Citation: BIAN Kui, LIN Tao, LIU Min, YANG Jianwen, WANG Zongnan, HE Limin. Matrix effects in analysis of three β-agonist residues in pig edible tissues using gas chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(2): 162-168. doi: 10.3724/SP.J.1123.2013.08031 shu

Matrix effects in analysis of three β-agonist residues in pig edible tissues using gas chromatography-mass spectrometry

BIAN Kui ¹ ,
LIN Tao ² ,
LIU Min ¹ ,
YANG Jianwen ¹ ,
WANG Zongnan ¹ ,
HE Limin ^1,,

1.
1. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, South China Agriculture University, Guangzhou 510642, China;

Corresponding author: HE Limin,
Received Date: 19 August 2013
Available Online: 24 September 2013
Fund Project: 国家自然科学基金项目(31072166) (31072166)东莞市高等院校科研机构科技项目(2011108102007). (2011108102007)

A gas chromatography-mass spectrometry (GC-MS) method was established for the determination of the residues of three β-agonists (clenbuterol, salbutamol and ractopamine) in pig edible tissues. The matrix effects (MEs) in the analysis of the three compounds with the developed method were determined. The influences of matrix state and its weight on MEs were evaluated statistically. The analytes in pig liver and muscle and their corresponding freeze-dried powders were derivatized with N,O-bis(trimethylsilyl) trifluoroacetamide. Then the derivatives were determined in selected ion monitoring mode and the intensities of MEs of the three β-agonists were obtained. Significant matrix enhancement was observed for the three analytes, and especially, the ME of ractopamine was more than 1000%. The results of analysis of variance (ANOVA) demonstrated that MEs were significantly different for the three analytes in two matrices among different matrix weights (P < 0.05), and MEs of the three analytes increased from 1 g to 5 g with the increase of matrix weight. MEs for the three analytes were not significantly different between fresh pig tissues and its freeze-dried powder matrices (P > 0.05), indicating that the freeze-dried powder matrices might be used to conveniently prepare the matrix-matched calibration solution, which could efficiently compensate the MEs of the β-agonists in GC-MS analysis.
- gas chromatography-mass spectrometry (GC-MS),
- β-agonists,
- pig tissues,
- matrix effect,
- analysis of variance (ANOVA)
1. [1]
  [1] Zhai F L, Lai K Q, Zhang Y L, et al. Food Science (翟福丽, 赖克强, 张衍亮, 等. 食品科学), 2011, 32(5): 351
2. [2]
  [2] Zhang H T, Wang Z L, Deng R G, et al. Food Science (张海棠, 王自良, 邓瑞广, 等. 食品科学), 2009, 30(3): 175
3. [3]
  [3] Savant R H, Banerjee K, Utture S C, et al. J Agric Food Chem, 2010, 58(3): 1447
4. [4]
  [4] Gonzalez-Rodriguez R M, Cancho-Grande B, Simal-Gandara J. J Chromatogr A, 2009, 1216(32): 6033
5. [5]
  [5] Matuszewski B K, Constanzer M L, Chavez-Eng C M. Anal Chem, 1998, 70(5): 882
6. [6]
  [6] Poole C F. J Chromatogr A, 2007, 1158(1/2): 241
7. [7]
  [7] Wang L Z, Zhou Y, Chen Y, et al. Chinese Journal of Chromatography (王连珠, 周昱, 陈泳, 等. 色谱), 2012, 30(2): 146
8. [8]
  [8] Xu X L, Zhao H X, Li L, et al. Chinese Journal of Chromatography (许秀丽, 赵海香, 李礼, 等. 色谱), 2012, 30(3): 267
9. [9]
  [9] Yadav M, Trivedi V, Upadhyay V, et al. J Chromatogr B, 2012, 885/886: 138
10. [10]
  [10] Jiang H, Cao H, Zhang Y, et al. J Chromatogr B, 2012, 891/892: 71
11. [11]
  [11] Wang P, Jiang X H, Wang L. Chinese Journal of New Drugs (王鹏, 蒋学华, 王凌. 中国新药杂志), 2011, 20(20): 1953
12. [12]
  [12] Matuszewski B K, Constanzer M L, Chavez-Eng C M. Anal Chem, 2003, 75(13): 3019
13. [13]
  [13] Chen Y, Zhu J, Yu Z S, et al. Chinese Journal of Chromatography (陈跃, 朱军, 于忠山, 等. 色谱), 2012, 30(11): 1148
14. [14]
  [14] Lahaie M, Mess J N, Furtado M, et al. Bioanalysis, 2010, 2(6): 1011
15. [15]
  [15] Shao B, Jia X F, Zhang J, et al. Food Chem, 2009, 114(3): 1115
16. [16]
  [16] Moragues F, Igualada C. Anal Chim Acta, 2009, 637(1/2): 193
17. [17]
  [17] Wang L Q, Zeng Z L, Shu J H, et al. Chinese Journal of Analytical Chemistry (王立琦, 曾振灵, 束建花, 等. 分析化学), 2012, 40(9): 1445
18. [18]
  [18] Liu M, Liu R, Wang L Q, et al. Journal of Instrumental Analysis (刘敏, 刘戎, 王立琦, 等. 分析测试学报), 2012, 31(3): 290
19. [19]
  [19] Wu P G, Chen H H, Wang Q, et al. Chinese Journal of Chromatography (吴平谷, 陈慧华, 王强, 等. 色谱), 2008, 26(1): 39
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沈阳化工大学材料科学与工程学院沈阳 110142