Citation: JIN Zuyao, LÜ Yayao, ZHOU Shanshan, HAO Feiran, FU Bin, YING Wantao, QIAN Xiaohong, ZHANG Yangjun. A simple preparation method of an electric heating apparatus for heating capillary chromatographic columns and its application in liquid chromatography-mass spectrometry system[J]. Chinese Journal of Chromatography, ;2015, 33(6): 563-570. doi: 10.3724/SP.J.1123.2015.01036 shu

A simple preparation method of an electric heating apparatus for heating capillary chromatographic columns and its application in liquid chromatography-mass spectrometry system

JIN Zuyao ^1,2 ,
LÜ Yayao ² ,
ZHOU Shanshan ² ,
HAO Feiran ² ,
FU Bin ² ,
YING Wantao ² ,
QIAN Xiaohong ² ,
ZHANG Yangjun ^1,2,,

1.
1. Anhui Medical University, Hefei 230032, China;

Corresponding author: ZHANG Yangjun,
Received Date: 27 January 2015

Fund Project: 国家重大科学计划项目(2012CB910603) (2012CB910603)国家高技术研究发展规划项目(2012AA020202) (2012AA020202)国家重大科学仪器设备开发专项项目(2011YQ09000504,2012YQ12004407,2011YQ030139,2011YQ06008408) (2011YQ09000504,2012YQ12004407,2011YQ030139,2011YQ06008408)国家自然科学基金项目(21275519,20735005,31100591). (21275519,20735005,31100591)

