Citation: ZHENG Hao, MAO Jiawei, PAN Yanbo, LIU Zhongshan, LIU Zheyi, YE Mingliang, ZOU Hanfa. Effect of the lysine guanidination on proteomic analysis[J]. Chinese Journal of Chromatography, ;2014, 32(4): 376-380. doi: 10.3724/SP.J.1123.2014.01003 shu

Effect of the lysine guanidination on proteomic analysis

ZHENG Hao ^1,2 ,
MAO Jiawei ^1,2 ,
PAN Yanbo ^1,2 ,
LIU Zhongshan ^1,2 ,
LIU Zheyi ^1,2 ,
YE Mingliang ^1,, ,
ZOU Hanfa ^1,,

1.
1. Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;

Corresponding author: YE Mingliang, ZOU Hanfa,
Received Date: 3 January 2014
Available Online: 15 January 2014
Fund Project: 国家自然科学基金委员会创新研究群体科学基金(21321064). (21321064)

The guanidination of lysine side chain was paid great attention in recent years. It plays an important role in qualitative and quantitative proteomics. In this study, based on the results of separated peptides extracted from HeLa cells before and after the guanidination by liquid chromatography-tandem mass spectrometry (LC-MS/MS), the effect of the guanidination of three different kinds of peptides was systematically analyzed. It was found that the selectivity of the guanidination of the lysine side chain was as high as 96.8%. The ratio of identified peptides with lysine at C-term to all peptides increased from 51.7% to 57.3% and more new peptides were identified, while the ratio of peptides with lysine in the middle or without lysine changed little. Further study on the ratio of b and y ions indicated that there were more y ions of peptides with lysine at C-term after the guanidination. The results proved that the selective conversion of lysine to homoarginine by the guanidination could increase the sensitivity and selectivity of mass spectrum. The increased basicity and ability to sequester proton of lysine produced more y ions fragmentation information, which contributed to more identified peptides. It concluded that the lysine guanidination can improve the coverage of proteomic analysis.
- mass spectrometry,
- proteomics,
- guanidination,
- peptide fragmentation
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