Citation: SHAN Guangzhi, ZONG Yanping, WANG Xiao, LU Jinghua. Determination of principal components and related substances in adenosine disodium triphosphate preparation by ion chromatography[J]. Chinese Journal of Chromatography, ;2014, 32(11): 1275-1279. doi: 10.3724/SP.J.1123.2014.07012 shu

Determination of principal components and related substances in adenosine disodium triphosphate preparation by ion chromatography

1.
1. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing 100050, China;

Corresponding author: SHAN Guangzhi,
Received Date: 4 July 2014
Available Online: 30 July 2014
Fund Project: 科技部新药创制重大专项平台项目(2012ZX09301002-001-019). (2012ZX09301002-001-019)

A new method was developed for the determination of adenosine disodium triphosphate (ATP-Na₂) and its related substances in ATP-Na₂ preparation by ion chromatography (IC). The sample was diluted with ultrapure water and filtrated by 0.22 μm polyether sulfone filter membrance, and then analyzed by IC directly without any more pretreatment. The analysis was performed on a Dionex IonPac AS11-HC column(250 mm×4 mm) and a guard column IonPac AG11-HC (50 mm×4 mm). A KOH eluent generator cartridge was used for gradient elution at the flow rate of 1.0 mL/min. The detection was performed by a Dionex suppressed (Dionex AERS 500 4-mm) conductivity detector. The injection volume was 10 μL. The assay was quantitatively completed by external standard method and the related substances were calculated with correction factors. The linear ranges of the method for ATP-Na₂, adenosine disodium diphosphate (ADP-Na₂) and adenosine disodium monophosphate (AMP-Na₂) were 0.000146-1.83 g/L (r=0.9997), 0.000484-1.51 g/L (r=0.9996) and 0.000426-0.804 g/L (r=0.9999), respectively. The average recoveries of ATP-Na₂ were 96.50%, 96.57% and 96.77% at three spiked levels. The limits of quantitation (S/N=10) of ATP-Na₂, ADP-Na₂ and AMP-Na₂ were 1.5 ng, 4.8 ng, 4.3 ng, and the limits of detection (S/N=3) were 0.58 ng, 1.21 ng, 1.28 ng, respectively. The results demonstrated that the system has the advantages of high sensitivity, facile automation and simple sample pretreatment. The method is suitable for the quality control of adenosine disodium triphosphate preparations.
- ion chromatography (IC),
- conductivity detector,
- adenosine disodium triphosphate (ATP-Na₂),
- related substances,
- preparation
1. [1]
  [1] Jager R, Roberts M D, Lowery R P, et al. J Int Soc Sport Nutr, 2014, 11(1): 28
2. [2]
  [2] Li Y F, Dong N, Cheng X D, et al. Journal of Clinical Emergency Call (李运峰, 董宁, 程向东, 等. 临床急诊杂志), 2004, 5(3): 4
3. [3]
  [3] Yi S Y, Zhi D S. Diagnosis and Treatment of Traumatic Brain Injury. Beijing: People's Medical Publishing House (易生禹, 只达石. 颅脑损伤诊治. 北京: 人民卫生出版社), 2000: 110
4. [4]
  [4] Pharmacopoeia Commission of People's Republic of China. Pharmacopoeia of People's Republic of China, Part 2. Beijing: China Medical Science and Technology Press (国家药典委员会. 中华人民共和国药典, 二部. 北京: 中国医药科技出版社), 2010: 28
5. [5]
  [5] Pharmacopoeia Commission of People's Republic of China. The First Addendum of Pharmacopoeia of People's Republic of China. Beijing: China Medical Science and Technology Press (国家药典委员会. 中华人民共和国药典第一增补本. 北京: 中国医药科技出版社), 2010: 313
6. [6]