For deep coverage of proteome, especially in performing qualitative identification and quantitative analysis of low-abundance proteins, the most commonly used method is the application of a longer capillary chromatographic column or a capillary column packed with smaller particle sizes. However, this causes another problem, the very high back pressure which results in liquid leaks in some connection parts in a liquid chromatograph. To solve this problem, an electric heating apparatus was developed to raise the temperature of a capillary column for reducing its back pressure, which was further applied in a capillary high performance liquid chromatography-tandem mass spectrometry system (cHPLC-MS/MS), and evaluated in the terms of chromatographic column back pressure and chromatographic column efficiency using bovine serum albumin (BSA) tryptic digests and yeast tryptic digests, separately. The results showed that at the optimum current, our electric heating apparatus could reduce the column pressure of a capillary column packed with 3 μm packing materials by at least 50% during the separation of BSA tryptic digestion and yeast tryptic digestion, compared with that without electric heating. The column efficiency was also increased slightly. This suggested that the electric heating apparatus can significantly reduce the column pressure, which provides an efficient way to use capillary chromatographic columns packed with smaller sizes of particles at a lower pressure.
1. [1]
  [1] Aebersold R, Mann M. Nature, 2003, 422(6928): 198
2. [2]
  [2] Lenz C, Urlaub H. Expert Rev Proteomics, 2014, 11(4): 409
3. [3]
  [3] Huuang J F, Wang F J, Ye M L, et al. J Chromatogr A, 2014, 1372C: 1
4. [4]
  [4] Zhou F, Lu Y, Ficarro S B, et al. Nat Commun, 2013, 4: 2171
5. [5]
  [5] Wang H, Yang Y, Li Y, et al. J Proteome Res, 2015, 14(2): 829
6. [6]
  [6] Bruns S, Frabklin E G, Grinias J P, et al. J Chromatogr A, 2013, 1318: 189
7. [7]
  [7] DOrazio G, Rocco A, Fanali S. J Chromatogr A, 2012, 1228: 213
8. [8]
  [8] Burgess M W, Keshishian H, Mani D R, et al. Mol Cell Proteomics, 2014, 13(4): 1137
9. [9]
  [9] Scott Kephart T, Dasgupta P K. Talanta, 2002, 56(6): 977
10. [10]
  [10] Fu Y, Chen N W. China Measurement Technology (富玉, 陈能武. 中国测试技术), 2007, 33(3): 36
11. [11]
  [11] Liu G H. Natural Gas Chemical Industry (刘光会. 天然气化工), 2000, 25(6): 46
12. [12]
  [12] Warren F V. Anal Chem, 1988, 60(24): 2821
13. [13]
  [13] Anita F D, Horvath C. J Chromatogr, 1988, 435(1): 1
14. [14]
  [14] Yan B, Zhao J. Anal Chem, 2000, 72(6): 1253
15. [15]
  [15] Yang Y. Anal Chim Acta, 2006, 558(1/2): 7
16. [16]
  [16] Cheng H D, Xing Z F, Li T, et al. Fujian Analysis & Testing (成洪达, 邢占芬, 李彤, 等. 福建分析测试), 2009, 18(1): 16
17. [17]
  [17] Rogeterg M, Wilson S R, Malerod H, et al. J Chromatogr A, 2011, 1218(41): 7281
1. [1]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
2. [2]
  Yaping ZHANG , Tongchen WU , Yun ZHENG , Bizhou LIN . Z-scheme heterojunction β-Bi₂O₃ pillared CoAl layered double hydroxide nanohybrid: Fabrication and photocatalytic degradation property. Chinese Journal of Inorganic Chemistry, 2025, 41(3): 531-539. doi: 10.11862/CJIC.20240256
3. [3]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
4. [4]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
5. [5]
  Zongpei Zhang , Yanyang Li , Yanan Si , Kai Li , Shuangquan Zang . Developing a Chemistry Experiment Center Employing a Multifaceted Approach to Serve High-Quality Laboratory Education. University Chemistry, 2024, 39(7): 13-19. doi: 10.12461/PKU.DXHX202404041
6. [6]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
7. [7]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
8. [8]
  Qiqi Li , Su Zhang , Yuting Jiang , Linna Zhu , Nannan Guo , Jing Zhang , Yutong Li , Tong Wei , Zhuangjun Fan . 前驱体机械压实制备高密度活性炭及其致密电容储能性能. Acta Physico-Chimica Sinica, 2025, 41(3): 2406009-. doi: 10.3866/PKU.WHXB202406009
9. [9]
  Yinuo Wu , Jiantao Ye , Xie Zhou , Yu Qian , Lei Guo . Teaching Design of Basic Chemistry Based on PBL Methodology for Medical Undergraduates: A Case Study on “Osmotic Pressure of Solution”. University Chemistry, 2024, 39(3): 149-157. doi: 10.3866/PKU.DXHX202309077
10. [10]
  Naiying Fan , Chuanli Qin , Guo Zhang , Bin Wang , Yan Wang , Bing Zheng , Yichun Qu , Zhiyao Sun , Guanghui An . Case Design of Course Ideological and Political Education in Chemical Experiment Safety: the Safe Use of Common Laboratory Instruments and Glassware. University Chemistry, 2024, 39(2): 242-247. doi: 10.3866/PKU.DXHX202309061
11. [11]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
12. [12]
  Yongjie ZHANG , Bintong HUANG , Yueming ZHAI . Research progress of formation mechanism and characterization techniques of protein corona on the surface of nanoparticles. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2318-2334. doi: 10.11862/CJIC.20240247
13. [13]
  Yingran Liang , Fei Wang , Jiabao Sun , Hongtao Zheng , Zhenli Zhu . Construction and Application of a New Experimental Device for Determination of Alkaline Metal Elements by Plasma Atomic Emission Spectrometry Based on Solution Cathode Glow Discharge: An Alternative Approach for Fundamental Teaching Experiments in Emission Spectroscopy. University Chemistry, 2024, 39(5): 380-387. doi: 10.3866/PKU.DXHX202312024
14. [14]
  Zeyu XU , Anlei DANG , Bihua DENG , Xiaoxin ZUO , Yu LU , Ping YANG , Wenzhu YIN . Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099
15. [15]
  Jin Tong , Shuyan Yu . Crystal Engineering for Supramolecular Chirality. University Chemistry, 2024, 39(3): 86-93. doi: 10.3866/PKU.DXHX202308113
16. [16]
  Xianfei Chen , Wentao Zhang , Haiying Du . Experimental Design of Computational Materials Science Based on Scientific Research Cases. University Chemistry, 2025, 40(3): 52-61. doi: 10.3866/PKU.DXHX202403112
17. [17]
  Jia Huo , Jia Li , Yongjun Li , Yuzhi Wang . Ideological and Political Design of Physical Chemistry Teaching: Chemical Potential of Any Component in an Ideal-Dilute Solution. University Chemistry, 2024, 39(2): 14-20. doi: 10.3866/PKU.DXHX202307075
18. [18]
  Hongwei Ma , Fang Zhang , Hui Ai , Niu Zhang , Shaochun Peng , Hui Li . Integrated Crystallographic Teaching with X-ray,TEM and STM. University Chemistry, 2024, 39(3): 5-17. doi: 10.3866/PKU.DXHX202308107
19. [19]
  Jiantao Zai , Hongjin Chen , Xiao Wei , Li Zhang , Li Ma , Xuefeng Qian . The Learning-Centered Problem-Oriented Experimental Teaching. University Chemistry, 2024, 39(4): 40-47. doi: 10.3866/PKU.DXHX202309023
20. [20]
  Yang Liu , Peng Chen , Lei Liu . Chemistry “101 Plan”: Design and Construction of Chemical Biology Textbook. University Chemistry, 2024, 39(10): 45-51. doi: 10.12461/PKU.DXHX202407085

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(364)
HTML views(73)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142