Citation: LI Zhenyu, FU Qing, LI Kuiyong, LIANG Tu, JIN Yu. Fast analysis of indole alkaloids from Evodiae fructus by supercritical fluid chromatography[J]. Chinese Journal of Chromatography, ;2014, 32(5): 506-512. doi: 10.3724/SP.J.1123.2013.12035 shu

Fast analysis of indole alkaloids from Evodiae fructus by supercritical fluid chromatography

1.
School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China

Corresponding author: JIN Yu,
Received Date: 30 December 2013
Available Online: 27 February 2014
Fund Project: 国家自然科学基金项目(21005028) (21005028)华东理工大学基本科研业务费探索研究基金(WY1214070). (WY1214070)

A fast chromatographic separation of indole alkaloids from Evodiae fructus was developed by supercritical fluid chromatography (SFC). The initial screening of four stationary phases was investigated with a standard mixture of evodiamine and rutaecarpine, and a complex sample of indole alkaloids prepared from Evodiae fructus as probes. Later, the effects of chromatographic parameters on separation were studied including injection volume, organic modifier, additive, temperature and back pressure. The injection volume had significant impact on the peak shape. With the additives in the mobile phase, slight changes in peak shape and retention time were observed in separation. Variation in organic modifier led to dramatic change in chromatographic behavior. Both decreased temperature and increased back pressure shortened the retention time. Finally, a fast analytical method using SFC, on a Waters ACQUITY UPC² BEH column, methanol as modifier, under 35 ℃ and 2.07×10⁷ Pa, was developed to separate a complex sample of indole alkaloids in less than 15 min. Another rapid approach for the separation of a complex sample of indole alkaloids was developed by using ultra-high performance liquid chromatography (UHPLC). As a result, SFC can be used in the separation of natural products, giving high performance, good resolution and fast analysis speed. The difference in selectivity with UHPLC can be used to the development of natural product separation.
- supercritical fluid chromatography (SFC),
- indole alkaloids,
- Evodiae fructus,
- fast analysis,
- ultra-high performance liquid chromatography (UHPLC)
1. [1]
  [1] Yang C L, Hu H Q, Zhang W B, et al. Journal of Analytical Science (杨长龙, 胡浩权, 张维冰, 等. 分析科学学报), 2005, 21(4): 444
2. [2]
  [2] Guo Y D. Natural Product Research and Development (郭亚东. 天然产物研究与开发), 2001, 13(3): 61
3. [3]
  [3] Zhang Y P, Hong Z, Fang H, et al. Guiding Journal of Traditional Chinese Medicine and Pharmacology (张怡评, 洪专, 方华, 等. 中医药导报), 2012, 18(7): 89
4. [4]
  [4] Wang X J, Zhao S Q, Wang R A. Chinese Journal of Chromatography (王学军, 赵锁奇, 王仁安. 色谱), 2001, 19(4): 344
5. [5]
  [5] Xu X F, Shen A J, Guo Z M, et al. Chinese Journal of Chromatography (徐雪峰, 沈爱金, 郭志谋, 等. 色谱), 2013, 31(3): 185
6. [6]
  [6] Taylor L T. LCGC North America, 2009: 490. (2009-06-01). http://www.chromatographyonline.com/lcgc/Misc/Separation-of-Ionic-Analytes-via-Supercritical-Flu/ArticleStandard/Article/detail/605649
7. [7]
  [7] Miller L. J Chromatogr A, 2012, 1250: 250
8. [8]
  [8] Zhang J Z, Wang Y, Chen H, et al. Spectroscopy and Spectral Analysis (张进治, 汪瑗, 陈惠, 等. 光谱学与光谱分析), 2007, 27(5): 944
9. [9]
  [9] Zhang X Q, Zhou X, Wang D P, et al. Chinese Journal of Analytical Chemistry (张雪琴, 周欣, 王道平, 等. 分析化学), 2005, 33(2): 241
10. [10]
  [10] Sun J, Baker A, Chen P. Rapid Commun Mass Spectrom, 2011, 25(18): 2591
11. [11]
  [11] Okunji C O, Iwu M M, Ito Y, et al. J Liq Chromatogr Rel Technol, 2005, 28(5): 775
12. [12]
  [12] Liu R M, Chu X, Sun A L, et al. J Chromatogr A, 2005, 1074: 139
13. [13]
  [13] Yang M, Zhou P, Xu L, et al. Journal of Instrumental Analysis (杨敏, 周萍, 徐路, 等. 分析测试学报), 2010, 29(7): 743
14. [14]
  [14] Qiu S Y. [MS Dissertation]. Shanghai: East China University of Science and Technology (邱时祎. [硕士学位论文]. 上海: 华东理工大学), 2013
15. [15]
  [15] Perrenoud A G G, Boccard J, Veuthey J L, et al. J Chromatogr A, 2012, 1262: 205
16. [16]
  [16] Chou F M, Wang W T, Wei G T. J Chromatogr A, 2009, 1216: 3594
17. [17]
  [17] Zhang Y, Du Z X, Yu W L. Chinese Journal of Chromatography (张云, 杜振霞, 于文莲. 色谱), 2014, 32(1): 52
18. [18]
  [18] West C, Bouet A, Routier S, et al. J Chromatogr A, 2012, 1269: 325
1. [1]
