Citation: LI Zhaoyong, WANG Fengmei, NIU Zengyuan, LUO Xin, ZHANG Gang, CHEN Junhui. Screening and confirmation of 24 hormones in cosmetics by ultra high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(5): 477-484. doi: 10.3724/SP.J.1123.2013.12004 shu

Screening and confirmation of 24 hormones in cosmetics by ultra high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry

LI Zhaoyong ^1,2 ,
WANG Fengmei ² ,
NIU Zengyuan ^2,, ,
LUO Xin ² ,
ZHANG Gang ² ,
CHEN Junhui ¹

1.
1. Research Center for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China;

Corresponding author: NIU Zengyuan,
Received Date: 9 December 2013
Available Online: 13 January 2014
Fund Project: 国家质检总局科研项目(2011IK224,2011IK037). (2011IK224,2011IK037)

A method of ultra high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry (UPLC-LTQ/Orbitrap MS) was established to screen and confirm 24 hormones in cosmetics. Various cosmetic samples were extracted with methanol. The extract was loaded onto a Waters ACQUITY UPLC BEH C18 column (50 mm×2.1 mm, 1.7 μm) using a gradient elution of acetonitrile/water containing 0.1%(v/v) formic acid for the separation. The accurate mass of quasi-molecular ion was acquired by full scanning of electrostatic field orbitrap. The rapid screening was carried out by the accurate mass of quasi-molecular ion. The confirmation analysis for targeted compounds was performed with the retention time and qualitative fragments obtained by data dependent scan mode. Under the optimal conditions, the 24 hormones were routinely detected with mass accuracy error below 3×10^-6(3 ppm), and good linearities were obtained in their respective linear ranges with correlation coefficients higher than 0.99. The LODs (S/N=3) of the 24 compounds were ≤10 μg/kg, which can meet the requirements for the actual screening of cosmetic samples. The developed method was applied to screen the hormones in 50 cosmetic samples. The results demonstrate that the method is a useful tool for the rapid screening and identification of the hormones in cosmetics.
- ultra high performance liquid chromatography (UPLC),
- linear ion trap/orbitrap mass spectrometry (LTQ/Orbitrap MS),
- hormones,
- cosmetics
1. [1]
  [1] Plotan M, Frizzell C, Robinson V, et al. Food Chem, 2013, 136(3/4): 1590
2. [2]
  [2] Regulation (EC) No. 1223/2009
3. [3]
  [3] Ministry of Health of the People’s Republic of China. Hygienic Standard for Cosmetics. Beijing: Ministry of Health of the People’s Republic of China (中华人民共和国卫生部. 化妆品卫生规范. 北京: 中华人民共和国卫生部), 2007
4. [4]
  [4] Li J, Zheng H Y, Wang L N, et al. J Eur Acad Dermatol Venereol, 2009, 23(6): 648
5. [5]
  [5] Liu J. Chinese Journal of Health Laboratory Technology (刘军. 中国卫生检验杂志), 2000, 10(5): 571
6. [6]
  [6] Cullen P A, Cameron C E. Expert Rev Vaccines, 2006, 5(1): 67
7. [7]
  [7] Ma Q, Wang C, Wang X, et al. Chinese Journal of Chromatography (马强, 王超, 王星, 等. 色谱), 2007, 25(4): 541
8. [8]
  [8] Jin W, Yang Y J, Wang W Y, et al. Chromatographia, 2009, 69: 1221
9. [9]
  [9] Dio T, Kajimura K, Takatori S, et al. J Chromatogr B, 2009, 877: 1005
10. [10]
  [10] Zhao X Y, Lin Y F, Hu X Z, et al. Chinese Journal of Analysis Laboratory (赵晓亚, 林雁飞, 胡小钟, 等. 分析试验室), 2009, 28(2): 111
11. [11]
  [11] Wang C, Ma Q, Wang X, et al. Chinese Journal of Analytical Chemistry (王超, 马强, 王星, 等. 分析化学), 2007, 35(9): 1257
12. [12]
  [12] Huang B F, Ye L, Han J L, et al. Chinese Journal of Health Laboratory Technology (黄百芬, 叶蕾, 韩建龙, 等. 中国卫生检验杂志), 2011, 21(5): 1048
13. [13]
  [13] Wu Z P, Lu J, Gao W, et al. China Surfactant Detergent & Cosmetics (武中平, 卢剑, 高巍, 等. 日用化学工业), 2010, 40(2): 153
14. [14]
  [14] Harwood D T, Handelsman D J. Clin Chim Acta, 2009, 409: 78
15. [15]
  [15] Wang X B, Ding L, Zhu S H, et al. Packaging Engineering (王晓兵, 丁利, 朱绍华, 等. 包装工程), 2011, 32(15): 43
16. [16]
  [16] Rao Y L, Xiang B R, Bramanti E, et al. J Agric Food Chem, 2010, 58: 1462
17. [17]
  [17] Li Y L, Zheng Y J, Xiong C, et al. Chinese Journal of Chromatography (黎永乐, 郑彦婕, 熊岑, 等. 色谱), 2013, 31(8): 729
18. [18]
  [18] Yun H, Liu X, Wang J, et al. Chinese Journal of Chromatography (云环, 刘鑫, 王静, 等. 色谱), 2013, 31(9): 845
19. [19]
  [19] Xiu X L, Luo X, Niu Z Y, et al. Chinese Journal of Chromatography (修晓丽, 罗忻, 牛增元, 等. 色谱), 2013, 31(10): 961
20. [20]
  [20] Pichler P, Kcher T, Holzmann J, et al. Anal Chem, 2010, 82: 6549
21. [21]
  [21] SANCO/12495/2011
22. [22]
  [22] 2002/657/EC
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