Citation: LI Aimei, HUANG Zhuo, LU Wenping, XU Zhongqi. Matrix effect and application of field-amplified sample injection in the analysis of four tetracyclines in waters by capillary electrophoresis[J]. Chinese Journal of Chromatography, ;2014, 32(8): 897-903. doi: 10.3724/SP.J.1123.2014.03044 shu

Matrix effect and application of field-amplified sample injection in the analysis of four tetracyclines in waters by capillary electrophoresis

LI Aimei ¹ ,
HUANG Zhuo ² ,
LU Wenping ² ,
XU Zhongqi ^1,3,,

1.
1. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China;

Corresponding author: XU Zhongqi,
Received Date: 27 March 2014
Available Online: 18 April 2014
Fund Project: 流域水资源与生态环境科学湖北省重点实验室开放研究基金项目(CKWV2013220/KY) (CKWV2013220/KY)国家自然科学基金项目(51379016). (51379016)

The system abilities of two chromatographic techniques, capillary electrophoresis (CE) and high performance liquid chromatography (HPLC), were compared for the analysis of four tetracyclines (tetracycline, chlorotetracycline, oxytetracycline and doxycycline). The pH, concentration of background electrolyte (BGE) were optimized for the analysis of the standard mixture sample, meanwhile, the effects of separation voltage and water matrix (pH value and hardness) effects were investigated. In hydrodynamic injection (HDI) mode, a good quantitative linearity and baseline separation within 9.0 min were obtained for the four tetracyclines at the optimal conditions; the analytical time was about half of that of HPLC. The limits of detection (LODs) were in the range of 0.28-0.62 mg/L, and the relative standard deviations (RSDs) (n=6) of migration time and peak area were 0.42%-0.56% and 2.24%-2.95%, respectively. The obtained recoveries spiked in tap water and fishpond water were at the ranges of 96.3%-107.2% and 87.1%-105.2%, respectively. In addition, the stacking method, field-amplified sample injection (FASI), was employed to improve the sensitivity, and the LOD was down to the range of 17.8-35.5 μg/L. With FASI stacking, the RSDs (n=6) of migration time and peak area were 0.85%-0.95% and 1.69%-3.43%, respectively. Due to the advantages of simple sample pretreatment and fast speed, CE is promising in the analysis of the antibiotics in environmental water.
- capillary electrophoresis (CE),
- field-amplified sample injection (FASI),
- tetracyclines (TCs),
- environmental water,
- matrix effect
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