Citation: WU Lidong, LIU Huan, LI Jincheng, FU Xiaochen, SONG Yi. Determination of phenol in water by electrochemical tyrosinase biosensor based on ordered graphitized mesoporous carbon and evaluated by high performance liquid chromatography[J]. Chinese Journal of Chromatography, ;2014, 32(12): 1368-1372. doi: 10.3724/SP.J.1123.2014.09008 shu

Determination of phenol in water by electrochemical tyrosinase biosensor based on ordered graphitized mesoporous carbon and evaluated by high performance liquid chromatography

1.
Quality and Standard Research Center, Chinese Academy of Fishery Sciences, Beijing 100141, China

Corresponding author: SONG Yi,
Received Date: 4 September 2014

Fund Project: 国家自然科学基金项目(21307161) (21307161)中央级公益性科研院所基本科研业务费专项资金项目(2013C006). (2013C006)

A novel electrochemical tyrosinase biosensor based on ordered graphitized mesoporous carbon (GMC) was obtained, which was used as a platform for phenol detection. The accuracy of tyrosinase biosensor method was comparatively evaluated by high performance liquid chromatography (HPLC). By entrapping tyrosinase molecules (6.5 nm×9.8 nm×5.5 nm) into the mesopores of GMC (diameter 10 nm, GMC10), the "interspace confinement effect" of GMC10 may improve the stability of tyrosinase in vitro. After 21-day storage, the GMC10-based tyrosinase biosensor retained more than 85% of its initial response. It is indicated that GMC10 with "interspace confinement effect" can significantly prolong the life of tyrosinase molecules in vitro. Furthermore, the GMC-based tyrosinase biosensor displayed excellent analytical performances for phenol detection, such as stability, repeatability, selectivity, sensitivity and limit of detection. The GMC-based tyrosinase biosensor demonstrated a linear response for phenol from 0.1 to 10 μmol/L with a low detection limit of 20 nmol/L. The comparative study between HPLC and GMC-based tyrosinase biosensor showed that the detection of phenol in water sample by the GMC-based tyrosinase biosensor method is reliable, accurate and effective. The proposed GMC-based tyrosinase biosensor proved to be a very promising "pre-alarm" tool for rapid detecting phenol pollution in emergency accidents.
- high performance liquid chromatography (HPLC),
- electrochemical biosensor,
- graphitized mesoporous carbon (GMC),
- tyrosinase,
- phenol,
- water
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沈阳化工大学材料科学与工程学院沈阳 110142