Citation: XIE Hanbing, ZHOU Mingying, ZHAO Haifeng, WANG Yigang, JIANG Wanfeng, ZHAO Shan. Determination of three exogenous plant hormone residues in bean sprout by high performance liquid chromatography-quadrupole-time of flight mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(5): 493-498. doi: 10.3724/SP.J.1123.2013.12029 shu

Determination of three exogenous plant hormone residues in bean sprout by high performance liquid chromatography-quadrupole-time of flight mass spectrometry

1.
1. Qingdao Quality Supervision and Inspection Center for Shopping Basket Commodity, Qingdao 266071, China;

Corresponding author: ZHOU Mingying,
Received Date: 25 December 2013
Available Online: 26 February 2014
Fund Project: 青岛市2013年“菜篮子”工程建设资助项目(青菜篮子[2013]1号). (青菜篮子[2013]1号)

This study was aimed to the establishment of an analytical method for the determination of three exogenous plant hormone residues in bean sprout by high performance liquid chromatography-quadrupole-time of flight mass spectrometry (HPLC-Q-TOF-MS). The target compounds were gibberellins, 6-benzylaminopurine and parachlorophenoxyacetic acid. The QuEChERS (quick, easy, cheap, effective, rugged, and safe) method was used for sample preparation. The analytes were extracted with a solution containing 1% (v/v, if not specified) acetic, 50% ethanol, 49% acetonitrile, and cleaned-up by dispersive solid-phase extraction with diatomite dispersant, then degreased by hexane. The three target compounds were separated on an Eclipse Plus C₁₈ column (100 mm×3.0 mm, 1.8 μm) with mobile phases A (water containing 0.1% formic acid) and B (methanol) by gradient elution within 15 min, and detected under negative electrospray ionization (ESI) mode. The quantitative analysis was carried out by extracting the peak area with accurate mass. The confirmatory analysis of the target compounds was performed with the qualitative fragments. The results showed that the limits of quantification (LOQs, S/N=10) for the three target compounds were from 5.0 μg/kg to 10 μg/kg. The respective mean recoveries were found to be in the range of 79.1%-96.1%, and the RSDs were 5.7%-10.4%. It was applicable to the analysis of the three exogenous plant hormones in bean sprout samples. This method is simple, fast and efficient.
1. [1]
  [1] Geng G C, Pan Q M, Qian G W. Jiangsu Chemical Industry (耿国彩, 潘启民, 钱光伟. 江苏化工), 1995, 23(4): 47
2. [2]
  [2] Feng J W, Wu J S, Cao G Y, et al. Physical and Chemical Testing Part B: Chemical Analysis (冯家望, 吴洁珊, 曹桂云, 等. 理化检验: 化学分册), 2012, 48(10): 1219
3. [3]
  [3] Ding J Z, Yin T, Yu X, et al. Plant Physiology Journal (丁俊胄, 尹涛, 余翔, 等. 植物生理学报), 2011, 47(5): 501
4. [4]
  [4] Xie J, Zhang Y Z. Journal of Instrumental Analysis (谢君, 张义正. 分析测试学报), 2001, 20(1): 60
5. [5]
  [5] Jin M C, Chen X H, Li X P, et al. Analytical Instrumentation (金米聪, 陈晓红, 李小平, 等. 分析仪器), 2005(3): 29
6. [6]
  [6] DB11/T 379-2006
7. [7]
  [7] DBS22/001-2013
8. [8]
  [8] GB/T 23381-2009
9. [9]
  [9] Lin L. The Food Industry (林琳. 食品工业), 2008(3): 65
10. [10]
  [10] Huang W P. China Preventive Medicine (黄卫平. 中华预防医学杂志), 2002, 36(1): 44
