzyl) adenine riboside in rat plasma by ultra performance liquid chromatography- quadrupole time of flight mass spectrometry

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通讯作者: 陈斌, bchen63@163.com

Determination of N⁶-(4-hydroxybenzyl) adenine riboside in rat plasma by ultra performance liquid chromatography- quadrupole time of flight mass spectrometry