Citation: CHENG Quan, YANG Fang, LI Jie, LU Shengyu, LAN Jinchang, JIANG Jinbin. Analysis of the volatile components in Minnan oolong tea by headspace solid phase microextraction coupled with comprehensive two-dimensional gas chromatography- time of flight mass spectrometry and the application in its variety identification[J]. Chinese Journal of Chromatography, ;2015, 33(2): 174-181. doi: 10.3724/SP.J.1123.2014.09042 shu

Analysis of the volatile components in Minnan oolong tea by headspace solid phase microextraction coupled with comprehensive two-dimensional gas chromatography- time of flight mass spectrometry and the application in its variety identification

1.
1. Inspection and Quarantine Technical Center of Fujian Entry-Exit Inspection & Quarantine Bureau, Key Laboratory of Inspection and Quarantine of Fujian, Fuzhou 350001, China;

Received Date: 25 September 2014
Available Online: 30 October 2014
Fund Project: 国家质检总局科研项目(2013IK147) (2013IK147)福建省科技重点项目(2012Y6001). (2012Y6001)

A method to analyze the volatile components in Minnan oolong tea was developed based on headspace solid phase microextraction (HS-SPME) coupled with comprehensive two-dimensional gas chromatography-time of flight mass spectrometry (GC×GC-TOF MS). Volatile compounds of 48 oolong tea samples with different qualities and production seasons from five varieties (Tieguanyin, Huangjingui, Benshan, Maoxie and Meizhan) were extracted by HS-SPME and analyzed by GC×GC-TOF MS. More than 2000 peaks were obtained from each sample, and 51 common compounds were tentatively identified by comparison with the standard mass spectrum databases, retention indices and structure spectra. The projection score of the common compounds obtained from principal component analysis (PCA) had presented a straightforward classification trend for different oolong tea varieties. In addition, 9 compounds which had significant impact on the classification were selected by stepwise discriminate analysis, and used as variables to establish four discriminated functions by Fisher's discriminate analysis (FDA). The accuracy for the recognition of 48 samples was 97.9%. The results had demonstrated the feasibility of the method to be used to discriminate the oolong tea varieties.
1. [1]
  [1] Seetohul L N, Islam M, Hare W, et al. J Sci Food Agric, 2006, 86: 2092
2. [2]
  [2] Tanaka T, Kouno I. Food Sci Technol Res, 2003, 9: 128
3. [3]
  [3] Cheng Q, Yang F, Wang D H, et al. Spectroscopy and Spectral Analysis (程权, 杨方, 王丹红, 等. 光谱学与光谱分析), 2014, 34(3): 656
4. [4]
  [4] Huo D Q, Wu Y, Yang M, et al. Food Chem, 2014, 145: 639
5. [5]
  [5] Wang L F, Lee J Y, Chung J O, et al. Food Chem, 2008, 109: 196
6. [6]
  [6] Ye N S, Zhang L Q, Gu X X. Food Anal Methods, 2012, 5: 856
7. [7]
  [7] Lin J, Zhang P, Pan Z Q, et al. Food Chem, 2013, 141: 259
8. [8]
  [8] Zhao F, Liu J, Wang X, et al. Eur J Lipid Sci Technol, 2013, 115(3): 337
9. [9]
  [9] Vestner J, Malherbe S, Du Toit M, et al. J Agric Food Chem, 2011, 59(24): 12732
10. [10]
  [10] Villière A, Arvisenet G, Lethuaut L, et al. Food Chem, 2012, 131(4): 1561
11. [11]
  [11] Spanik I, Janacova A, Susterova Z, et al. Chem Pap, 2013, 67(2): 127
12. [12]
  [12] Zhang L, Zeng Z D, Ye G Z, et al. Chinese Journal of Chromatography (张磊, 曾仲大, 叶国柱, 等. 色谱), 2014, 32(8): 804
13. [13]
  [13] Yuan H B, Yin J F, Ye G Z, et al. China Tea (袁海波, 尹军峰, 叶国柱, 等. 中国茶叶), 2009(9): 14
1. [1]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
2. [2]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
3. [3]
