Citation: WU Shun, HAO Weiqiang, YUE Bangyi, ZHANG Peipei, DI Bin, CHEN Qiang. Influence of the distortion of gradient profile on peak width in gradient liquid chromatography[J]. Chinese Journal of Chromatography, ;2015, 33(6): 558-562. doi: 10.3724/SP.J.1123.2015.01013 shu

Influence of the distortion of gradient profile on peak width in gradient liquid chromatography

WU Shun ^1,3 ,
HAO Weiqiang ^2,3,, ,
YUE Bangyi ³ ,
ZHANG Peipei ^1,3 ,
DI Bin ⁴ ,
CHEN Qiang ^3,,

1.
1. School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China;

Corresponding author: HAO Weiqiang, CHEN Qiang,
Received Date: 20 January 2015

Fund Project: 国家自然科学基金项目(21005009,81173022) (21005009,81173022)江苏省自然科学基金项目(BK20141163) (BK20141163)烟草行业卷烟烟气重点实验室开放研究基金项目(SZBCW201300555) (SZBCW201300555)常州市应用基础研究计划项目(CJ20115002). (CJ20115002)

In gradient liquid chromatography, the gradient profile may be distorted because of the effects of solvent mixing and axial dispersion. Such effects will be significant especially when stepwise gradient and linear gradient with high slope are applied. In this work, we discussed the influence of the distortion of gradient profile on the peak width. Firstly, the chromatographic peaks were measured under linear and stepwise gradient conditions where a C18 column was used as the stationary phase, a mixture of methanol and water as the mobile phase, and biphenyl and acetophenone as the samples. Then, the response of methanol under corresponding conditions was recorded by disconnecting the column and the detection wavelength was set at 205 nm. The gradient profile at the column inlet was calculated from the methanol response profile. The theoretical values of the peak width were calculated from the set gradient profile and the gradient profile obtained at the column inlet respectively. The theoretical values were compared with the experimental ones. It is shown that the distortion of gradient profile does affect the peak width. When such influence is taken into account, the theoretical and experimental values of the peak width are well consistent.
- liquid chromatography,
- gradient elution,
- gradient profile,
- peak width
1. [1]
  [1] Zhu Y Z, Feng Y N, Kim J. Chinese Journal of Chromatography (朱永哲, 冯雅男, 金正汉. 色谱), 2013, 31(9): 850
2. [2]
  [2] Wiczling P, Kaliszan R. Anal Chem, 2010, 82(9): 3692
3. [3]
  [3] Zisi C, Fasoula S, Nikitas P, et al. Analyst, 2013, 138: 3771
4. [4]
  [4] Hao W Q, Zhang X M, Hou K Y. Anal Chem, 2006, 78(22): 7828
5. [5]
  [5] Hao W Q, Zhang X M, Hu F L. Anal Chem, 2007, 79(6): 2507
6. [6]
  [6] Poppe H, Paanakker J, Bronckhorst M. J Chromatogr, 1981, 204: 77
7. [7]
  [7] Gritti F, Guiochon G. J Chromatogr A, 2007, 1145: 67
8. [8]
  [8] Gritti F, Guiochon G. J Chromatogr A, 2008, 1212: 35
9. [9]
  [9] Gritti F, Guiochon G. J Chromatogr A, 2008, 1178: 79
10. [10]
  [10] Gritti F, Guiochon G. J Chromatogr A, 2009, 1216: 6124
11. [11]
  [11] Hao W Q, Di B, Chen Q, et al. J Chromatogr A, 2013, 1295: 67
12. [12]
  [12] Hao W Q, Di B, Chen Q, et al. J Chromatogr A, 2014, 1369: 191
13. [13]
  [13] Sun X L, Hao W Q, Wang J D, et al. Chinese Journal of Chromatography (孙小丽, 郝卫强, 王俊德, 等. 色谱), 2013, 31(8): 753
1. [1]
  Jingming Li , Bowen Ding , Nan Li , Nurgul . Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching. University Chemistry, 2024, 39(8): 263-269. doi: 10.3866/PKU.DXHX202312078
2. [2]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
3. [3]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
4. [4]
  Xinlong WANG , Zhenguo CHENG , Guo WANG , Xiaokuen ZHANG , Yong XIANG , Xinquan WANG . Enhancement of the fragile interface of high voltage LiCoO₂ by surface gradient permeation of trace amounts of Mg/F. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 571-580. doi: 10.11862/CJIC.20230259
5. [5]
  Borong Yu , Huijiao Zhang , Xinyu Zhang , Xiaoying Li , Shuming Chen , Zhangang Han . The Blue Elf in the Dark: Gradient Science Popularization Experiments on Chemiluminescence. University Chemistry, 2024, 39(9): 295-303. doi: 10.12461/PKU.DXHX202403107
6. [6]
  Ruilin Han , Xiaoqi Yan . Comparison of Multiple Function Methods for Fitting Surface Tension and Concentration Curves. University Chemistry, 2024, 39(7): 381-385. doi: 10.3866/PKU.DXHX202311023
7. [7]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
8. [8]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
9. [9]
  Na Li , Limin Shao . Deduction of the General Formula of the Inverse Function of the Titration Curve. University Chemistry, 2025, 40(3): 390-401. doi: 10.12461/PKU.DXHX202409134
10. [10]
  Yutong Dong , Huiling Xu , Yucheng Zhao , Zexin Zhang , Ying Wang . The Hidden World of Surface Tension and Droplets. University Chemistry, 2024, 39(6): 357-365. doi: 10.3866/PKU.DXHX202312022
11. [11]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
12. [12]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
13. [13]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
14. [14]
  Zhiwen HU , Weixia DONG , Qifu BAO , Ping LI . Low-temperature synthesis of tetragonal BaTiO₃ for piezocatalysis. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 857-866. doi: 10.11862/CJIC.20230462
15. [15]
  Chunai Dai , Yongsheng Han , Luting Yan , Zhen Li , Yingze Cao . Ideological and Political Design of Solid-liquid Contact Angle Measurement Experiment. University Chemistry, 2024, 39(2): 28-33. doi: 10.3866/PKU.DXHX202306065
16. [16]
  Feiya Cao , Qixin Wang , Pu Li , Zhirong Xing , Ziyu Song , Heng Zhang , Zhibin Zhou , Wenfang Feng . Magnesium-Ion Conducting Electrolyte Based on Grignard Reaction: Synthesis and Properties. University Chemistry, 2024, 39(3): 359-368. doi: 10.3866/PKU.DXHX202308094
17. [17]
  Haiyang Zhang , Yanzhao Dong , Haojie Li , Ruili Guo , Zhicheng Zhang , Jiangjiexing Wu . Exploring the Integration of Chemical Engineering Principle Experiment with Cutting-Edge Research Achievements. University Chemistry, 2024, 39(10): 308-313. doi: 10.12461/PKU.DXHX202405035
18. [18]
  Limin Shao , Na Li . A Unified Equation Derived from the Charge Balance Equation for Constructing Acid-Base Titration Curve and Calculating Endpoint Error. University Chemistry, 2024, 39(11): 365-373. doi: 10.3866/PKU.DXHX202401086
19. [19]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
20. [20]
  Jiahe LIU , Gan TANG , Kai CHEN , Mingda ZHANG . Effect of low-temperature electrolyte additives on low-temperature performance of lithium cobaltate batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 719-728. doi: 10.11862/CJIC.20250023

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(782)
HTML views(142)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142