Citation: LIU Sisi, DU Juan, CHEN Jingwen, ZHAO Hongxia. Determination of 19 antibiotic and 2 sulfonamide metabolite residues in wild fish muscle in mariculture areas of Laizhou Bay using accelerated solvent extraction and high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(12): 1320-1325. doi: 10.3724/SP.J.1123.2014.08032 shu

Determination of 19 antibiotic and 2 sulfonamide metabolite residues in wild fish muscle in mariculture areas of Laizhou Bay using accelerated solvent extraction and high performance liquid chromatography-tandem mass spectrometry

1.
Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science & Technology, Dalian University of Technology, Dalian 116024, China

Corresponding author: ZHAO Hongxia,
Received Date: 28 August 2014
Available Online: 23 September 2014
Fund Project: 国家"973"重点基础研究发展计划项目(2013CB430403) (2013CB430403)国家自然科学基金项目(21277017). (21277017)

A sample preparation and analytical method with accelerated solvent extraction (ASE) and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed to detect 19 antibiotic (9 sulfonamides, 4 quinolones, 3 macrolides and 3 others) and 2 sulfonamide metabolite residues in fish muscle. The target compounds were extracted using ASE and purified simultaneously by a C18 resin in the extraction cell. The extracts were evaporated to dryness, and redissolved with the initial mobile phase for HPLC-MS/MS analysis after freezing centrifugation (10000 r/min, -4 ℃) to remove the fat and other matrix compounds further. The separation of the analytes was carried out on an Xterra MS C18 column with methanol-acetonitrile (1:1, v/v) as mobile phase A and 0.1% formic acid (containing 0.1% ammonium formate) as mobile phase B. The spiked recoveries of the method were 55.2%-113.3%, with the relative standard deviations of 0.1%-17.6%(n=6). The limits of detection ranged from 0.003 to 0.6 ng/g. The method was applied to two fish (Synechogobius hasta and Liza haematocheilus) collected in mariculture areas of Laizhou Bay and six antibiotics were detected, in which the mass concentrations of norfloxacin were highest with mean values of 67.01 and 27.58 ng/g, respectively. The method is simple, rapid, highly sensitive, and useful in the study on exposure levels and environmental behavior of the antibiotics.
- accelerated solvent extraction (ASE),
- high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),
- antibiotics,
- sulfonamide metabolites,
- residues,
- fish,
- mariculture
1. [1]
  [1] Li Z X, Tian B K, Zuo J E, et al. Environmental Engineering (李再兴, 田宝阔, 左剑恶, 等. 环境工程), 2012, 30(2): 72
2. [2]
  [2] Food and Agriculture Organization of the United Nations (FAO). The State of World Fisheries and Aquaculture 2012. Rome: FAO Fisheries and Aquaculture Department, 2012: 27
3. [3]
  [3] Wang D, Sui Q, Zhao W T, et al. Chinese Science Bulletin (王丹, 隋倩, 赵文涛, 等. 科学通报), 2014, 59(9): 743
4. [4]
  [4] Gao P P, Mao D Q, Luo Y, et al. Water Res, 2012, 46(7): 2355
5. [5]
  [5] Willmott A, McCombie A, Rossleigh M. Intern Med J, 2011, 41(Suppl 3): 25
6. [6]
  [6] Sun W H, Leng K L, Wang Z J, et al. Food Science (孙伟红, 冷凯良, 王志杰, 等. 食品科学), 2009, 30(24): 294
7. [7]
  [7] Zhou Q, Peng D P, Wang Y L, et al. Food Chem, 2014, 154: 52
8. [8]
  [8] Yu H, Tao Y F, Chen D M, et al. J Chromatogr B, 2012, 885: 150
9. [9]
  [9] Tao Y F, Yu G, Chen D M, et al. J Chromatogr B, 2012, 897: 64
10. [10]
  [10] Gentili A, Perret D, Marchese S, et al. J Agric Food Chem, 2004, 52(15): 4614
11. [11]
  [11] Yang C Q, Wang L X, Hou X H, et al. Chinese Journal of Chromatography (杨常青, 王龙星, 侯晓虹, 等. 色谱), 2012, 30(8): 756
12. [12]
  [12] Meng J, Yang Y H. Chinese Journal of Food Hygiene (孟娟, 杨永红. 中国食品卫生杂志), 2012, 24(6): 546
13. [13]
  [13] Gong Q, Ding L, Zhu S H, et al. Chinese Journal of Chromatography (龚强, 丁利, 朱绍华, 等. 色谱), 2012, 30(11): 1143
14. [14]
  [14] Ding Y J, Zhang W H, Gu C, et al. J Chromatogr A, 2011, 1218(1): 10
15. [15]
  [15] Li W H, Shi Y L, Gao L H, et al. Journal of Instrumental Analysis (厉文辉, 史亚利, 高立红, 等. 分析测试学报), 2010, 29(10): 987
16. [16]
  [16] Zhao H, Meng X Z, Xiang N, et al. Environmental Chemistry (赵恒, 孟祥周, 向楠, 等. 环境化学), 2012, 31(5): 573
17. [17]
  [17] Zhu L Y, Hites R A. Environ Sci Technol, 2006, 40(12): 3711
18. [18]
  [18] IUPAC. Pure Appl Chem, 1976, 45(2): 107
19. [19]
  [19] Runnqvist H, Bak S A, Hansen M, et al. J Chromatogr A, 2010, 1217(16): 2447
20. [20]
  [20] Carretero V, Blasco C, Picó Y. J Chromatogr A, 2008, 1209(1/2): 162
21. [21]
  [21] Pecorelli I, Galarini R, Bibi R, et al. Anal Chim Acta, 2003, 483(1/2): 81
22. [22]
  [22] Na G S, Fang X D, Cai Y Q, et al. Mar Pollut Bull, 2013, 69(1/2): 233
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