Citation: LUO Weiqiang, XIAO Xiaohua, DU Zhuo, LI Gongke. Monolith column solid-phase extraction coupled with high performance liquid chromatography in online mode for the determination of forchlorfenuron in fruits[J]. Chinese Journal of Chromatography, ;2014, 32(4): 407-412. doi: 10.3724/SP.J.1123.2013.11011 shu

Monolith column solid-phase extraction coupled with high performance liquid chromatography in online mode for the determination of forchlorfenuron in fruits

LUO Weiqiang ^1,2 ,
XIAO Xiaohua ^1,, ,
DU Zhuo ¹ ,
LI Gongke ^1,,

1.
1. School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China;

Corresponding author: XIAO Xiaohua, LI Gongke,
Received Date: 5 November 2013
Available Online: 12 December 2013
Fund Project: 国家自然科学基金项目(21127008)；高等学校博士学科点专项(20120171110001)；广州市科技计划项目(12A102051514). (21127008)；高等学校博士学科点专项(20120171110001)；广州市科技计划项目(12A102051514)

A novel method based on the monolithic column solid-phase extraction (SPE) coupled with high performance liquid chromatography in online mode was developed for the determination of trace forchlorfenuron in fruits. The enrichment factor of SPE for forchlorfenuron was 214. The method was fully validated. It showed a linear range of 0.01-50.00 μg/L and a limit of detection of 25 ng/L, with the relative standard deviation (RSD) of 3.9%. The proposed method was successfully applied for the determination of forchlorfenuron in grape, kiwifruit and watermelon samples, with the recoveries of 87.0%-120.7% and the RSDs of 0.6%-9.2%. This online method was proved to be selective, sensitive and convenient for the determination of trace forchlorfenuron in complex samples.
- monolith column,
- solid-phase extraction,
- high performance liquid chromatography (HPLC),
- online analysis,
- forchlorfenuron,
- fruits
1. [1]
  [1] Chen W J, Jiao B N, Su X S, et al. Bull Environ Contam Tox, 2013, 90: 756
2. [2]
  [2] Shi X M, Jin F, Huang Y T, et al. J Agric Food Chem, 2012, 60: 60
3. [3]
  [3] Shi X M, Jin F, Huang Y T, et al. Food Industry Science & Technology (史晓梅, 金芬, 黄玉婷, 等. 食品工业科技), 2012, 33(4): 417
4. [4]
  [4] Valverde A, Aguilera A, Ferrer C, et al. J Agric Food Chem, 2010, 58: 2818
5. [5]
  [5] Yang L, Lu J, Zhu J L, et al. Journal of Northwest Forestry University (杨柳, 陆军, 朱杰丽, 等. 西北林学院学报), 2013, 28(2): 156
6. [6]
  [6] Hu J T, Sheng Y, Fang Z, et al. Chinese Journal of Chromatography (胡江涛, 盛毅, 方智, 等. 色谱), 2007, 25(3): 441
7. [7]
  [7] Zhao L, Ma Q, Ma L. Journal of Analytical Science (赵莉, 马青, 马琳. 分析科学学报), 2013, 29(4): 583
8. [8]
  [8] Zhang J, Du P. Chinese Journal of Chromatography (张军, 杜平. 色谱), 2011, 29(11): 1133
9. [9]
  [9] Wang J, Chow W, Leung D, et al. J Agric Food Chem, 2012, 60: 12088
10. [10]
  [10] Suarez-Pantaleon C, Turrillas F A, Mercader J V, et al. Anal Bioanal Chem, 2012, 403: 2019
11. [11]
  [11] Liu J J, Gong P, Zhang X M, et al. Chinese Journal of Chromatography (刘靖靖, 宫萍, 张晓梅, 等. 色谱), 2012, 30(10): 1012
12. [12]
  [12] Liu J, Wang J F, Lin H, et al. Anal Chem, 2013, 85: 2847
13. [13]
  [13] Mou Y L, Guo D H, Ding Z P. Chinese Journal of Chromatography (牟艳莉, 郭德华, 丁卓平. 色谱), 2013, 31(10): 1016
14. [14]
  [14] Martinez-Hansen V, Latorre N, Royo C, et al. Cat Today, 2009, 147: S71
15. [15]
  [15] Hu Y L, Fan Y F, Li G K. J Chromatogr A, 2012, 1228: 205
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