Citation: ZHOU Xiao, LI Xiaolan, CHEN Zhiyan, YE Changwen, ZHOU Yun, MENG Dongling. Determination of three isothiazolinone biocides in water-borne adhesives by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2015, 33(1): 75-79. doi: 10.3724/SP.J.1123.2014.10012 shu

Determination of three isothiazolinone biocides in water-borne adhesives by high performance liquid chromatography

1.
1. China Tobacco Guangxi Industrial Limited Liability Company, Nanning 530001, China;

Corresponding author: ZHOU Xiao,
Received Date: 20 October 2014
Available Online: 7 November 2014

A rapid high performance liquid chromatography (HPLC) method was developed for the quantitative analysis of three isothiazolinone biocides (2-methyl-4-isothiazolin-3-one (MI), 5-chloro-2-methyl-4-isothiazolin-3-one (CMI) and 1,2-benzylisothiazolin-3-one (BIT)) in water-borne adhesives. The sample was extracted with methanol-water (1: 1, v/v), and purified by centrifugation and filtration. The isothiazolones were separated on a C18 column with methanol-water as mobile phases under gradient elution and detected with a diode array detector (DAD). The pretreatment factors such as extraction solvent, extraction method, dilution ratio, extraction time were optimized. Under the optimized conditions, the targets had good linearities (r²≥0.9992) in the range of 0.25-10.0 mg/L. The recoveries were between 92% and 103% with the relative standard deviations not more than 4%. The limits of detection (LODs) were between 0.43 mg/kg and 1.14 mg/kg. The limits of quantification (LOQs) were between 1.44 mg/kg and 3.81 mg/kg. The results showed that the method can achieve the purpose of quantitative detection. The analyses of real samples verified the reliability of this method.
- high performance liquid chromatography (HPLC),
- isothiazolone biocides,
- water-borne adhesives
1. [1]
  [1] Chen Y C, Xie X B, Shi Q S, et al. Fine and Specialty Chemicals (陈艺彩, 谢小保, 施庆珊, 等. 精细与专用化学品), 2010, 18(1): 43
2. [2]
  [2] Yang R J, Wei B W, Yu W J, et al. Chinese Journal of Chromatography (杨荣静, 卫碧文, 于文佳, 等. 色谱), 2011, 29(6): 513
3. [3]
  [3] Lin F, Mao S L, Chen B, et al. China Leather (林芳, 毛树禄, 陈斌, 等. 中国皮革), 2011, 40(7): 5
4. [4]
  [4] Jian L H, Wen H L, Sun J, et al. Chinese Journal of Health Laboratory Technology (简龙海, 闻宏亮, 孙健, 等. 中国卫生检验杂志), 2011, 21(11): 2652
5. [5]
  [5] Ministry of Health of the People's Republic of China. Hygienic Standard for Cosmetics. Beijing: Chinese Health Publishing House (中华人民共和国卫生部. 化妆品卫生规范. 北京: 中国卫生出版社), 2007: 234
6. [6]
  [6] Feng L, Ying Y, Tan Y, et al. Chinese Journal of Health Laboratory (冯靓, 应英, 谭莹, 等. 中国卫生检验杂志), 2010, 20(9): 2171
7. [7]
  [7] Lin Q B, Wang T J, Song H, et al. Food Additives & Contaminants: Part A, 2010, 27(12): 1775
8. [8]
  [8] Wang T J. [MS Dissertation]. Taiyuan: Shanxi University (王天娇. [硕士学位论文]. 太原: 山西大学), 2011: 35
9. [9]
  [9] BS EN 71-11: 2005 Safety of Toys: 41
10. [10]
  [10] Zhang J M, Pan Y N, Bai H. Industrial Water Treatment (张建枚, 潘亚男, 白桦. 工业水处理), 2011, 31(5): 70
11. [11]
  [11] Willingham G L. America Patent, 5094957. [1992-03-10]
12. [12]
  [12] Geyer W G. America Patent, 3975155. [1976-08-17]
13. [13]
  [13] Chen Z F, Ying G G, Lai H J, et al. Anal Bioanal Chem, 2012, 404(10): 3175
14. [14]
  [14] Wu T, Wang C, Wang X, et al. Chinese Journal of Analytical Chemistry (武婷, 王超, 王星, 等. 分析化学), 2007, 35(10): 1439
15. [15]
  [15] Li J, Zhao J H. Chinese Journal of Health Laboratory Technology (李洁, 赵金辉. 中国卫生检验杂志), 2000, 10(5): 520
16. [16]
  [16] HG/T 3657-2008
17. [17]
  [17] Gerardo A R, Thierry D, Marta L, et al. J Chromatogr A, 2012, 1270: 42
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沈阳化工大学材料科学与工程学院沈阳 110142