Citation: WU Shun, HAO Weiqiang, YUE Bangyi, ZHANG Peipei, DI Bin, CHEN Qiang. Influence of the distortion of gradient profile on peak width in gradient liquid chromatography[J]. Chinese Journal of Chromatography, ;2015, 33(6): 558-562. doi: 10.3724/SP.J.1123.2015.01013 shu

Influence of the distortion of gradient profile on peak width in gradient liquid chromatography

WU Shun ^1,3 ,
HAO Weiqiang ^2,3,, ,
YUE Bangyi ³ ,
ZHANG Peipei ^1,3 ,
DI Bin ⁴ ,
CHEN Qiang ^3,,

1.
1. School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China;

Corresponding author: HAO Weiqiang, CHEN Qiang,
Received Date: 20 January 2015

Fund Project: 国家自然科学基金项目(21005009,81173022) (21005009,81173022)江苏省自然科学基金项目(BK20141163) (BK20141163)烟草行业卷烟烟气重点实验室开放研究基金项目(SZBCW201300555) (SZBCW201300555)常州市应用基础研究计划项目(CJ20115002). (CJ20115002)

In gradient liquid chromatography, the gradient profile may be distorted because of the effects of solvent mixing and axial dispersion. Such effects will be significant especially when stepwise gradient and linear gradient with high slope are applied. In this work, we discussed the influence of the distortion of gradient profile on the peak width. Firstly, the chromatographic peaks were measured under linear and stepwise gradient conditions where a C18 column was used as the stationary phase, a mixture of methanol and water as the mobile phase, and biphenyl and acetophenone as the samples. Then, the response of methanol under corresponding conditions was recorded by disconnecting the column and the detection wavelength was set at 205 nm. The gradient profile at the column inlet was calculated from the methanol response profile. The theoretical values of the peak width were calculated from the set gradient profile and the gradient profile obtained at the column inlet respectively. The theoretical values were compared with the experimental ones. It is shown that the distortion of gradient profile does affect the peak width. When such influence is taken into account, the theoretical and experimental values of the peak width are well consistent.
- liquid chromatography,
- gradient elution,
- gradient profile,
- peak width
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