Citation: ZHANG Wenhua, HUANG Chaoqun, XIE Wen, SHEN Li. Determination of gossypol in edible vegetable oil with high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(6): 582-585. doi: 10.3724/SP.J.1123.2014.02029 shu

Determination of gossypol in edible vegetable oil with high performance liquid chromatography-tandem mass spectrometry

ZHANG Wenhua ¹ ,
HUANG Chaoqun ¹ ,
XIE Wen ^1,, ,
SHEN Li ²

1.
1. Zhejiang Entry-Exit Inspection and Quarantine Bureau, Hangzhou 310016, China;

Corresponding author: XIE Wen,
Received Date: 28 February 2014
Available Online: 4 April 2014
Fund Project: 出入境检验检疫行业标准制(修)订项目(2012B308r). (修)订项目(2012B308r)

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the determination of gossypol in edible vegetable oil. The sample was extracted with ethyl alcohol by vortex-excited oscillation. The extract was cleaned up by 0.22 μm filter membrane and centrifuged for 5 min at 4 000 r/min after standing in a fridge at 4 ℃ for 30 min. The compound was separated on a C18 column (100 mm×2.1 mm, 3.5 μm) with acetonitrile and 1% (v/v) formic acid aqueous solution as mobile phase. The detection of gossypol was carried out by LC-MS/MS with positive electrospray ionization under multiple reaction monitoring (MRM) mode using external standard method. The limits of quantification (S/N>10) of gossypol in edible vegetable oil was 1 mg/kg. The recoveries were from 87.4% to 100% at the spiked levels of 1, 2, 200 mg/kg of gossypol in edible vegetable oil with the relative standard deviations (RSDs) between 3.9% and 12.2%. The method, with high sensitivity, good precision and high recovery, was suitable for the confirmation and quantification of gossypol residue in edible vegetable oil.
- liquid chromatography-tandem mass spectrometry (LC-MS/MS),
- gossypol,
- edible oil
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