Citation: OU Wanlu, LI Yujuan, SHI Dongdong, QU Feng. Determination of gambogic acid in Gamboge by non-aqueous capillary electrophoresis[J]. Chinese Journal of Chromatography, ;2015, 33(2): 152-157. doi: 10.3724/SP.J.1123.2014.11006 shu

Determination of gambogic acid in Gamboge by non-aqueous capillary electrophoresis

OU Wanlu ¹ ,
LI Yujuan ¹ ,
SHI Dongdong ² ,
QU Feng ^1,,

1.
1. School of Life Science, Beijing Institute of Technology, Beijing 100081, China;

Corresponding author: QU Feng,
Received Date: 5 November 2014
Available Online: 18 December 2014
Fund Project: 国家重点基础研究发展计划项目("973"项目)(2012CB910603) ("973"项目)(2012CB910603)国家自然科学基金项目(21175011,21375008,81202996). (21175011,21375008,81202996)

Gambogic acid (GA), a kind of caged xanthones, has low solubility in water. A non-aqueous capillary electrophoresis (NACE) was established for the determination of GA in Gamboge based on the optimized conditions. The effect of 20%-60% methanol or acetonitrile spiked in running solution was investigated. The effects of compositions, concentration, pH, additives like β-cyclodextrin in running buffer were thoroughly studied. Applied voltage and applied temperature were also observed. Optimal electrophoretic conditions were as follows: 20 mmol/L sodium borohydride solution (pH 9.86) containing 40% (v/v) acetonitrile, 10 mmol/L β-cyclodextrin as running buffer, applied voltage of 10 kV, capillary temperature of 30 ℃ and detection wavelength of 280 nm. The calibration curve had good linearity in the range of 2-2000 mg/L with the correlation coefficient of 0.9996. The limit of quantification (S/N=3) of the method was 2 mg/L. The quantifications of GA in Gamboge from different producing places including Vietnam, Thailand, Burma, India were 1.67-472.40 mg/g with the RSD (n=3) of 1.12%-2.60%. The content of Gamboge from Vietnam is obviously low while the others are high. The recoveries of GA spiked in real samples ranged from 95.2% to 105.6%. The method of NACE is simple, efficient and of good reproducibility, can be served as a novel reference to identify and control the quality of Gamboge.
- non-aqueous capillary electrophoresis (NACE),
- gambogic acid,
- Gamboge
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