Citation: HOU Yujie, ZHU Wenjun, CHEN Changgong, WANG Yan, DUAN Zhijun, YAN Chao. Exploration on serum metabolic biomarkers of hepatitis B virus infected patients based on gas chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(4): 383-388. doi: 10.3724/SP.J.1123.2014.12007 shu

Exploration on serum metabolic biomarkers of hepatitis B virus infected patients based on gas chromatography-mass spectrometry

HOU Yujie ¹ ,
ZHU Wenjun ² ,
CHEN Changgong ¹ ,
WANG Yan ^1,, ,
DUAN Zhijun ^3,, ,
YAN Chao ^1,,

1.
1. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China;

Corresponding author: WANG Yan, DUAN Zhijun, YAN Chao,
Received Date: 3 December 2014
Available Online: 5 January 2015
Fund Project: 国家自然科学基金项目(21175092,21105064) (21175092,21105064)国家重大科学仪器设备开发专项(2011YQ150072,2011YQ15007204,2011YQ15007207,2011YQ15007210) (2011YQ150072,2011YQ15007204,2011YQ15007207,2011YQ15007210)上海市自然科学基金项目(12ZR1413600). (12ZR1413600)

The difference of serum metabolome between hepatitis B virus (HBV) infected patients and healthy controls was explored for the potential metabolite biomarkers of HBV disease using serum metabolomics approach. Totally 30 HBV infected patients and 35 healthy controls were enrolled. Gas chromatography-time-of-flight mass spectrometry (GC-TOFMS), pattern recognition by principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were applied in each group. Several metabolites which were different between the two groups based on variable importance in projection (VIP) value, non-parametric test and screening in databases were identified. Ten variables that were significantly different were considered as the potential biomarkers, among which five variables (citric acid, aconitic acid, glutamine, N,N-dimethylglycine and malonic acid) showed good correlation with HBV patients. The area under the receiver operating characteristic curve was 0.975, with good specificity and sensitivity. A panel of metabolite markers composed of citric acid, aconitic acid, glutamine, N,N-dimethylglycine and malonic acid from GC-TOFMS were selected to discriminate HBV subjects from their healthy counterparts. These biochemical changes provide a novel molecular diagnostic approach which could be helpful to further understand the pathogenesis and identify the therapeutic target of HBV disease.
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