  [6] The Quality Standards for Drug of China, National Drug Standard Provincial Standard of Chemical Drug Up to National Standard. Vol. 9 (国家药品标准, 化学药品地方标准上升国家标准. 第9册). 2002: 9.[2014-07-04]. http://db.yaozh.com/content.php?action=biaozhun&uid=41360
7. [7]
  [7] State Food and Drug Administration Standards (国家食品药品监督管理局标准). YBH22872005.[2014-07-04]. http:// db.yaozh.com/biaozhun/huayaobz1-16/ws-10001-(HD-0787)-2002.htm
8. [8]
  [8] Mo Z L. Strait Pharmaceutical Journal (莫姿丽. 海峡药学), 2011, 23(9): 75
9. [9]
  [9] Drug Standard of Ministry of Public Health[Two Sixth Volumes (Biochemical Medicine Article)] (卫生部药品标准[二部第六册(生化药品第一分册)]), 1998.[2014-07-04]. http://db.yaozh.com/content.php?action=biaozhun&uid=18243
10. [10]
  [10] Wang X Y, Lin Y. Chinese Journal of Biochemical Pharmaceutics (王学艳, 林英. 中国生化药物杂志), 2001, 22(3): 151
11. [11]
  [11] Hao S L, Xu K S. Chinese Journal of Biochemical Pharmaceutics (郝苏丽, 徐康森. 中国生化药物杂志), 2001, 22(6): 305
12. [12]
  [12] Chen Q H, Li P, Zhu J. China Pharmaceuticals (陈琴华, 李鹏, 朱军. 中国药业), 2013, 22(18): 22
13. [13]
  [13] Zhao L, Sheng B Y. Chinese Journal of Modern Applied Pharmacy (赵岚, 盛炳义. 中国现代应用药学), 2002, 19(5): 403
14. [14]
  [14] Chen Y, Liu Y X, Chen Z D, et al. Chinese Journal of Chromatography (陈郁, 刘玉秀, 陈智栋, 等. 色谱), 2012, 30(4): 414
15. [15]
  [15] Hu Z Y, Ye M L, Pan G W, et al. Chinese Journal of Chromatography (胡忠阳, 叶明立, 潘广文, 等. 色谱), 2012, 30(4): 391
16. [16]
  [16] Zhang T T, Wang N N, Ye M L, et al. Chinese Journal of Chromatography (张婷婷, 王娜妮, 叶明立, 等. 色谱), 2013, 31(1): 88
17. [17]
  [17] Lin L, Wang H B, Shi Y L. Chinese Journal of Chromatography (林立, 王海波, 史亚利. 色谱), 2013, 31(3): 281
18. [18]
  [18] Wang Y, Geng Q, Zuo Y X, et al. Chinese Journal of Chromatography (王勇, 耿庆, 左跃先, 等. 色谱), 2013, 31(9): 920
19. [19]
  [19] Mou S F, Liu K N, Ding X J. Ion Chromatographic Method and Its Application. 2nd ed. Beijing: Chemical Industry Press (牟世芬, 刘克纳, 丁晓静. 离子色谱方法及应用. 2版. 北京: 化学工业出版社), 2005: 135
1. [1]
  Liuyun Chen , Wenju Wang , Tairong Lu , Xuan Luo , Xinling Xie , Kelin Huang , Shanli Qin , Tongming Su , Zuzeng Qin , Hongbing Ji . 软模板法诱导Cu/Al₂O₃深孔道结构促进等离子催化CO₂加氢制二甲醚. Acta Physico-Chimica Sinica, 2025, 41(6): 100054-. doi: 10.1016/j.actphy.2025.100054
2. [2]
  Peiran ZHAO , Yuqian LIU , Cheng HE , Chunying DUAN . A functionalized Eu³⁺ metal-organic framework for selective fluorescent detection of pyrene. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 713-724. doi: 10.11862/CJIC.20230355
3. [3]
  Yongzhi LI , Han ZHANG , Gangding WANG , Yanwei SUI , Lei HOU , Yaoyu WANG . A two-dimensional metal-organic framework for the determination of nitrofurantoin and nitrofurazone in aqueous solution. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 245-253. doi: 10.11862/CJIC.20240307
4. [4]
  Shengbiao Zheng , Liang Li , Nini Zhang , Ruimin Bao , Ruizhang Hu , Jing Tang . Metal-Organic Framework-Derived Materials Modified Electrode for Electrochemical Sensing of Tert-Butylhydroquinone: A Recommended Comprehensive Chemistry Experiment for Translating Research Results. University Chemistry, 2024, 39(7): 345-353. doi: 10.3866/PKU.DXHX202310096
5. [5]
  Yinuo Wang , Siran Wang , Yilong Zhao , Dazhen Xu . Selective Synthesis of Diarylmethyl Anilines and Triarylmethanes via Multicomponent Reactions: Introduce a Comprehensive Experiment of Organic Chemistry. University Chemistry, 2024, 39(8): 324-330. doi: 10.3866/PKU.DXHX202401063
6. [6]
