Citation: LI Zhongqin, LI Qiuyun, JIANG Xinglong, ZHANG Kun, GUAN Ruizhang. Isolation and preparation of gallic acid from Terminalia chebula Retz. with high-speed counter-current chromatography[J]. Chinese Journal of Chromatography, ;2014, 32(12): 1404-1408. doi: 10.3724/SP.J.1123.2014.07025 shu

Isolation and preparation of gallic acid from Terminalia chebula Retz. with high-speed counter-current chromatography

1.
Fisheries College, Jimei University, Engineering Research Centre of the Modern Industry Technology for Ministry of Education, Xiamen 361021, China

Corresponding author: GUAN Ruizhang,
Received Date: 16 July 2014
Available Online: 29 September 2014
Fund Project: 国家海洋局海洋经济创新发展区域示范专项(2012FJ03) (2012FJ03)厦门市重点项目(3502Z20110014,3502Z20123027) (3502Z20110014,3502Z20123027)鳗鲡现代产业技术教育部工程研究中心开放基金项目(RE201305) (RE201305)国家级大学生创新创业训练计划项目(Z81231). (Z81231)

A separation method based on high-speed counter-current chromatography (HSCCC) has been established for the isolation and preparation of gallic acid from the ethanol extract of Terminalia chebula Retz. After comparing five kinds of solvent protocols of HSCCC, the two-phase system of n-hexane-ethyl acetate-methanol-water (1:5:1:5, v/v/v/v) was finally chosen as the operating solvent of HSCCC for the separation of gallic acid, in which the lower phase was used as the mobile phase and the upper phase as stationary phase. The detection in the experiments was performed with an ultraviolet detector at 254 nm. Under the conditions of rotation speed of 850 r/min, lower phase flow rate of 2 mL/min, four peaks (Ⅰ/Ⅱ/Ⅲ/Ⅳ) were displayed on HSCCC chromatogram. Among them, only peak Ⅲ contained a large amount of gallic acid (about 96.40%), which was confirmed by electrospray ionization mass spectrometry (ESI-MS) and high performance liquid chromatographic (HPLC) analysis. As much as 8.6 mg of gallic acid was obtained from 100 mg of the ethanol extract of Terminalia chebula Retz., indicating the method is simple, robust and efficient for the preparation of gallic acid.
- high-speed counter-current chromatography (HSCCC),
- gallic acid,
- Terminalia chebula Retz
1. [1]
  [1] Guo J, Yang H L. Journal of Pathogen Biology (郭军, 杨恒林. 中国病原生物学杂志), 2013, 8(1): 89
2. [2]
  [2] Zhang R. Animal Husbandry and Feed Science (张蕊. 畜牧与饲料科学), 2013, 34(5): 13
3. [3]
  [3] Pan G Q, Lu Y C, D J C M. Chinese Traditional and Herbal Drugs (潘国庆, 卢永昌, 德吉措姆. 中草药), 2006, 37(9): 1350
4. [4]
  [4] Ke F M, Zhang K L. Journal of Luzhou Medical College (柯发敏, 张开莲. 泸州医学院学报), 2011, 34(4): 440
5. [5]
  [5] Lu J, Wang Z N, Chen R, et al. Acta Hydrobiologica Sinica (卢静, 王振宁, 陈锐, 等. 水生生物学报), 2013, 37(6): 1128
6. [6]
  [6] Ito Y. J Chromatogr A, 2005, 1065(2): 145
7. [7]
  [7] Chen S W, Pan Y Q, Cai J Q. Tianjin Pharmacy (陈苏伟, 潘勇琴, 蔡纪青. 天津药学), 2008, 20(1): 74
8. [8]
  [8] Li X D, Li X G, Song S H, et al. Chinese Journal of Chromatography (李小多, 李学刚, 宋尚华, 等. 色谱), 2012, 30(9): 971
9. [9]
  [9] Han Q B, Song J Z, Qiao C F, et al. J Sep Sci, 2006, 29(11): 1653
10. [10]
  [10] Yao S, Li Y, Kong L Y. J Chromatogr A, 2006, 1115(1/2): 64
11. [11]
  [11] Liu Y L, Chen T, Chen C, et al. Chinese Journal of Chromatography (刘永玲, 陈涛, 陈晨, 等. 色谱), 2014, 32(5): 543
12. [12]
  [12] Tian G L, Zhang T Y, Yang F Q. J Chromatogr A, 2000, 886(1/2): 309
13. [13]
  [13] Chen X X, Zhang L Y, Wan J Z, et al. China Journal of Chinese Materia Medica (陈欣霞, 张丽艳, 万金志, 等. 中国中药杂志), 2010, 35(15): 1957
14. [14]
  [14] Lu J J, Wei Y, Yuan Q P. Sep Purif Technol, 2007, 55(1): 40
1. [1]
  Jingming Li , Bowen Ding , Nan Li , Nurgul . Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching. University Chemistry, 2024, 39(8): 263-269. doi: 10.3866/PKU.DXHX202312078
2. [2]
  Huanhuan XIE , Yingnan SONG , Lei LI . Two-dimensional single-layer BiOI nanosheets: Lattice thermal conductivity and phonon transport mechanism. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 702-708. doi: 10.11862/CJIC.20240281
3. [3]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
