Citation: LIANG Tu, FU Qing, XIN Huaxia, LI Fangbing, JIN Yu, LIANG Xinmiao. Structural characterization of Astragalus polysaccharides using partial acid hydrolysis-hydrophilic interaction liquid chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(12): 1306-1312. doi: 10.3724/SP.J.1123.2014.08023 shu

Structural characterization of Astragalus polysaccharides using partial acid hydrolysis-hydrophilic interaction liquid chromatography-mass spectrometry

LIANG Tu ¹ ,
FU Qing ¹ ,
XIN Huaxia ¹ ,
LI Fangbing ¹ ,
JIN Yu ¹ ,
LIANG Xinmiao ^1,2,,

1.
1. School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;

Corresponding author: LIANG Xinmiao,
Received Date: 25 August 2014
Available Online: 10 October 2014
Fund Project: 高等学校博士学科点专项科研基金项目(20130074120017) (20130074120017)中国博士后科学基金面上项目(2013M541481). (2013M541481)

Water-soluble polysaccharides from traditional Chinese medicine (TCM) have properties of broad-spectrum treatment and low toxicity, making them as important components in natural medicines and health products. In order to solve the problem of polysaccharides characterization caused by their complex structures, a "bottom-up" approach was developed to complete the characterization of polysaccharides from Astragalus. Firstly, Astragalus pieces were extracted with hot water and then were precipitated by ethanol to obtain Astragalus polysaccharides. Secondly, a partial acid hydrolysis method was carried out and the effects of time, acid concentration and temperature on hydrolysis were investigated. The degree of hydrolysis increased along with the increase of hydrolysis time and acid concentration. The temperature played a great role in the hydrolysis process. No hydrolysis of the polysaccharides occurred at low temperature, while the polysaccharides were almost hydrolyzed to monosaccharide at high temperature. Under the optimum hydrolysis conditions (4 h, 1.5 mol/L trifluoroacetic acid, and 80 ℃), Astragalus polysaccharides were hydrolyzed to characteristic oligosaccharide fragments. At last, a hydrophilic liquid chromatography-mass spectrometry method was used for the separation and structural characterization of the polysaccharide hydrolysates. The results showed that the resulting polysaccharides were mainly 1→4 linear glucan, and gluco-oligosaccharides with the degrees of polymerization (DP) of 4-11 were obtained after partial acid hydrolysis. The significance of this study is that it is the guidance for the characterization of other TCM polysaccharides.
- partial acid hydrolysis,
- hydrophilic interaction liquid chromatography (HILIC),
- mass spectrometry (MS),
- polysaccharides,
- Astragalus,
- characterization
1. [1]
  [1] Zhang B, Lin R C, Feng F. Chinese Pharmaceutical Affairs (张彬, 林瑞超, 冯芳. 中国药事), 2004, 18(9): 566
2. [2]
  [2] Deng G Q, Su Z N, Zhang J R, et al. Medical Information (邓桂球, 苏振宁, 张健润, 等. 医学信息), 2014, 27(7): 48
3. [3]
  [3] Zeng L N, Yuan Y H, Huang S Y, et al. Fujian Forestry (曾丽娜, 袁玉虹, 黄淑燕, 等. 福建林业), 2014(2): 25
4. [4]
  [4] Xue M, Yang J Y, Dan S Y, et al. Journal of Mountain Agriculture and Biology (薛敏, 杨继勇, 但仕勇, 等. 山地农业生物学报), 2014, 33(2): 14
5. [5]
  [5] Hu M H, Ma F L, Science and Technology Innovation Herald (胡明华, 马方励. 科技创新导报), 2010(30): 10
6. [6]
  [6] Reis A, Domingues M R M, Ferrer-Correia A J, et al. Carbohydr Polym, 2003, 53(1): 101
7. [7]
  [7] Daniel R, Chevolot L, Carrascal M, et al. Carbohydr Res, 2007, 342(6): 826
8. [8]
  [8] Akpinar O, Erdogan K, Bakir U, et al. LWT-Food Sci Technol, 2010, 43(1): 119
9. [9]
  [9] Yan X, Evenocheck H M. Carbohydr Polym, 2012, 87(2): 1774
10. [10]
  [10] Akpinar O, Erdogan K, Bostanci S. Carbohydr Res, 2009, 344(5): 660
11. [11]
  [11] Du B J, Song Y, Hu X S, et al. Int J Food Sci Technol, 2011, 46(5): 982
12. [12]
  [12] Wang H H, Dai J, Chen S W, et al. Food and Fermentation Industries (王浩豪, 戴军, 陈尚卫, 等. 食品与发酵工业), 2011, 37(12): 133
13. [13]
  [13] Gangola M P, Jaiswal S, Khedikar Y P, et al. Food Chem, 2014, 154: 127
14. [14]
  [14] Alpert A J. J Chromatogr, 1990, 499: 177
15. [15]
  [15] Hemstrom P, Irgum K. J Sep Sci, 2006, 29(12): 1784
16. [16]
  [16] Liang X M. Chinese Journal of Chromatography (梁鑫淼. 色谱), 2011, 29(3): 191
17. [17]
  [17] Ai L Z, Wu Y, Guo B H, et al. Shandong Food Fermentation (艾连中, 吴艳, 郭本恒, 等. 山东食品发酵), 2008(1): 39
18. [18]
  [18] He Y T, Pan X M. Food Science (何余堂, 潘孝明. 食品科学), 2010, 31(17): 493
1. [1]
  Wei Shao , Wanqun Zhang , Pingping Zhu , Wanqun Hu , Qiang Zhou , Weiwei Li , Kaiping Yang , Xisheng Wang . Design and Practice of Ideological and Political Cases in the Course of Instrument Analysis Experiment: Taking the GC-MS Experiment as an Example. University Chemistry, 2024, 39(2): 147-154. doi: 10.3866/PKU.DXHX202309048
