Citation: ZHENG Ling, WU Yujie, ZHAO Yongfeng, LI Lihua, MA Yanjuan. Simultaneous determination of 18β-agonist residues in feed using QuEChERS sample preparation and high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(8): 867-873. doi: 10.3724/SP.J.1123.2014.03029 shu

Simultaneous determination of 18β-agonist residues in feed using QuEChERS sample preparation and high performance liquid chromatography-tandem mass spectrometry

1.
Guangxi Entry-Exit Inspection and Quarantine Bureau, Nanning 530021, China

Corresponding author: WU Yujie,
Received Date: 19 March 2014
Available Online: 22 April 2014
Fund Project: 广西科技厅2012重大专项课题(桂科重:1222003-2-1). (桂科重:1222003-2-1)

A multi-residue method was developed for the simultaneous determination of 18 β-agonist residues (clenbuterol, ractopamine, penbutolol, tulobuterol, etc) in feed by using QuEChERS sample preparation and high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The feed samples were dispersed by water, then the analytes were extracted with acetonitrile containing 4%(v/v) ammonia and cleaned up by QuEChERS method with 25 mg octadecylsilyl (C₁₈) and 50 mg primary secondary amine (PSA) adsorbents. The separation of compounds was carried on an Agilent ZORBAX Eclipse XDB-C₁₈ column (50 mm×4.6 mm, 1.8 μm) by a gradient elution using methanol-0.1%(v/v) formic acid aqueous solution as mobile phase. The analytes were detected by tandem mass spectrometry under multiple reaction monitoring (MRM) mode with positive electrospray ionization (ESI⁺) and quantified by the matrix-matched external standard method. The results showed that the calibration curves of the 18 β-agonists were linear in the range of 5-200 μg/L with correlation coefficients of 0.9912-0.9995. The average recoveries of the 18 analytes at three spiked levels of 0.05, 0.1 and 0.5 mg/kg ranged from 78.4% to 107.1% with the relative standard deviations (RSDs) of 3.5%-12.3%. The limit of quantification (LOQ, S/N≥10) was 0.05 mg/kg for each analyte . The developed method is simple and sensitive, and can be applied as a screen and confirmatory method for the analysis of β-agonists in feed.
- QuEChERS,
- high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),
- β -agonists,
- feed
1. [1]
  [1] Guo W, Yang L J, Shi W C, et al. Chinese Journal of Analysis Laboratory (郭伟, 杨丽君, 时文春, 等. 分析试验室), 2010, 29(4): 92
2. [2]
  [2] Teng N Y, Liu X H, He S H, et al. Chinese Journal of Health Laboratory Technology (滕南雁, 刘向红, 何颂华, 等. 中国卫生检验杂志), 2011, 21(7): 1617
3. [3]
  [3] Ministry of Agriculture. No. 176 Bulletin of the Ministry of Agriculture of the People's Republic of China (农业部. 中华人民共和国农业部公告第176号). [2002-02-09]. http://www.moa.gov.cn/zwllm/tzgg/gg/201104/t20110422_1976307.htm
4. [4]
  [4] Ministry of Agriculture. No. 1519 Bulletin of the Ministry of Agriculture of the People's Republic of China (农业部. 中华人民共和国农业部公告第1519号). [2010-12-27]. http://www.moa.gov.cn/zwllm/tzgg/gg/201104/t20110422_1976294.htm
5. [5]
  [5] Liu X G, Xu J, Dong F S, et al. Anal Bioanal Chem, 2011, 401(3): 1051
6. [6]
  [6] Wang J, Chow W, Cheung W. J Agric Food Chem, 2011, 59: 8589
7. [7]
  [7] Xu J, Chen J, Ye H Y, et al. Journal of Instrumental Analysis (徐娟, 陈捷, 叶弘毅, 等. 分析测试学报), 2011, 30(9): 990
8. [8]
  [8] Xu J, Chen J, Wang L, et al. Journal of Instrumental Analysis (徐娟, 陈捷, 王岚, 等. 分析测试学报), 2013, 32(3): 293
9. [9]
  [9] Zhang Z Y, Gong Y, Shan W L, et al. Chinese Journal of Chromatography (张志勇, 龚勇, 单炜力, 等. 色谱), 2012, 30(1): 91
10. [10]
  [10] Wang L Z, Zhou Y, Chen Y, et al. Chinese Journal of Chromatography (王连珠, 周昱, 陈泳, 等. 色谱), 2012, 30(2): 146
11. [11]
  [11] Sung W L, Jeong H C, Soon K C, et al. J Chromatogr A, 2011, 1218: 4366
12. [12]
  [12] Ondrej L, Jana U, Jan P, et al. J Chromatogr A, 2010, 1217: 648
13. [13]
  [13] Lan F, Zhang Y, Yue Z F, et al. Journal of Instrumental Analysis (蓝方, 张毅, 岳振峰, 等. 分析测试学报), 2012, 31(12): 1471
14. [14]
  [14] Luo H T, Huang X L, Wu H Q, et al. Journal of Instrumental Analysis (罗辉泰, 黄晓兰, 吴惠勤, 等. 分析测试学报), 2011, 30(12): 1329
15. [15]
  [15] Bu M N, Shi Z H, Kang J, et al. Journal of Instrumental Analysis (卜明楠, 石志红, 康健, 等. 分析测试学报), 2012, 31(5): 552
16. [16]
  [16] Wang L, Li Y Q, Zhou Y K. Chinese Journal of Analysis Laboratory (王炼, 黎源倩, 周运柯. 分析试验室), 2009, 28(3): 78
17. [17]
  [17] Wang P L, Fan L, Su X O, et al. Chinese Journal of Analytical Chemistry (王培龙, 范理, 苏晓鸥, 等. 分析化学), 2012, 40(3): 470
18. [18]
  [18] Lu Y F, Xu L J, Wang H, et al. Journal of Chinese Mass Spectrometry Society (卢艳芬, 徐丽君, 王辉, 等. 质谱学报), 2012, 33(5): 280
19. [19]
  [19] Cai Q R, Wu J S, Qian Z J, et al. Chinese Journal of Chromatography (蔡勤仁, 吴洁珊, 钱振杰, 等. 色谱), 2013, 31(3): 200
20. [20]
  [20] Wan Y P, Liu N, Tao G C, et al. Journal of Henan Agricultural Science (万宇平, 刘宁, 陶光灿, 等. 河南农业科学), 2013, 42(2): 132
21. [21]
  [21] No. 1029-1-2011 Bulletin of the Ministry of Agriculture, National Standards of the People's Republic of China (农业部1029号公告-1-2011, 中华人民共和国国家标准)
22. [22]
  [22] No. 1486-1-2010 Bulletin of the Ministry of Agriculture, National Standards of the People's Republic of China (农业部1486号公告-1-2010, 中华人民共和国国家标准)
23. [23]
  [23] Li D N, Yan F, Zhang W G, et al. Feed Research (李丹妮, 严凤, 张文刚, 等. 饲料研究), 2011(12): 43
24. [24]
  [24] Tso J, Aga D S. J Chromatogr A, 2010, 1217: 4784
25. [25]
  [25] Liu Y T, Ai X H, Yang H. Journal of Instrumental Analysis (刘永涛, 艾晓辉, 杨红. 分析测试学报), 2011, 30(6): 640
26. [26]
  [26] Zheng L, Wu Y J, Li Y, et al. Chinese Journal of Chromatography (郑玲, 吴玉杰, 李湧, 等. 色谱), 2012, 30(7): 660
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