Citation: GAO Mengjie, ZHOU Yao, SHENG Yonggang, ZHAO Shanzhen, DENG Xiaojun, GUO Dehua, DING Zhuoping, WANG Guomin, PENG Tao. Determination of 12 bisphenol substances in functional foods by QuEChERS and high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(11): 1201-1208. doi: 10.3724/SP.J.1123.2014.06037 shu

Determination of 12 bisphenol substances in functional foods by QuEChERS and high performance liquid chromatography-tandem mass spectrometry

1.
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;

Corresponding author: DENG Xiaojun, DING Zhuoping,
Received Date: 25 June 2014
Available Online: 19 August 2014
Fund Project: 上海市科委技术标准专项项目(13DZ0502502,12DZ0503102) (13DZ0502502,12DZ0503102)科技部"十二五"科技支撑项目(2012BAD33B02) (2012BAD33B02)国家质检总局质检公益专项(201310140-02). (201310140-02)

A method based on high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for the simultaneous determination of 12 bisphenol substances in functional foods (powder, tablet, capsule) was presented. The samples were extracted by acetonitrile containing 1% (v/v) acetic acid followed by further cleaned up using matrix solid-phase dispersion to remove matrix interferences. The separation of the 12 bisphenol substances was performed on a Thermo Aquasil C₁₈ column (150 mm×4.6 mm, 3.0 μm), and determined in the positive and negative MRM modes by MS/MS using matrix-matched external standard method. The results demonstrated that the calibration curves were of good linearity with the correlation coefficients greater than 0.99. The limits of detection (LODs, S/N>3) were in the range of 0.1-0.5 μg/kg and the limits of quantitation (LOQs, S/N>10) were 0.4-1.7 μg/kg. The recoveries of the 12 bisphenol substances spiked at three levels (2.0, 5.0 and 10.0 μg/kg) in matrix ranged from 60.5% to 116.3% with the relative standard deviations (RSDs) of 6.8% to 11.2%. The established method is simple, time-saving and sensitive. It can meet the requirements for current regulations while achieving qualitative and quantitative determination of the 12 bisphenol substances in functional foods.
- QuEChERS,
- high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),
- bisphenol substances,
- functional food
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