Citation: GAI Qingqing, QU Feng. Preparation and adsorption characteristics of surface-imprinted polymer using bovine serum albumin and lysozyme as double template proteins[J]. Chinese Journal of Chromatography, ;2015, 33(5): 475-480. doi: 10.3724/SP.J.1123.2015.01008 shu

Preparation and adsorption characteristics of surface-imprinted polymer using bovine serum albumin and lysozyme as double template proteins

GAI Qingqing ^1,, ,
QU Feng ²

1.
1. National Institute of Clean-and-Low-Carbon Energy, Beijing 102209, China;

Corresponding author: GAI Qingqing,
Received Date: 9 January 2015

The double template surface-imprinted polymer (Bi-MIP) was synthesized by atom transfer radical polymerization (ATRP) in aqueous media, using bovine serum albumin (BSA) and lysozyme (Lyz) as the template proteins, N-isopropylacrylamide (NIPAAm) and acrylamide (AAm) as the monomers and N-[3-(dimethylamino)propyl]-methacrylamide (DMAPMA) as the assistant of basic functional monomer. The ATRP possessed the mild reaction conditions and can be initiated at room temperature without heating and ultraviolet radiations. The preparation conditions of imprinted polymer were optimized, and the content of DMAPMA as the assistant of basic functional monomer was investigated. The results showed that Bi-MIP exhibited the good adsorption capacity and selectivity in single protein solution and in mixed protein solution when the volume of DMAPMA in the preparation process of Bi-MIP was 20 μL. The rebinding capacity of Bi-MIP was evaluated according to adsorption isotherm of the imprinted polymer. The Langmuir adsorption model was employed to describe the isotherms and revealed that Bi-MIP exhibited the maximum rebinding to BSA and Lyz at 10.2 mg/g and 19.2 mg/g, respectively. And Bi-MIP had good imprinting effect and adsorption capacity to templates BSA and Lyz from egg white and bovine serum samples. This will provide a new way for specific recognition of the double/multiple target proteins in the complex biological samples.
- surface molecularly imprinted polymer,
- double template proteins,
- adsorption characteristics
1. [1]
  [1] Lv Y Q, Tan T W, Svec F. Biotechnol Adv, 2013, 31: 1172
2. [2]
  [2] Qin L, He X W, Li W Y, et al. J Chromatogr A, 2008, 1187(1/2): 94
3. [3]
  [3] Liu L J, Zheng J J, Fang G J, et al. Anal Chim Acta, 2012, 726: 85
4. [4]
  [4] Xia Y, Zhang L, Zhao E C, et al. Chinese Journal of Chromatography (夏英, 张澜, 赵尔成, 等. 色谱), 2014, 32(2): 117
5. [5]
  [5] Gai Q Q, Qu F, Liu Z J, et al. J Chromatogr A, 2010, 1217: 5035
6. [6]
  [6] Zhang Y, Guo M J, Yao J X, et al. Chinese Journal of Analytical Chemistry (张芸, 郭敏杰, 么敬霞, 等. 分析化学), 2014, 42(2): 186
7. [7]
  [7] Yuan S F, Deng Q L, Fang G Z, et al. J Chromatogr B, 2014, 960: 239
8. [8]
  [8] Gao J F, Tian H J, Wang Y, et al. Biomaterials, 2012, 33: 3344
9. [9]
  [9] LeJeune J, Spivak D A. Biosens Bioelectron, 2009, 25: 604
10. [10]
  [10] Suedee R, Srichana T, Chuchome T, et al. J Chromatogr B, 2004, 811: 191
11. [11]
  [11] Schweitz L, Andersson L, Nilsson S. Analyst, 2002, 127: 22
12. [12]
  [12] Sreenivasan K, Sivakumar R. J Appl Polym Sci, 1999, 71: 1823
13. [13]
  [13] Sreenivasan K. J Appl Polym Sci, 2001, 82: 889
14. [14]
  [14] Dickert F, Achatz P, Halikias K. Fresen J Anal Chem, 2001, 371: 11
15. [15]
  [15] Lv Y Q, Tan T W, Svec F. Biotechnol Adv, 2013, 31: 1172
16. [16]
  [16] Qin L, He X W, Zhang W, et al. J Chromatogr A, 2009, 1216(1/2): 807
17. [17]
  [17] Yu S, Luo A Q, Biswal D, et al. Talanta, 2010, 83, 156
18. [18]
  [18] Zhang W, Qin L, He X W, et al. J Chromatogr A, 2009, 1216: 4560
19. [19]
  [19] Hu C H, Chou T C. Microchem J, 2009, 91: 53
20. [20]
  [20] Kan X W, Zhao Q, Shao D L, et al. J Phys Chem B, 2010, 114: 3999
21. [21]
  [21] Kong H, Gao C, Yan D. J Am Chem Soc, 2004, 126(2): 412
22. [22]
  [22] Gai Q Q, Zhang T, Qu F, et al. J Chromatogr A, 2011, 1218: 3489
1. [1]
  Youlin SI , Shuquan SUN , Junsong YANG , Zijun BIE , Yan CHEN , Li LUO . Synthesis and adsorption properties of Zn(Ⅱ) metal-organic framework based on 3, 3', 5, 5'-tetraimidazolyl biphenyl ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1755-1762. doi: 10.11862/CJIC.20240061
2. [2]
  Junjie Zhang , Yue Wang , Qiuhan Wu , Ruquan Shen , Han Liu , Xinhua Duan . Preparation and Selective Separation of Lightweight Magnetic Molecularly Imprinted Polymers for Trace Tetracycline Detection in Milk. University Chemistry, 2024, 39(5): 251-257. doi: 10.3866/PKU.DXHX202311084
3. [3]