4. [4]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
5. [5]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
6. [6]
  Yong Shu , Xing Chen , Sai Duan , Rongzhen Liao . How to Determine the Equilibrium Bond Distance of Homonuclear Diatomic Molecules: A Case Study of H₂. University Chemistry, 2024, 39(7): 386-393. doi: 10.3866/PKU.DXHX202310102
7. [7]
  Qingying Gao , Tao Luo , Jianyuan Su , Chaofan Yu , Jiazhu Li , Bingfei Yan , Wenzuo Li , Zhen Zhang , Yi Liu . Refinement and Expansion of the Classic Cinnamic Acid Synthesis Experiment. University Chemistry, 2024, 39(5): 243-250. doi: 10.3866/PKU.DXHX202311074
8. [8]
  Yufang GAO , Nan HOU , Yaning LIANG , Ning LI , Yanting ZHANG , Zelong LI , Xiaofeng LI . Nano-thin layer MCM-22 zeolite: Synthesis and catalytic properties of trimethylbenzene isomerization reaction. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1079-1087. doi: 10.11862/CJIC.20240036
9. [9]
  Meijin Li , Xirong Fu , Xue Zheng , Yuhan Liu , Bao Li . The Marvel of NAD⁺: Nicotinamide Adenine Dinucleotide. University Chemistry, 2024, 39(9): 35-39. doi: 10.12461/PKU.DXHX202401027
10. [10]
  Keying Qu , Jie Li , Ziqiu Lai , Kai Chen . Unveiling the Mystery of Chirality from Tartaric Acid. University Chemistry, 2024, 39(9): 369-378. doi: 10.12461/PKU.DXHX202310091
11. [11]
  Ran Yu , Chen Hu , Ruili Guo , Ruonan Liu , Lixing Xia , Cenyu Yang , Jianglan Shui . 杂多酸H₃PW₁₂O₄₀高效催化MgH₂储氢. Acta Physico-Chimica Sinica, 2025, 41(1): 2308032-. doi: 10.3866/PKU.WHXB202308032
12. [12]
  Zijian Zhao , Yanxin Shi , Shicheng Li , Wenhong Ruan , Fang Zhu , Jijun Jiang . A New Exploration of the Preparation of Polyacrylic Acid by Free Radical Polymerization Based on the Concept of Green Chemistry. University Chemistry, 2024, 39(5): 315-324. doi: 10.3866/PKU.DXHX202311094
13. [13]
  Ping Song , Nan Zhang , Jie Wang , Rui Yan , Zhiqiang Wang , Yingxue Jin . Experimental Teaching Design on Synthesis and Antitumor Activity Study of Cu-Pyropheophorbide-a Methyl Ester. University Chemistry, 2024, 39(6): 278-286. doi: 10.3866/PKU.DXHX202310087
14. [14]
  Jiahe LIU , Gan TANG , Kai CHEN , Mingda ZHANG . Effect of low-temperature electrolyte additives on low-temperature performance of lithium cobaltate batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 719-728. doi: 10.11862/CJIC.20250023
15. [15]
  Junke LIU , Kungui ZHENG , Wenjing SUN , Gaoyang BAI , Guodong BAI , Zuwei YIN , Yao ZHOU , Juntao LI . Preparation of modified high-nickel layered cathode with LiAlO₂/cyclopolyacrylonitrile dual-functional coating. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1461-1473. doi: 10.11862/CJIC.20240189
16. [16]
  Jizhou Liu , Chenbin Ai , Chenrui Hu , Bei Cheng , Jianjun Zhang . 六氯锡酸铵促进钙钛矿太阳能电池界面电子转移及其飞秒瞬态吸收光谱研究. Acta Physico-Chimica Sinica, 2024, 40(11): 2402006-. doi: 10.3866/PKU.WHXB202402006
17. [17]
  Ke Li , Chuang Liu , Jingping Li , Guohong Wang , Kai Wang . 钛酸铋/氮化碳无机有机复合S型异质结纯水光催化产过氧化氢. Acta Physico-Chimica Sinica, 2024, 40(11): 2403009-. doi: 10.3866/PKU.WHXB202403009
18. [18]
  Yukun Chang , Haoqin Huang , Baolei Wang . Preparation of Trans-Cinnamic Acid via “One-Pot” Protocol of Aldol Condensation-Hydrolysis Reaction: Recommending an Improved Organic Synthesis Experiment. University Chemistry, 2024, 39(4): 322-328. doi: 10.3866/PKU.DXHX202309095
19. [19]
  Yang Chen , Peng Chen , Yuyang Song , Yuxue Jin , Song Wu . Application of Chemical Transformation Driven Impurity Separation in Experiments Teaching: A Novel Method for Purification of α-Fluorinated Mandelic Acid. University Chemistry, 2024, 39(6): 253-263. doi: 10.3866/PKU.DXHX202310077
20. [20]
  Lirui Shen , Kun Liu , Ying Yang , Dongwan Li , Wengui Chang . Synthesis and Application of Decanedioic Acid-N-Hydroxysuccinimide Ester: Exploration of Teaching Reform in Comprehensive Applied Chemistry Experiment. University Chemistry, 2024, 39(8): 212-220. doi: 10.3866/PKU.DXHX202312035

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(218)
HTML views(16)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142