Citation: KUANG Yuanyuan, WANG Yan, GU Xue, LI Jing, YAN Chao. Synthesis and chromatographic evaluation of sulfobetaine-based capillary zwitterionic hydrophilic monolithic column using a binary porogenic agent of polyethylene glycol/methanol[J]. Chinese Journal of Chromatography, ;2014, 32(4): 388-394. doi: 10.3724/SP.J.1123.2013.11003 shu

Synthesis and chromatographic evaluation of sulfobetaine-based capillary zwitterionic hydrophilic monolithic column using a binary porogenic agent of polyethylene glycol/methanol

1.
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China

Corresponding author: GU Xue, YAN Chao,
Received Date: 4 November 2013
Available Online: 16 December 2013
Fund Project: 国家自然科学基金项目(21175092，21105064)；国家重大科学仪器设备开发专项(2011YQ150072，2011YQ15007204，2011YQ15007207，2011YQ15007210)；上海市自然科学基金项目(12ZR1413600). (21175092，21105064)；国家重大科学仪器设备开发专项(2011YQ150072，2011YQ15007204，2011YQ15007207，2011YQ15007210)；上海市自然科学基金项目(12ZR1413600)

Sulfobetaine-based capillary zwitterionic hydrophilic monolithic columns were synthesized with a novel binary porogenic agent of polyethylene glycol (PEG)/methanol. The polymer was prepared with (3-(metharyloylamino) propyl) dimethyl (3-sulfopropyl) ammonium hydroxide inner salt (SPP) as monomer, pentaerythritol triacrylate (PETA) as crosslinker, and azobisisobutyronitrile (AIBN) as initiator. In order to optimize the properties, the contents of the polymerization mixture were investigated. The optimum preparation conditions were as follows: the mass ratio of monomer and porogenator=1:2.5; the mass ratio of SPP and PETA=1:1 in the monomer; the mass ratio of PEG and methanol=2:1 in the binary porogenic agent; the content of the initiator (AIBN) =0.1% (m/m). With the binary porogenic agent of PEG/methanol addition, good mechanical stability, homogeneous column bed, good permeability and narrow pore size distribution were obtained. In the capillary liquid chromatography mode, the hydrophilic monolith provided column efficiency up to 2.4×10⁵ plates/m which was much higher than that fabricated by traditional method without PEG/methanol. The columns were used in capillary liquid chromatography and pressurized capillary electrochromatography for the separation of a mixture of phenols, nucleosides and so on.
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沈阳化工大学材料科学与工程学院沈阳 110142