Citation: LIN Chunhua, XIE Xianqing, FAN Naili, TU Yuanhong, CHEN Yan, LIAO Weilin. Fast analysis of common fatty acids in edible vegetable oils by ultra-performance convergence chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(4): 397-402. doi: 10.3724/SP.J.1123.2014.12004 shu

Fast analysis of common fatty acids in edible vegetable oils by ultra-performance convergence chromatography-mass spectrometry

1.
National Research Center of Monosaccharide Chemical Synthesis Engineering, Jiangxi Normal University, Nanchang 330027, China

Corresponding author: LIAO Weilin,
Received Date: 2 December 2014
Available Online: 8 January 2015
Fund Project: 国家科技支撑计划课题(2014BAE13B02,2012BAE07B00). (2014BAE13B02,2012BAE07B00)

A fast analytical method for five common fatty acids in six edible vegetable oils was developed by ultra-performance convergence chromatography-mass spectrometry (UPC²-MS). The five fatty acids are palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid. Their contents in the corn oil, sunflower oil, soybean oil, tea oil, rapeseed oil and peanut oil were compared. The chromatographic separation was performed on an ACQUITY UPC² BEH 2-EP column (100 mm×2.1 mm, 1.7 μm) using the mobile phases of carbon dioxide and methanol/acetonitrile (1:1, v/v) with gradient elution. The separated compounds were detected by negative electrospray ionization ESI^--MS. The results showed that the reasonable linearities were achieved for all the analytes over the range of 0.5-100 mg/L with the correlation coefficients (R²) of 0.9985-0.9998. The limits of quantification(S/N≥10)of the five fatty acids were 0.15-0.50 mg/L. The recoveries of the five fatty acids at three spiked levels were in the range of 89.61%-108.50% with relative standard deviations of 0.69%-3.01%. The developed method showed high performance, good resolution and fast analysis for the underivatized fatty acids. It has been successfully used to detect the five fatty acids from corn oil, sunflower oil, soybean oil, tea oil, rapeseed oil and peanut oil.
- ultra-performance convergence chromatography-mass spectrometry(UPC²-MS),
- fatty acids,
- edible vegetable oils,
- underivatization
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沈阳化工大学材料科学与工程学院沈阳 110142