Citation: GAO Mengjie, ZHOU Yao, SHENG Yonggang, ZHAO Shanzhen, DENG Xiaojun, GUO Dehua, DING Zhuoping, WANG Guomin, PENG Tao. Determination of 12 bisphenol substances in functional foods by QuEChERS and high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(11): 1201-1208. doi: 10.3724/SP.J.1123.2014.06037 shu

Determination of 12 bisphenol substances in functional foods by QuEChERS and high performance liquid chromatography-tandem mass spectrometry

1.
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;

Corresponding author: DENG Xiaojun, DING Zhuoping,
Received Date: 25 June 2014
Available Online: 19 August 2014
Fund Project: 上海市科委技术标准专项项目(13DZ0502502,12DZ0503102) (13DZ0502502,12DZ0503102)科技部"十二五"科技支撑项目(2012BAD33B02) (2012BAD33B02)国家质检总局质检公益专项(201310140-02). (201310140-02)

A method based on high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for the simultaneous determination of 12 bisphenol substances in functional foods (powder, tablet, capsule) was presented. The samples were extracted by acetonitrile containing 1% (v/v) acetic acid followed by further cleaned up using matrix solid-phase dispersion to remove matrix interferences. The separation of the 12 bisphenol substances was performed on a Thermo Aquasil C₁₈ column (150 mm×4.6 mm, 3.0 μm), and determined in the positive and negative MRM modes by MS/MS using matrix-matched external standard method. The results demonstrated that the calibration curves were of good linearity with the correlation coefficients greater than 0.99. The limits of detection (LODs, S/N>3) were in the range of 0.1-0.5 μg/kg and the limits of quantitation (LOQs, S/N>10) were 0.4-1.7 μg/kg. The recoveries of the 12 bisphenol substances spiked at three levels (2.0, 5.0 and 10.0 μg/kg) in matrix ranged from 60.5% to 116.3% with the relative standard deviations (RSDs) of 6.8% to 11.2%. The established method is simple, time-saving and sensitive. It can meet the requirements for current regulations while achieving qualitative and quantitative determination of the 12 bisphenol substances in functional foods.
- QuEChERS,
- high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),
- bisphenol substances,
- functional food
1. [1]
  [1] Cabado A G, Aldea S, Porro C, et al. Food Chem Toxicol, 2008, 46: 1674
2. [2]
  [2] Miao J Z, Xue M, Zhang H. Chinese Journal of Analytical Chemistry (缪佳铮, 薛鸣, 张虹. 分析化学), 2009, 37(6): 911
3. [3]
  [3] Cao G Z, Chen S H, Xiao D Q, et al. Chinese Journal of Analytical Chemistry (曹国洲, 陈少鸿, 肖道清, 等. 分析化学), 2014, 42(3): 403
4. [4]
  [4] Bao Y, Wang H Y, Li Z Q, et al. Food Science (鲍洋, 汪何雅, 李竹青, 等. 食品科学), 2011, 32(21): 261
5. [5]
  [5] Hu X W, Zhang W D, Liu Y Q. Food Science (胡向蔚, 张文德, 刘炎桥. 食品科学), 2006, 27(4): 264
6. [6]
  [6] Cao G P, Wang B B, Ding Q C, et al. Chemistry & Bioengineering (曹桂萍, 王蓓蓓, 丁其晨, 等. 化学与生物工程), 2010, 27(10): 86
7. [7]
  [7] Commission Regulation EC/1895/2005
8. [8]
  [8] Commission Regulation EU No.10/2011
9. [9]
  [9] GB 9685-2008
10. [10]
  [10] Zhao X Y, Fu X F, Wang P, et al. Chinese Journal of Chromatography (赵晓亚, 付小芳, 王鹏, 等. 色谱), 2012, 30(10): 1002
11. [11]
  [11] Liang K, Deng X J, Yi X H, et al. Chinese Journal of Analytical Chemistry (梁凯, 邓晓军, 伊雄海, 等. 分析化学), 2012, 40(5): 705
12. [12]
  [12] Zhang Z H, Luo S L, Wu S L, et al. Journal of Instrumental Analysis (张朝晖, 罗生亮, 吴少林, 等. 分析测试学报), 2009, 28(6): 714
13. [13]
  [13] Xiao D Q, Liu Z M, Ma M, et al. Journal of Instrumental Analysis (肖道清, 刘在美, 马明, 等. 分析测试学报), 2013, 32(12): 1502
14. [14]
  [14] Lehotay S J, Mastovska K, Yun S J. J AOAC Int, 2005, 88(2): 630
15. [15]
  [15] Xu R, Wu J W, Liu Y G, et al. Chemosphere, 2011, 84: 908
16. [16]
  [16] Guo D H, Deng X J, Zhao S Z, et al. Chinese Journal of Analytical Chemistry (郭德华, 邓晓军, 赵善贞, 等. 分析化学), 2010, 38(3): 318
17. [17]
  [17] European Union (2002/657/EC)
18. [18]
  [18] Angelika W, Marek B. Food Chem, 2011, 125: 803
19. [19]
  [19] George S, Timothy B. Anal Chim Acta, 2009, 637: 68
20. [20]
  [20] Xiang P, Shen M, Zhuo X Y. Journal of Instrumental Analysis (向平, 沈敏, 卓先义. 分析测试学报), 2009, 28(6): 753
21. [21]
  [21] Little J L, Wempe M F, Buchanan C M. J Chromatogr B, 2006, 833(2): 219
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