2. [2]
  Xinyu ZENG , Guhua TANG , Jianming OUYANG . Inhibitory effect of Desmodium styracifolium polysaccharides with different content of carboxyl groups on the growth, aggregation and cell adhesion of calcium oxalate crystals. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1563-1576. doi: 10.11862/CJIC.20230374
3. [3]
  Wenjun Yang , Qiaoling Tan , Wenjiao Xie , Xiaoyu Pan , Youyong Yuan . Construction and Characterization of Calcium Alginate Microparticle Drug Delivery System: A Novel Design and Teaching Practice in Polymer Experiments. University Chemistry, 2025, 40(3): 371-380. doi: 10.12461/PKU.DXHX202405150
4. [4]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
5. [5]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
6. [6]
  Yukun Chang , Haoqin Huang , Baolei Wang . Preparation of Trans-Cinnamic Acid via “One-Pot” Protocol of Aldol Condensation-Hydrolysis Reaction: Recommending an Improved Organic Synthesis Experiment. University Chemistry, 2024, 39(4): 322-328. doi: 10.3866/PKU.DXHX202309095
7. [7]
  Qilu DU , Li ZHAO , Peng NIE , Bo XU . Synthesis and characterization of osmium-germyl complexes stabilized by triphenyl ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1088-1094. doi: 10.11862/CJIC.20240006
8. [8]
  Haiyu Nie , Chenhui Zhang , Fengpei Du . Ideological and Political Design for the Preparation, Characterization and Particle Size Control Experiment of Nanoemulsion. University Chemistry, 2024, 39(2): 41-46. doi: 10.3866/PKU.DXHX202306055
9. [9]
  Yongming Guo , Jie Li , Chaoyong Liu . Green Improvement and Educational Design in the Synthesis and Characterization of Silver Nanoparticles. University Chemistry, 2024, 39(3): 258-265. doi: 10.3866/PKU.DXHX202309057
10. [10]
  Yukai Jiang , Yihan Wang , Yunkai Zhang , Yunping Wei , Ying Ma , Na Du . Characterization and Phase Diagram of Surfactant Lyotropic Liquid Crystal. University Chemistry, 2024, 39(4): 114-118. doi: 10.3866/PKU.DXHX202309033
11. [11]
  Lei Shu , Zhengqing Hao , Kai Yan , Hong Wang , Lihua Zhu , Fang Chen , Nan Wang . Development of a Double-Carbon Related Experiment: Preparation, Characterization and Carbon-Capture Ability of Eggshell-Derived CaO. University Chemistry, 2024, 39(4): 149-156. doi: 10.3866/PKU.DXHX202310134
12. [12]
  Keweiyang Zhang , Zihan Fan , Liyuan Xiao , Haitao Long , Jing Jing . Unveiling Crystal Field Theory: Preparation, Characterization, and Performance Assessment of Nickel Macrocyclic Complexes. University Chemistry, 2024, 39(5): 163-171. doi: 10.3866/PKU.DXHX202310084
13. [13]
  Xinyuan Shi , Chenyangjiang , Changyu Zhai , Xuemei Lu , Jia Li , Zhu Mao . Preparation and Photoelectric Performance Characterization of Perovskite CsPbBr₃ Thin Films. University Chemistry, 2024, 39(6): 383-389. doi: 10.3866/PKU.DXHX202312019
14. [14]
  Yongjie ZHANG , Bintong HUANG , Yueming ZHAI . Research progress of formation mechanism and characterization techniques of protein corona on the surface of nanoparticles. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2318-2334. doi: 10.11862/CJIC.20240247
15. [15]
  Xueting Cao , Shuangshuang Cha , Ming Gong . 电催化反应中的界面双电层：理论、表征与应用. Acta Physico-Chimica Sinica, 2025, 41(5): 100041-. doi: 10.1016/j.actphy.2024.100041
16. [16]
  Hao Wu , Zhen Liu , Dachang Bai . ¹H NMR Spectrum of Amide Compounds. University Chemistry, 2024, 39(3): 231-238. doi: 10.3866/PKU.DXHX202309020
17. [17]
  Jinghua Wang , Yanxin Yu , Yanbiao Ren , Yesheng Wang . Integration of Science and Education: Investigation of Tributyl Citrate Synthesis under the Promotion of Hydrate Molten Salts for Research and Innovation Training. University Chemistry, 2024, 39(11): 232-240. doi: 10.3866/PKU.DXHX202402057
18. [18]
  Fei Liu , Dong-Yang Zhao , Kai Sun , Ting-Ting Yu , Xin Wang . Comprehensive Experimental Design for Photochemical Synthesis, Analysis, and Characterization of Seleno-Containing Medium-Sized N-Heterocycles. University Chemistry, 2024, 39(3): 369-375. doi: 10.3866/PKU.DXHX202309047
19. [19]
  Zunyuan Xie , Lijin Yang , Zixiao Wan , Xiaoyu Liu , Yushan He . Exploration of the Preparation and Characterization of Nano Barium Titanate and Its Application in Inorganic Chemistry Laboratory Teaching. University Chemistry, 2024, 39(4): 62-69. doi: 10.3866/PKU.DXHX202310137
20. [20]
  Yixuan Gao , Lingxing Zan , Wenlin Zhang , Qingbo Wei . Comprehensive Innovation Experiment: Preparation and Characterization of Carbon-based Perovskite Solar Cells. University Chemistry, 2024, 39(4): 178-183. doi: 10.3866/PKU.DXHX202311091

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(321)
HTML views(8)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142