11. [11]
  [11] Peng S, Zhang H, Huan Z Y, et al, Shandong Agricultural Sciences (彭姝, 张昊, 郇正玉, 等. 山东农业科学), 2010(2): 95
12. [12]
  [12] Li X P, Chen X H, Yao X P, et al. Chinese Journal of Health Laboratory Technology (李小平, 陈晓红, 姚浔平, 等. 中国卫生检验), 2005, 15(2): 149
13. [13]
  [13] Xie H B, Lin H, Jiang W F, et al. Shandong Agricultural Sciences (谢寒冰, 林洪, 蒋万枫, 等. 山东农业科学), 2008(2): 92
14. [14]
  [14] Liu H, Wu B, Yin Y, et al. Chinese Journal of Chromatography (柳菡, 吴斌, 殷耀, 等. 色谱), 2013, 31(1): 22
15. [15]
  [15] DB33/T 625.3-2007
16. [16]
  [16] SN/T 0350-2012
17. [17]
  [17] Kuang H, Hou Y X, Chu X G, et al. Chinese Journal of Analytical Chemistry (匡华, 侯玉霞, 储晓刚, 等. 分析化学), 2006, 34(12): 1733
18. [18]
  [18] Yan J L, Yan Y Q, Wang L, et al. Chinese Journal of Health Laboratory Technology (颜金良, 颜勇卿, 王立, 等. 中国卫生检验杂志), 2006, 16(10): 1027
19. [19]
  [19] Ding Y C, Jiang A X, Zhong M W. Chinese Journal of Public Health (丁友昌, 姜爱香, 钟鸣文. 中国公共卫生), 1997, 13(7): 77
20. [20]
  [20] Lan F, Zhang Y, Yue Z F, et al. Journal of Instrumental Analysis (蓝芳, 张毅, 岳振峰, 等. 分析测试学报), 2012, 31(12): 1471
21. [21]
  [21] Xiang P, Shen M, Zhuo X Y. Journal of Instrumental Analysis (向平, 沈敏, 卓先义. 分析测试学报), 2009, 28(6): 753
22. [22]
  [22] Wang L Q, He L M, Zeng Z L, et al. Journal of Chinese Mass Spectrometry Society (王立琦, 贺利民, 曾振灵, 等. 质谱学报), 2011, 32(6): 321
1. [1]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
2. [2]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
3. [3]
  Xiaofeng Xia , Jielian Zhu . Innovative Comprehensive Experimental Design: Synthesis of 6-Fluoro-N-benzoyl Tetrahydroquinoline. University Chemistry, 2024, 39(10): 344-352. doi: 10.12461/PKU.DXHX202405063
4. [4]
  Pengcheng Yan , Peng Wang , Jing Huang , Zhao Mo , Li Xu , Yun Chen , Yu Zhang , Zhichong Qi , Hui Xu , Henan Li . Engineering Multiple Optimization Strategy on Bismuth Oxyhalide Photoactive Materials for Efficient Photoelectrochemical Applications. Acta Physico-Chimica Sinica, 2025, 41(2): 100014-. doi: 10.3866/PKU.WHXB202309047
5. [5]
  Yi DING , Peiyu LIAO , Jianhua JIA , Mingliang TONG . Structure and photoluminescence modulation of silver(Ⅰ)-tetra(pyridin-4-yl)ethene metal-organic frameworks by substituted benzoates. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 141-148. doi: 10.11862/CJIC.20240393
6. [6]
  Peiling Li , Qing Feng , Hongling Yuan , Qin Wang . Live Interview Recording about the Penicillin Family. University Chemistry, 2024, 39(9): 122-127. doi: 10.3866/PKU.DXHX202311022
7. [7]
  Meijin Li , Xirong Fu , Xue Zheng , Yuhan Liu , Bao Li . The Marvel of NAD⁺: Nicotinamide Adenine Dinucleotide. University Chemistry, 2024, 39(9): 35-39. doi: 10.12461/PKU.DXHX202401027
8. [8]
  Jingzhao Cheng , Shiyu Gao , Bei Cheng , Kai Yang , Wang Wang , Shaowen Cao . 4-氨基-1H-咪唑-5-甲腈修饰供体-受体型氮化碳光催化剂的构建及其高效光催化产氢研究. Acta Physico-Chimica Sinica, 2024, 40(11): 2406026-. doi: 10.3866/PKU.WHXB202406026
9. [9]
  Endong YANG , Haoze TIAN , Ke ZHANG , Yongbing LOU . Efficient oxygen evolution reaction of CuCo₂O₄/NiFe-layered bimetallic hydroxide core-shell nanoflower sphere arrays. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 930-940. doi: 10.11862/CJIC.20230369
