Citation: ZHANG Yong, XUE Kunpeng, ZHOU An, YU Huimin, LI Yin, YAO Lixin, ZHAO Yuexing, XIE Xiaomei. Determination of triterpenoic acid isomers in Eriobotryae folium using high performance liquid chromatography on C₃₀ column[J]. Chinese Journal of Chromatography, ;2014, 32(1): 47-51. doi: 10.3724/SP.J.1123.2013.08019 shu

Determination of triterpenoic acid isomers in Eriobotryae folium using high performance liquid chromatography on C₃₀ column

ZHANG Yong ^1,, ,
XUE Kunpeng ² ,
ZHOU An ¹ ,
YU Huimin ¹ ,
LI Yin ² ,
YAO Lixin ² ,
ZHAO Yuexing ² ,
XIE Xiaomei ¹

1.
1. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230031, China;

Corresponding author: ZHANG Yong,
Received Date: 13 August 2013
Available Online: 10 September 2013
Fund Project: 安徽省教育厅自然科学基金项目(KJ2013A172) (KJ2013A172)安徽中医学院自然科学基金项目(2012zr023). (2012zr023)

A rapid and easy assay method for four triterpenoic acid isomers in Eriobotryae folium was established. The sample was extracted with methanol, then separated on a C₃₀ column (250 mm×4.6 mm, 5 μm) at 20 ℃ using acetonitrile-water (95:5, v/v) as the mobile phase at a flow rate of 1.0 mL/min. The target compounds were simultaneously detected at 210 nm, and the injection volume was 10 μ L. The validation was carried out and the resolution (R≥2.2), the precision (RSD≤1.1%), the linearity (r≥0.9992), the repeatability (RSD≤4.4%), and the recovery (range from 95.4% to 101.7%, RSD≤4.8%) were acceptable. The method is reliable for the quantification of the four triterpenoic acid isomers by HPLC on C₃₀ column.
- high performance liquid chromatography (HPLC),
- C₃₀ column,
- maslinic acid,
- corosolic acid,
- oleanolic acid,
- ursolic acid,
- isomers,
- Eriobotryae folium
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Determination of triterpenoic acid isomers in Eriobotryae folium using high performance liquid chromatography on C30 column

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Determination of triterpenoic acid isomers in Eriobotryae folium using high performance liquid chromatography on C₃₀ column