  Xiaosong PU , Hangkai WU , Taohong LI , Huijuan LI , Shouqing LIU , Yuanbo HUANG , Xuemei LI . Adsorption performance and removal mechanism of Cd(Ⅱ) in water by magnesium modified carbon foam. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1537-1548. doi: 10.11862/CJIC.20240030
4. [4]
  Jingke LIU , Jia CHEN , Yingchao HAN . Nano hydroxyapatite stable suspension system: Preparation and cobalt adsorption performance. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1763-1774. doi: 10.11862/CJIC.20240060
5. [5]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
6. [6]
  Ping ZHANG , Chenchen ZHAO , Xiaoyun CUI , Bing XIE , Yihan LIU , Haiyu LIN , Jiale ZHANG , Yu'nan CHEN . Preparation and adsorption-photocatalytic performance of ZnAl@layered double oxides. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1965-1974. doi: 10.11862/CJIC.20240014
7. [7]
  Shasha Ma , Zujin Yang , Jianyong Zhang . Facile Synthesis of FeBTC Metal-Organic Gel and Its Adsorption of Cr₂O₇²⁻: A Physical Chemistry Innovation Experiment. University Chemistry, 2024, 39(8): 314-323. doi: 10.3866/PKU.DXHX202401008
8. [8]
  Zhongxin YU , Wei SONG , Yang LIU , Yuxue DING , Fanhao MENG , Shuju WANG , Lixin YOU . Fluorescence sensing on chlortetracycline of a Zn-coordination polymer based on mixed ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2415-2421. doi: 10.11862/CJIC.20240304
9. [9]
  Bao Jia , Yunzhe Ke , Shiyue Sun , Dongxue Yu , Ying Liu , Shuaishuai Ding . Innovative Experimental Teaching for the Preparation and Modification of Conductive Organic Polymer Thin Films in Undergraduate Courses. University Chemistry, 2024, 39(10): 271-282. doi: 10.12461/PKU.DXHX202404121
10. [10]
  Xiao SANG , Qi LIU , Jianping LANG . Synthesis, structure, and fluorescence properties of Zn(Ⅱ) coordination polymers containing tetra-alkenylpyridine ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2124-2132. doi: 10.11862/CJIC.20240158
11. [11]
  Xingchao Zhao , Xiaoming Li , Ming Liu , Zijin Zhao , Kaixuan Yang , Pengtian Liu , Haolan Zhang , Jintai Li , Xiaoling Ma , Qi Yao , Yanming Sun , Fujun Zhang . 倍增型全聚合物光电探测器及其在光电容积描记传感器上的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2311021-. doi: 10.3866/PKU.WHXB202311021
12. [12]
  Jiali CHEN , Guoxiang ZHAO , Yayu YAN , Wanting XIA , Qiaohong LI , Jian ZHANG . Machine learning exploring the adsorption of electronic gases on zeolite molecular sieves. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 155-164. doi: 10.11862/CJIC.20240408
13. [13]
  You Wu , Chang Cheng , Kezhen Qi , Bei Cheng , Jianjun Zhang , Jiaguo Yu , Liuyang Zhang . ZnO/D-A共轭聚合物S型异质结高效光催化产H₂O₂及其电荷转移动力学研究. Acta Physico-Chimica Sinica, 2024, 40(11): 2406027-. doi: 10.3866/PKU.WHXB202406027
14. [14]
  .
  南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积
  . CCS Chemistry, 2025, 7(0): -.
15. [15]
  Yongjie ZHANG , Bintong HUANG , Yueming ZHAI . Research progress of formation mechanism and characterization techniques of protein corona on the surface of nanoparticles. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2318-2334. doi: 10.11862/CJIC.20240247
16. [16]
  Qianqian Zhong , Yucui Hao , Guotao Yu , Lijuan Zhao , Jingfu Wang , Jian Liu , Xiaohua Ren . Comprehensive Experimental Design for the Preparation of the Magnetic Adsorbent Based on Enteromorpha Prolifera and Its Utilization in the Purification of Heavy Metal Ions Wastewater. University Chemistry, 2024, 39(8): 184-190. doi: 10.3866/PKU.DXHX202312013
17. [17]
  Tingyu Zhu , Hui Zhang , Wenwei Zhang . Exploration and Practice of Ideological and Political Education in the Course of Experiments on Chemical Functional Molecules: Synthesis and Catalytic Performance Study of Chiral Mn(III)Cl-Salen Complex. University Chemistry, 2024, 39(4): 75-80. doi: 10.3866/PKU.DXHX202311011
18. [18]
  Xinyu Yin , Haiyang Shi , Yu Wang , Xuefei Wang , Ping Wang , Huogen Yu . Spontaneously Improved Adsorption of H₂O and Its Intermediates on Electron-Deficient Mn^(3+δ)+ for Efficient Photocatalytic H₂O₂ Production. Acta Physico-Chimica Sinica, 2024, 40(10): 2312007-. doi: 10.3866/PKU.WHXB202312007
19. [19]
  Weihan Zhang , Menglu Wang , Ankang Jia , Wei Deng , Shuxing Bai . 表面硫物种对钯-硫纳米片加氢性能的影响. Acta Physico-Chimica Sinica, 2024, 40(11): 2309043-. doi: 10.3866/PKU.WHXB202309043
20. [20]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(286)
HTML views(31)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Preparation and adsorption characteristics of surface-imprinted polymer using bovine serum albumin and lysozyme as double template proteins

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

Metrics

通讯作者: 陈斌, bchen63@163.com