  Zhuomin Zhang , Hanbing Huang , Liangqiu Lin , Jingsong Liu , Gongke Li . Course Construction of Instrumental Analysis Experiment: Surface-Enhanced Raman Spectroscopy for Rapid Detection of Edible Pigments. University Chemistry, 2024, 39(2): 133-139. doi: 10.3866/PKU.DXHX202308034
2. [2]
  Zhenjun Mao , Haorui Gu , Haiyan Che , Xufeng Lin . Exploration on Experiment Teaching of UHPLC-IC Based on Valve Switching Method. University Chemistry, 2024, 39(4): 81-86. doi: 10.3866/PKU.DXHX202311013
3. [3]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
4. [4]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
5. [5]
  Jingming Li , Bowen Ding , Nan Li , Nurgul . Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching. University Chemistry, 2024, 39(8): 263-269. doi: 10.3866/PKU.DXHX202312078
6. [6]
  Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120
7. [7]
  Yurong Tang , Yunren Shi , Yi Xu , Bo Qin , Yanqin Xu , Yunfei Cai . Innovative Experiment and Course Transformation Practice of Visible-Light-Mediated Photocatalytic Synthesis of Isoquinolinone. University Chemistry, 2024, 39(5): 296-306. doi: 10.3866/PKU.DXHX202311087
8. [8]
  Jingjie Tang , Luying Xie , Jiayu Liu , Shangyu Shi , Xinyu Sun , Jiayang Lin , Qikun Yang , Chuan'ang Yu , Zecheng Wang , Yingying Wang , Zengyang Xie . Efficient Rapid Synthesis and Antibacterial Activities of Tosylhydrazones: A Recommended Innovative Chemistry Experiment for Undergraduate Medical University. University Chemistry, 2024, 39(3): 316-326. doi: 10.3866/PKU.DXHX202309091
9. [9]
  . . Chinese Journal of Inorganic Chemistry, 2024, 40(12): 0-0.
10. [10]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
11. [11]
  Di Yang , Jiayi Wei , Hong Zhai , Xin Wang , Taiming Sun , Haole Song , Haiyan Wang . Rapid Detection of SARS-CoV-2 Using an Innovative “Magic Strip”. University Chemistry, 2024, 39(4): 373-381. doi: 10.3866/PKU.DXHX202312023
12. [12]
  Zhibei Qu , Changxin Wang , Lei Li , Jiaze Li , Jun Zhang . Organoid-on-a-Chip for Drug Screening and the Inherent Biochemistry Principles. University Chemistry, 2024, 39(7): 278-286. doi: 10.3866/PKU.DXHX202311039
13. [13]
  Conghao Shi , Ranran Wang , Juli Jiang , Leyong Wang . The Illustration on Stereoisomers of Macrocycles Containing Multiple Chiral Centers via Tröger Base-based Macrocycles. University Chemistry, 2024, 39(7): 394-397. doi: 10.3866/PKU.DXHX202311034
14. [14]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
15. [15]
  Xin MA , Ya SUN , Na SUN , Qian KANG , Jiajia ZHANG , Ruitao ZHU , Xiaoli GAO . A Tb₂ complex based on polydentate Schiff base: Crystal structure, fluorescence properties, and biological activity. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1347-1356. doi: 10.11862/CJIC.20230357
16. [16]
  Jinlong YAN , Weina WU , Yuan WANG . A simple Schiff base probe for the fluorescent turn-on detection of hypochlorite and its biological imaging application. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1653-1660. doi: 10.11862/CJIC.20240154
17. [17]
  Zhenli Sun , Ning Wang , Kexin Lin , Qin Dai , Yufei Zhou , Dandan Cao , Yanfeng Dang . Visual Analysis of Hotspots and Development Trends in Analytical Chemistry Education Reform. University Chemistry, 2024, 39(11): 57-64. doi: 10.12461/PKU.DXHX202403095
18. [18]
  Zhaoyang Li , Haiyan Zhao , Yali Zhang , Yuan Zhang , Shiqiang Cui . Integration of Nobel Prize Achievements in Analytical Technology with College Instrumental Analysis Course. University Chemistry, 2025, 40(3): 269-276. doi: 10.12461/PKU.DXHX202405131
19. [19]
  Yikai Wang , Xiaolin Jiang , Haoming Song , Nan Wei , Yifan Wang , Xinjun Xu , Cuihong Li , Hao Lu , Yahui Liu , Zhishan Bo . 氰基修饰的苝二酰亚胺衍生物作为膜厚不敏感型阴极界面材料用于高效有机太阳能电池. Acta Physico-Chimica Sinica, 2025, 41(3): 2406007-. doi: 10.3866/PKU.WHXB202406007
20. [20]
  Zhening Lou , Quanxing Mao , Xiaogeng Feng , Lei Zhang , Xu Xu , Yuyang Zhang , Xueyan Liu , Hongling Kang , Dongyang Feng , Yongku Li . Practice of Implementing Blended Teaching in Shared Analytical Chemistry Course. University Chemistry, 2024, 39(2): 263-269. doi: 10.3866/PKU.DXHX202308089

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(472)
HTML views(54)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142