Citation: LIN Shuhai, CAI Zongwei. Isotopic tracing technique in quantitative analysis of metabolic reprogramming in tumor cells[J]. Chinese Journal of Chromatography, ;2015, 33(2): 112-115. doi: 10.3724/SP.J.1123.2014.11011 shu

Isotopic tracing technique in quantitative analysis of metabolic reprogramming in tumor cells

LIN Shuhai ^, ,
CAI Zongwei ^,

1.
Department of Chemistry, Hong Kong Baptist University, State Key Laboratory of Environmental and Biological Analysis, Hong Kong 999077, China

Corresponding author: LIN Shuhai, CAI Zongwei,
Received Date: 10 November 2014
Available Online: 9 December 2014
Fund Project: 国家自然科学基金资助项目(21377106). (21377106)

It is hard to begin to discuss tumor metabolism without mentioning Warburg effect. Tumor metabolism has been received intensive attention in recent years for understanding Warburg effect which is also known as aerobic glycolysis. Even Warburg did not anticipate, other nutrients, such as glutamine, serine and glycine could serve as alternative source for energy demands and block building in cancer cells. To delineate altered metabolic pathways in cancer cells, isotopic tracing technique provides a novel and holistic insight in metabolic switch under control of oncogenes and tumor suppressor genes. An array of chromatography coupled with mass spectrometry is the powerful tool to detect the isotope-labeled metabolites for unlocking the underlying biochemical reactions in cancer cells. In this review article, we focus on carbon-13 and deuterium-labeling for quantitative analysis of intermediate metabolites including reduced form of nicotinamide adenine dinucleotide phosphate (NADPH). NADPH is mainly derived from pentose phosphate pathway in previous biochemical textbook. However, deuterium-isotope labeling metabolic flux analysis can reveal other important pathways for NADPH production, such as one-carbon metabolism, which is also discussed in this review.
- metabolic flux analysis,
- isotopic tracing,
- cellular metabolism,
- mass spectrometry (MS),
- review
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