10. [10]
  Hong CAI , Jiewen WU , Jingyun LI , Lixian CHEN , Siqi XIAO , Dan LI . Synthesis of a zinc-cobalt bimetallic adenine metal-organic framework for the recognition of sulfur-containing amino acids. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 114-122. doi: 10.11862/CJIC.20240382
11. [11]
  Jingjie Tang , Luying Xie , Jiayu Liu , Shangyu Shi , Xinyu Sun , Jiayang Lin , Qikun Yang , Chuan'ang Yu , Zecheng Wang , Yingying Wang , Zengyang Xie . Efficient Rapid Synthesis and Antibacterial Activities of Tosylhydrazones: A Recommended Innovative Chemistry Experiment for Undergraduate Medical University. University Chemistry, 2024, 39(3): 316-326. doi: 10.3866/PKU.DXHX202309091
12. [12]
  Zhongxin YU , Wei SONG , Yang LIU , Yuxue DING , Fanhao MENG , Shuju WANG , Lixin YOU . Fluorescence sensing on chlortetracycline of a Zn-coordination polymer based on mixed ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2415-2421. doi: 10.11862/CJIC.20240304
13. [13]
  Xiuyun Wang , Jiashuo Cheng , Yiming Wang , Haoyu Wu , Yan Su , Yuzhuo Gao , Xiaoyu Liu , Mingyu Zhao , Chunyan Wang , Miao Cui , Wenfeng Jiang . Improvement of Sodium Ferric Ethylenediaminetetraacetate (NaFeEDTA) Iron Supplement Preparation Experiment. University Chemistry, 2024, 39(2): 340-346. doi: 10.3866/PKU.DXHX202308067
14. [14]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
15. [15]
  Gaofeng WANG , Shuwen SUN , Yanfei ZHAO , Lixin MENG , Bohui WEI . Structural diversity and luminescence properties of three zinc coordination polymers based on bis(4-(1H-imidazol-1-yl)phenyl)methanone. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 849-856. doi: 10.11862/CJIC.20230479
16. [16]
  Yongqing Kuang , Jie Liu , Jianjun Feng , Wen Yang , Shuanglian Cai , Ling Shi . Experimental Design for the Two-Step Synthesis of Paracetamol from 4-Hydroxyacetophenone. University Chemistry, 2024, 39(8): 331-337. doi: 10.12461/PKU.DXHX202403012
17. [17]
  Di Yang , Jiayi Wei , Hong Zhai , Xin Wang , Taiming Sun , Haole Song , Haiyan Wang . Rapid Detection of SARS-CoV-2 Using an Innovative “Magic Strip”. University Chemistry, 2024, 39(4): 373-381. doi: 10.3866/PKU.DXHX202312023
18. [18]
  Xiyuan Su , Zhenlin Hu , Ye Fan , Xianyuan Liu , Xianyong Lu . Change as You Want: Multi-Responsive Superhydrophobic Intelligent Actuation Material. University Chemistry, 2024, 39(5): 228-237. doi: 10.3866/PKU.DXHX202311059
19. [19]
  Zongpei Zhang , Yanyang Li , Yanan Si , Kai Li , Shuangquan Zang . Developing a Chemistry Experiment Center Employing a Multifaceted Approach to Serve High-Quality Laboratory Education. University Chemistry, 2024, 39(7): 13-19. doi: 10.12461/PKU.DXHX202404041
20. [20]
  Ruilin Han , Xiaoqi Yan . Comparison of Multiple Function Methods for Fitting Surface Tension and Concentration Curves. University Chemistry, 2024, 39(7): 381-385. doi: 10.3866/PKU.DXHX202311023

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(586)
HTML views(7)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142