Citation: SHAN Guangzhi, ZONG Yanping, WANG Xiao, LU Jinghua. Determination of principal components and related substances in adenosine disodium triphosphate preparation by ion chromatography[J]. Chinese Journal of Chromatography, ;2014, 32(11): 1275-1279. doi: 10.3724/SP.J.1123.2014.07012 shu

Determination of principal components and related substances in adenosine disodium triphosphate preparation by ion chromatography

1.
1. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing 100050, China;

Corresponding author: SHAN Guangzhi,
Received Date: 4 July 2014
Available Online: 30 July 2014
Fund Project: 科技部新药创制重大专项平台项目(2012ZX09301002-001-019). (2012ZX09301002-001-019)

A new method was developed for the determination of adenosine disodium triphosphate (ATP-Na₂) and its related substances in ATP-Na₂ preparation by ion chromatography (IC). The sample was diluted with ultrapure water and filtrated by 0.22 μm polyether sulfone filter membrance, and then analyzed by IC directly without any more pretreatment. The analysis was performed on a Dionex IonPac AS11-HC column(250 mm×4 mm) and a guard column IonPac AG11-HC (50 mm×4 mm). A KOH eluent generator cartridge was used for gradient elution at the flow rate of 1.0 mL/min. The detection was performed by a Dionex suppressed (Dionex AERS 500 4-mm) conductivity detector. The injection volume was 10 μL. The assay was quantitatively completed by external standard method and the related substances were calculated with correction factors. The linear ranges of the method for ATP-Na₂, adenosine disodium diphosphate (ADP-Na₂) and adenosine disodium monophosphate (AMP-Na₂) were 0.000146-1.83 g/L (r=0.9997), 0.000484-1.51 g/L (r=0.9996) and 0.000426-0.804 g/L (r=0.9999), respectively. The average recoveries of ATP-Na₂ were 96.50%, 96.57% and 96.77% at three spiked levels. The limits of quantitation (S/N=10) of ATP-Na₂, ADP-Na₂ and AMP-Na₂ were 1.5 ng, 4.8 ng, 4.3 ng, and the limits of detection (S/N=3) were 0.58 ng, 1.21 ng, 1.28 ng, respectively. The results demonstrated that the system has the advantages of high sensitivity, facile automation and simple sample pretreatment. The method is suitable for the quality control of adenosine disodium triphosphate preparations.
- ion chromatography (IC),
- conductivity detector,
- adenosine disodium triphosphate (ATP-Na₂),
- related substances,
- preparation
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