Citation: GUO Shoudong, SANG Hui, YANG Nana, KAN Yujie, LI Fuyu, LI Yu, LI Fangyuan, QIN Shucun. Measurement of sialic acid from lipoproteins and human plasma by liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(11): 1197-1200. doi: 10.3724/SP.J.1123.2014.07002 shu

Measurement of sialic acid from lipoproteins and human plasma by liquid chromatography-tandem mass spectrometry

1.
Key Laboratory of Atherosclerosis in Universities of Shandong, Taishan Medical University, Taian 271000, China

Corresponding author: QIN Shucun,
Received Date: 2 July 2014
Available Online: 14 August 2014
Fund Project: 国家自然科学基金(31300639,81170785) (31300639,81170785)山东省青年基金项目(ZR2013HQ014) (ZR2013HQ014)山东省中医药科学技术研究项目(2013-257) (2013-257)泰山医学院博士启动基金项目(2234). (2234)

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established to quantify sialic acid (N-acetylneuraminic acid, NANA) from lipoproteins and human plasma. The method was used to investigate the different contents of NANA from lipoproteins between diabetic with an average age of 51.6 years and healthy participants with an average age of 50.7 years. The NANA from lipoprotein samples was hydrolyzed by acetic acid (pH=2) at 80 ℃ for 2 h and analyzed by the optimized LC-MS/MS method after high speed centrifugation and filtration. The limits of detection and quantification of NANA were 7.4 and 24.5 pg, respectively. The linear range was 2.5-80 ng/mL for NANA and the correlation coefficient (R²) was more than 0.998. The levels of NANA in the plasma of type Ⅱ diabetics and healthy participants were (548.3±88.9) and (415.3±55.5) mg/L, respectively; and the levels of NANA from very low density lipoproteins (VLDL), low density lipoproteins (LDL), and high density lipoproteins (HDL) of the type Ⅱ diabetics and the healthy participants were (4.91±0.19), (6.95±0.28), (3.61±0.22) μg/mg and (2.90±0.27), (7.03±0.04), (2.40±0.09) μg/mg, respectively. The sialic acid levels of VLDL and HDL from the type Ⅱ diabetics were markedly higher than those of the corresponding healthy participants with the similar ages (P<0.01). The method can rapidly determine the sialic acid from lipoproteins, and is reproducible and time saving.
- liquid chromatography-tandem mass spectrometry (LC-MS/MS),
- sialic acid,
- lipoprotein,
- human plasma,
- diabetes mellitus
1. [1]
  [1] Dodani S, Grice D G, Joshi S. J Clin Lipidol, 2009, 3(2): 70
2. [2]
  [2] Millar J S, Anber V, Shepherd J, et al. Atherosclerosis, 1999, 145: 253
3. [3]
  [3] Cong P X, Li Z J, Xu J, et al. Chinese Journal of Chromatography (丛培旭, 李兆杰, 徐杰, 等. 色谱), 2013, 31(5): 399
4. [4]
  [4] Camejo G, López A, López F, et al. Atherosclerosis, 1985, 55: 93
5. [5]
  [5] Stratton P D, Lumb P J, Paganga G, et al. Atherosclerosis, 2001, 154: 285
6. [6]
  [6] Gavella M, Lipovac V, Car A, et al. Acta Diabetol, 2003, 40: 95
7. [7]
  [7] Serdar Z, Yesilbursa D, Dirican M, et al. Cell Biochem Funct, 2007, 25: 655
8. [8]
  [8] Anumula K R. Anal Biochem, 1995, 230: 24
9. [9]
  [9] Hikita T, Tadano-Aritomi K, Iida-Tanaka N, et al. Anal Biochem, 2000, 281: 193
10. [10]
  [10] Reuter G, Pfeil R, Stoll S, et al. Eur J Biochem, 1983, 134: 139
11. [11]
  [11] Ortner K, Buchberger W. Electrophoresis, 2008, 29(10): 2233
12. [12]
  [12] Rajendiran S, Lakshamanappa H S, Zachariah B, et al. Am J Trop Med Hyg, 2008, 79(3): 372
13. [13]
  [13] Kontush A, John Chapman M. Pharmacol Rev, 2006, 58: 342
14. [14]
  [14] Phillips C, Owens D, Collins P, et al. Atherosclerosis, 2005, 181: 109
15. [15]
  [15] Iijima R, Takahashi H, Namme R, et al. FEBS Lett, 2004, 561: 163
16. [16]
  [16] Ogasawara Y, Namai T, Yoshino F, et al. FEBS Lett, 2007, 581(13): 2473
17. [17]
  [17] Lindbohm N, Gylling H, Miettinen T A. J Lipid Res, 2000, 41: 1110
18. [18]
  [18] Filipovic I, Schwarzmann G, Mraz W, et al. Eur J Biochem, 1979, 93: 51
19. [19]
  [19] Xie H L, Li C, Liu N. Chinese Journal of Chromatography (解鸿蕾, 李春, 刘宁. 色谱), 2013, 31(8): 781
20. [20]
  [20] Hou X C, Zhu L P, Liu C S, et al. Modern Food Science and Technology (侯向昶, 朱丽萍, 刘春生, 等. 现代食品科技), 2013, 29(7): 1706
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