Citation: GENG Lijuan, HUANG Junrong, FENG Feng, JIANG Pingping, CHU Xiaogang, ZHANG Feng, LING Yun. Determination of fructooligosaccharides in milk powder using high performance anion-exchange chromatography coupled with pulsed amperometric detection[J]. Chinese Journal of Chromatography, ;2014, 32(12): 1380-1384. doi: 10.3724/SP.J.1123.2014.07052 shu

Determination of fructooligosaccharides in milk powder using high performance anion-exchange chromatography coupled with pulsed amperometric detection

1.
1. College of Life Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China;

Corresponding author: HUANG Junrong, ZHANG Feng,
Received Date: 31 July 2014
Available Online: 25 August 2014
Fund Project: 质检总局公益性行业科研专项项目(2012104002) (2012104002)质检总局科技计划项目(2013IK198) (2013IK198)国家软科学研究计划项目(2010GXS5B153). (2010GXS5B153)

Fructooligosaccharides (FOS) are usually added in milk powder as a kind of prebiotic. Thus, quantitative analysis of the FOS is very important for the quality control of milk powder. In this study, a simple method for the simultaneous determination of three FOS components with degrees of polymerization (DP) 3-5 in milk powder was developed by high performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). The samples were extracted with 50%(v/v) ethanol aqueous solution and defatted by an On Guard RP pretreatment column. The separation was performed on a CarboPac PA200 column by gradient elution using deionized water, 0.2 mol/L NaOH solution and 0.4 mol/L NaAc solution as the mobile phases. The flow rate was 0.4 mL/min. The column temperature was 30 ℃; and the injection volume was 25 μL. Good linear response was observed in the concentration range of 0.05-10 mg/L (r²>0.9993). The limits of quantification were 0.02, 0.005 and 0.02 mg/L for 1-kestose, nystose and fructofuranosyl-nystose, respectively. The mean recoveries varied from 86.0% to 114.0% at three spiked levels of 0.5, 1.0 and 5.0 mg/L. The short-chain fructooligosaccharides from inulooligosaccharides (IOS) were successfully separated by the developed HPAEC-PAD method. The method is simple, accurate, sensitive, and helpful for the quality control of milk powder.
- high performance anion-exchange chromatography (HPAEC),
- pulsed amperometric detection (PAD),
- fructooligosaccharides (FOS),
- milk powder
1. [1]
  [1] GB/T 23528-2009
2. [2]
  [2] Ortiz L T, Rebolé A, Velasco S, et al. Aquacult Nutr, 2013, 19(4): 475
3. [3]
  [3] Nakada H, Sakae T, Watanabe T, et al. J Hard Tissue Biol, 2014, 23(1): 1
4. [4]
  [4] GB 14880-2012
5. [5]
  [5] Hu X Z, Wu J F. China Food Additives (胡学智, 伍剑锋. 中国食品添加剂), 2007(6): 148
6. [6]
  [6] Shin M S, Park J W, Cho M R, et al. Korean J Food Sci Technol, 2006, 38(6): 725
7. [7]
  [7] Ding H L, Li C, Jin P, et al. Chinese Journal of Chromatography (丁洪流, 李灿, 金萍, 等. 色谱), 2013, 31(8): 804
8. [8]
  [8] Downes K, Terry L A. Talanta, 2010, 82(1): 118
9. [9]
  [9] Li J, Liu X M, Zhou B, et al. J Agric Food Chem, 2013, 61(24): 5888
10. [10]
  [10] Borromei C, Cavazza A, Merusi C, et al. J Sep Sci, 2009, 32(21): 3635
11. [11]
  [11] Borromei C, Careri M, Cavazza A, et al. Int J Anal Chem, 2009, 2009: 1
12. [12]
  [12] Max F, Jinadevi S R, Audrey A. J Chromatogr B, 2009, 877(23): 2388
13. [13]
  [13] Seipert R R, Barboza M, Ninonuevo M R, et al. Anal Chem, 2008, 80(1): 159
14. [14]
  [14] Bai J, Jiang J D, Tao D L. Food Science (白靖, 姜金斗, 陶大利. 食品科学), 2014, 35(2): 257
15. [15]
  [15] Hoebregs H. J AOAC Int, 1997, 80(5): 1029
16. [16]
  [16] Zhang J M, Liu Y F, Tang H C, et al. Food Research and Development (张绩觅, 刘玉峰, 唐华澄, 等. 食品研究与开发), 2012, 33(1): 135
17. [17]
  [17] Corradini C, Cavazza A, Bignardi C. Int J Carbohydr Chem, 2012, 2012: 1
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