Citation: XU Lili, ZHONG Minghua, CHEN Xiaojing. Preparation and chromatographic performance of a eugenol-bonded silica gel stationary phase for high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2015, 33(5): 461-467. doi: 10.3724/SP.J.1123.2015.01039 shu

Preparation and chromatographic performance of a eugenol-bonded silica gel stationary phase for high performance liquid chromatography

1.
Department of Chemistry, Hanshan Normal University, Chaozhou 521041, China

Corresponding author: XU Lili,
Received Date: 28 January 2015

Fund Project: 潮州市科技局引导计划项目(2013H04). (2013H04)

A eugenol-bonded silica gel stationary phase (EGSP) for high performance liquid chromatography (HPLC) has been synthesized by the solid-liquid successive reaction method. The preparation process included two steps: firstly, γ-glycidoxypropyltrimethoxy-silane (KH-560) was covalently attached to the surface of spherical silica gel. Then the bonded silica gel continued to react with eugenol ligand, which was a plant active component, and obtained EGSP. The structure of EGSP was characterized by elemental analysis, thermogravimetric analysis and Fourier transform infrared spectroscopy. Using naphthalene as a probe, the column efficiency was tested under the mobile phase of acetonitrile-water (35:65, v/v) at a flow rate of 0.8 mL/min. The chromatographic properties and the retention mechanism of EGSP were evaluated by using neutral, basic and acidic analytes as solute probes. Meanwhile, the comparative study with C₁₈ column and phenyl column was also carried out under the same chromatographic conditions. The result showed that the eugenol ligand was successfully bonded to the surface of silica gel with a 0.28 mmol/g of bonded amount, and the theoretical plate number of EGSP column was about 24707 N/m. The EGSP appeared to be a kind of excellent reversed-phase stationary phase with suitable hydrophobicity and various synergistic sites. The eugenol ligand bonded on silica gel could first provideπ-πinteraction sites for different analytes because of its benzene ring and alkenyl. In addition, the methoxy groups of eugenol were responsible for dipole-dipole and hydrogen-bonding interactions between the ligand and solutes in the effective separation process. Comparing with traditional C₁₈ column and phenyl column, EGSP has an advantage in the fast separation of polar compounds under simple experimental conditions.
- high performance liquid chromatography (HPLC),
- eugenol,
- bonded silica gel stationary phase,
- chromatographic property,
- retention mechanism
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沈阳化工大学材料科学与工程学院沈阳 110142