  Lijun Dong , Pengcheng Du , Guangnong Lu , Wei Wang . Exploration and Practice of Independent Design Experiments in Inorganic and Analytical Chemistry: A Case Study of “Preparation and Composition Analysis of Tetraammine Copper(II) Sulfate”. University Chemistry, 2024, 39(4): 361-366. doi: 10.3866/PKU.DXHX202310041
4. [4]
  Yuan Zheng , Quan Lan , Zhenggen Zha , Lingling Li , Jun Jiang , Pingping Zhu . Teaching Reform of Organic Synthesis Experiments by Introducing Reverse Thinking and Design Concepts: Taking the Synthesis of Cinnamic Acid Based on Retrosynthetic Analysis as an Example. University Chemistry, 2024, 39(6): 207-213. doi: 10.3866/PKU.DXHX202310065
5. [5]
  Fang Niu , Rong Li , Qiaolan Zhang . Analysis of Gas-Solid Adsorption Behavior in Resistive Gas Sensing Process. University Chemistry, 2024, 39(8): 142-148. doi: 10.3866/PKU.DXHX202311102
6. [6]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
7. [7]
  Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120
8. [8]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
9. [9]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
10. [10]
  Jingming Li , Bowen Ding , Nan Li , Nurgul . Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching. University Chemistry, 2024, 39(8): 263-269. doi: 10.3866/PKU.DXHX202312078
11. [11]
  Tingting Yu , Si Chen , Lianglong Sun , Tongtong Shi , Kai Sun , Xin Wang . Comprehensive Experimental Design for the Photochemical Synthesis, Analysis, and Characterization of Difluoropyrroles. University Chemistry, 2024, 39(11): 196-203. doi: 10.3866/PKU.DXHX202401022
12. [12]
  Gaoyan Chen , Chaoyue Wang , Juanjuan Gao , Junke Wang , Yingxiao Zong , Kin Shing Chan . Heart to Heart: Exploring Cardiac CT. University Chemistry, 2024, 39(9): 146-150. doi: 10.12461/PKU.DXHX202402011
13. [13]
  Zhuomin Zhang , Hanbing Huang , Liangqiu Lin , Jingsong Liu , Gongke Li . Course Construction of Instrumental Analysis Experiment: Surface-Enhanced Raman Spectroscopy for Rapid Detection of Edible Pigments. University Chemistry, 2024, 39(2): 133-139. doi: 10.3866/PKU.DXHX202308034
14. [14]
  Zhenli Sun , Ning Wang , Kexin Lin , Qin Dai , Yufei Zhou , Dandan Cao , Yanfeng Dang . Visual Analysis of Hotspots and Development Trends in Analytical Chemistry Education Reform. University Chemistry, 2024, 39(11): 57-64. doi: 10.12461/PKU.DXHX202403095
15. [15]
  Zhaoyang Li , Haiyan Zhao , Yali Zhang , Yuan Zhang , Shiqiang Cui . Integration of Nobel Prize Achievements in Analytical Technology with College Instrumental Analysis Course. University Chemistry, 2025, 40(3): 269-276. doi: 10.12461/PKU.DXHX202405131
16. [16]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
17. [17]
  Zhening Lou , Quanxing Mao , Xiaogeng Feng , Lei Zhang , Xu Xu , Yuyang Zhang , Xueyan Liu , Hongling Kang , Dongyang Feng , Yongku Li . Practice of Implementing Blended Teaching in Shared Analytical Chemistry Course. University Chemistry, 2024, 39(2): 263-269. doi: 10.3866/PKU.DXHX202308089
18. [18]
  Yan Zhang , Ping Wang , Tiebo Xiao , Futing Zi , Yunlong Chen . Measures for Ideological and Political Construction in Analytical Chemistry Curriculum. University Chemistry, 2024, 39(4): 255-260. doi: 10.3866/PKU.DXHX202401017
19. [19]
  Xiaofei Zhou , Yu-Qing Cao , Feng Zhu , Li Qi , Linhai Liu , Ni Yan , Zhiqiang Zhu . Missions and Challenges of Instrumental Analysis Course in the New Era. University Chemistry, 2024, 39(6): 174-180. doi: 10.3866/PKU.DXHX202310058
20. [20]
  . . Chinese Journal of Inorganic Chemistry, 2024, 40(12): 0-0.

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(316)
HTML views(35)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142