  Zhuo WANG , Xiaotong LI , Zhipeng HU , Junqiao PAN . Three-dimensional porous carbon decorated with nano bismuth particles: Preparation and sodium storage properties. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 267-274. doi: 10.11862/CJIC.20240223
7. [7]
  Xiuyun Wang , Jiashuo Cheng , Yiming Wang , Haoyu Wu , Yan Su , Yuzhuo Gao , Xiaoyu Liu , Mingyu Zhao , Chunyan Wang , Miao Cui , Wenfeng Jiang . Improvement of Sodium Ferric Ethylenediaminetetraacetate (NaFeEDTA) Iron Supplement Preparation Experiment. University Chemistry, 2024, 39(2): 340-346. doi: 10.3866/PKU.DXHX202308067
8. [8]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
9. [9]
  Mengyao Shi , Kangle Su , Qingming Lu , Bin Zhang , Xiaowen Xu . Determination of Potassium Content in Tobacco Stem Ash by Flame Atomic Absorption Spectroscopy. University Chemistry, 2024, 39(10): 255-260. doi: 10.12461/PKU.DXHX202404105
10. [10]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
11. [11]
  Zhuo Wang , Xue Bai , Kexin Zhang , Hongzhi Wang , Jiabao Dong , Yuan Gao , Bin Zhao . MOF模板法合成氮掺杂碳材料用于增强电化学钠离子储存和去除. Acta Physico-Chimica Sinica, 2025, 41(3): 2405002-. doi: 10.3866/PKU.WHXB202405002
12. [12]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
13. [13]
  Renxiao Liang , Zhe Zhong , Zhangling Jin , Lijuan Shi , Yixia Jia . A Palladium/Chiral Phosphoric Acid Relay Catalysis for the One-Pot Three-Step Synthesis of Chiral Tetrahydroquinoline. University Chemistry, 2024, 39(5): 209-217. doi: 10.3866/PKU.DXHX202311024
14. [14]
  Li'na ZHONG , Jingling CHEN , Qinghua ZHAO . Synthesis of multi-responsive carbon quantum dots from green carbon sources for detection of iron ions and L-ascorbic acid. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 709-718. doi: 10.11862/CJIC.20240280
15. [15]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
16. [16]
  Jiaojiao Yu , Bo Sun , Na Li , Cong Wen , Wei Li . Improvement of Classical Organic Experiment Based on the “Reverse-Step Optimization Method”: Taking Synthesis of Ethyl Acetate as an Example. University Chemistry, 2025, 40(3): 333-341. doi: 10.12461/PKU.DXHX202405177
17. [17]
  Xiaoning TANG , Shu XIA , Jie LEI , Xingfu YANG , Qiuyang LUO , Junnan LIU , An XUE . Fluorine-doped MnO₂ with oxygen vacancy for stabilizing Zn-ion batteries. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1671-1678. doi: 10.11862/CJIC.20240149
18. [18]
  Wendian XIE , Yuehua LONG , Jianyang XIE , Liqun XING , Shixiong SHE , Yan YANG , Zhihao HUANG . Preparation and ion separation performance of oligoether chains enriched covalent organic framework membrane. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1528-1536. doi: 10.11862/CJIC.20240050
19. [19]
  Ran HUO , Zhaohui ZHANG , Xi SU , Long CHEN . Research progress on multivariate two dimensional conjugated metal organic frameworks. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2063-2074. doi: 10.11862/CJIC.20240195
20. [20]
  Jianbao Mei , Bei Li , Shu Zhang , Dongdong Xiao , Pu Hu , Geng Zhang . Enhanced Performance of Ternary NASICON-Type Na_3.5-xMn_0.5V_1.5-xZr_x(PO₄)₃/C Cathodes for Sodium-Ion Batteries. Acta Physico-Chimica Sinica, 2024, 40(12): 2407023-. doi: 10.3866/PKU.WHXB202407023

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(301)
HTML views(25)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142