Citation: LI Zhongqin, LI Qiuyun, JIANG Xinglong, ZHANG Kun, GUAN Ruizhang. Isolation and preparation of gallic acid from Terminalia chebula Retz. with high-speed counter-current chromatography[J]. Chinese Journal of Chromatography, ;2014, 32(12): 1404-1408. doi: 10.3724/SP.J.1123.2014.07025 shu

Isolation and preparation of gallic acid from Terminalia chebula Retz. with high-speed counter-current chromatography

1.
Fisheries College, Jimei University, Engineering Research Centre of the Modern Industry Technology for Ministry of Education, Xiamen 361021, China

Corresponding author: GUAN Ruizhang,
Received Date: 16 July 2014
Available Online: 29 September 2014
Fund Project: 国家海洋局海洋经济创新发展区域示范专项(2012FJ03) (2012FJ03)厦门市重点项目(3502Z20110014,3502Z20123027) (3502Z20110014,3502Z20123027)鳗鲡现代产业技术教育部工程研究中心开放基金项目(RE201305) (RE201305)国家级大学生创新创业训练计划项目(Z81231). (Z81231)

A separation method based on high-speed counter-current chromatography (HSCCC) has been established for the isolation and preparation of gallic acid from the ethanol extract of Terminalia chebula Retz. After comparing five kinds of solvent protocols of HSCCC, the two-phase system of n-hexane-ethyl acetate-methanol-water (1:5:1:5, v/v/v/v) was finally chosen as the operating solvent of HSCCC for the separation of gallic acid, in which the lower phase was used as the mobile phase and the upper phase as stationary phase. The detection in the experiments was performed with an ultraviolet detector at 254 nm. Under the conditions of rotation speed of 850 r/min, lower phase flow rate of 2 mL/min, four peaks (Ⅰ/Ⅱ/Ⅲ/Ⅳ) were displayed on HSCCC chromatogram. Among them, only peak Ⅲ contained a large amount of gallic acid (about 96.40%), which was confirmed by electrospray ionization mass spectrometry (ESI-MS) and high performance liquid chromatographic (HPLC) analysis. As much as 8.6 mg of gallic acid was obtained from 100 mg of the ethanol extract of Terminalia chebula Retz., indicating the method is simple, robust and efficient for the preparation of gallic acid.
- high-speed counter-current chromatography (HSCCC),
- gallic acid,
- Terminalia chebula Retz
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沈阳化工大学材料科学与工程学院沈阳 110142