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Citation: ZHANG Yiding, CHANG Cuilan, GUO Qilei, CAO Hong, BAI Yu, LIU Huwei. Analysis of tartrazine aluminum lake and sunset yellow aluminum lake in foods by capillary zone electrophoresis[J]. Chinese Journal of Chromatography, ;2014, 32(4): 438-442. doi: 10.3724/SP.J.1123.2013.11020 shu

Analysis of tartrazine aluminum lake and sunset yellow aluminum lake in foods by capillary zone electrophoresis

ZHANG Yiding ¹ ,
CHANG Cuilan ¹ ,
GUO Qilei ² ,
CAO Hong ² ,
BAI Yu ^1,, ,
LIU Huwei ¹

1.
1. Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China;

Corresponding author: BAI Yu,
Received Date: 6 November 2013
Available Online: 31 December 2013
Fund Project: 北京市科技计划项目(Z111100056811006). (Z111100056811006)

A novel analytical method for tartrazine aluminum lake and sunset yellow aluminum lake using capillary zone electrophoresis (CZE) was studied. The pigments contained in the color lakes were successfully separated from the aluminum matrix in the pre-treatment process, which included the following steps: dissolve the color lakes in 0.1 mol/L H₂SO₄, adjust the pH of the solution to 5.0, then mix it with the solution of EDTA·52Na and heat it in a water bath, then use polyamide powder as the stationary phase of solid phase extraction to separate the pigments from the solution, and finally elute the pigments with 0.1 mol/L NaOH. The CZE conditions systematically optimized for tartrazine aluminum lake were: 48.50 cm of a fused silica capillary with 40.00 cm effective length and 50 μm i. d., the temperature controlled at 20.0 ℃, 29.0 kV applied, HPO₄^2--PO₄^3- (0.015 mol/L, pH 11.45) solution as running buffer, detection at 263 nm. The conditions for sunset yellow aluminum lake were: the same capillary and temperature, 25.0 kV applied, HPO₄^2--PO₄^3- (0.025 mol/L, pH 11.45) solution as running buffer, detection at 240 nm. The limits of detection were 0.26 mg/L and 0.27 mg/L, and the linear ranges were 0.53-1.3×10² mg/L and 0.54-1.4×10² mg/L for tartrazine aluminum lake and sunset yellow aluminum lake, respectively. The RSDs were 4.3% and 5.7% (run to run, n=6), 5.6% and 6.0% (day to day, n=6) for tartrazine aluminum lake and sunset yellow aluminum lake, respectively. Further developments for this method could make it a routinely used method analyzing color lakes in foods.
- capillary zone electrophoresis (CZE),
- tartrazine,
- sunset yellow,
- color lakes,
- foods
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