Citation: ZHAO Haixiang, LIU Haiping, YAN Zaoying. Analysis of sulfonamide residues in pork and chicken by high performance liquid chromatography coupled with solid-phase extraction using multiwalled carbon nanotubes as adsorbent[J]. Chinese Journal of Chromatography, ;2014, 32(3): 294-298. doi: 10.3724/SP.J.1123.2013.11041 shu

Analysis of sulfonamide residues in pork and chicken by high performance liquid chromatography coupled with solid-phase extraction using multiwalled carbon nanotubes as adsorbent

1.
Institute of Applied Chemistry, Hebei North University, Zhangjiakou 075000, China

Corresponding author: ZHAO Haixiang,
Received Date: 21 November 2013
Available Online: 8 January 2014
Fund Project: 河北北方学院应用化学创新团队项目(CXTD1306) (CXTD1306)河北北方学院创新人才培育基金(CXRC1302). (CXRC1302)

A multi-residue analytical method based on solid-phase extraction (SPE) with multi-walled carbon nanotubes (MWCNTs) as sorbent was developed. The determination of the sulfonamides (SAs) in pork and chicken was carried out by high performance liquid chromatography-ultraviolet detection (HPLC-UV). The clean-up conditions were optimized. The analytes were extracted by acetonitril and cleaned-up by MWCNTs SPE cartridge. The extract was re-dissolved with the Na₂HPO₄ buffer (pH 5.5-6.0) for loading, and was washed with acetone-hexane (5:95, v/v), then eluted with acetone-dichloromethane (1:1, v/v) from the column. The mobile phase used in the chromatographic separation consisted of a binary mixture of acetonitrile and 50 mmol/L NaH₂PO₄ with the volume ratio of 7:3. A wide linear range was 0.01-1.00 mg/L with the correlation coefficients above 0.998. The limits of detection (S/N=3) were 0.003 mg/L, and the limits of quantification (S/N=10) were 0.01 mg/L. The average recoveries were over 70% for the nine SAs in the spiked range of 0.02-0.2 mg/kg, with the relative standard deviations (RSDs) lower than 8%. This study indicated that the MWCNTs SPE cartridge is efficient for the clean-up of the SAs in animal tissues or products, and the method is simple, accurate and suitable for the quantification of the SAs residues.
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沈阳化工大学材料科学与工程学院沈阳 110142