Citation: DU Jinghui, LIU Xu, XU Xiaoping. Advances in isolation and enrichment of circulating tumor cells in microfluidic chips[J]. Chinese Journal of Chromatography, ;2014, 32(1): 7-12. doi: 10.3724/SP.J.1123.2013.08009 shu

Advances in isolation and enrichment of circulating tumor cells in microfluidic chips

DU Jinghui ¹ ,
LIU Xu ^1,, ,
XU Xiaoping ^2,,

1.
1. Department of Clinical Laboratory, the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300073, China;

Corresponding author: LIU Xu, XU Xiaoping,
Received Date: 13 August 2013
Available Online: 15 November 2013
Fund Project: 国家自然科学基金项目(61106128) (61106128)广东省科技计划项目[(2011)177] (2011)深圳市科技计划项目(201101004) (201101004)深圳发展改革委员会项目[(2012)385]. (2012)

The research of circulating tumor cells (CTCs) has drawn increasing attention in recent years, because its potential value in the early diagnosis of cancers, management of clinical treatment, development of personalized medicine, and exploring the mechanism of metastasis. However, the difficulty in using CTCs lies in their extremely low concentrations. Recently, microfluidic devices have shown the potential to efficiently isolate and detect rare CTCs in cancer patients. A variety of on-chip blood analysis has been demonstrated by several groups. The advantages of microfluidics include low cost, short reaction times, high throughput, and ease of use. Recently, a variety of microfluidic devices have been developed to CTCs isolation and enrichment which can realize high capture efficiency, high purity and high throughput. In this article, we review some of the recent works in microfluidic CTCs isolation and enrichment devices and discuss in what field should be done next based on our researches.
- microfluidic devices,
- circulating tumor cells (CTCs),
- isolation,
- enrichment,
- review
1. [1]
  [1] Gupta G P, Massague J. Cell, 2006, 127(4): 679
2. [2]
  [2] Zhang P, Sun C L, Zhang R, et al. Chinese Journal of Chromatography (张鹏, 孙长龙, 张人, 等. 色谱), 2013, 31(6): 518
3. [3]
  [3] Du J H, Liu Z B, Zhang W, et al. Chinese Journal of Health Laboratory Technology (杜晶辉, 刘宗彬, 张望, 等. 中国卫生检验杂志), 2013, 23(6): 1364
4. [4]
  [4] Racila E, Euhus D, Weiss A J, et al. Proc Natl Acad Sci USA, 1998, 95(8): 4589
5. [5]
  [5] Lianidou E S, Markou A. Clin Chem, 2011, 57(9): 1242
6. [6]
  [6] Zhe X, Cher M L, Bonfil R D. Am J Cancer Res, 2011, 1(6): 740
7. [7]
  [7] Ke F, Wu J M, Yao J E, et al. Chinese Journal of Laboratory Medicine (柯峰, 吴健民, 姚见儿, 等. 中华检验医学杂志), 2007, 30(1): 77
8. [8]
  [8] Miller M C, Doyle G V, Terstappen L W. J Oncol, 2010, DOI: 10.1155/2010/617421
9. [9]
  [9] Liotta L A, Saidel M G, Kleinerman J. Cancer Res, 1976, 36(3): 889
10. [10]
  [10] Stone H A, Stroock A D, Ajdari A. Ann Rev Fluid Mech, 2004, 36: 381
11. [11]
  [11] Lin B C, Qin J H. Microfluidic Chip Laboratory. Beijing: Science Press (林炳承, 秦建华. 微流控芯片实验室. 北京: 科学出版社), 2006
12. [12]
  [12] Hughes A D, Mattison J, Powderly J D, et al. J Vis Exp, 2012, 15(64): 4248
13. [13]
  [13] Nagrath S, Sequist L V, Maheswaran S, et al. Nature, 2007, 450(7173): 1235
14. [14]
  [14] Stott S L, Hsu C H, Tsukrov D I, et al. Proc Natl Acad Sci USA, 2010, 107(43): 18392
15. [15]
  [15] Wang S, Liu K, Liu J, et al. Angew Chem Int Ed Engl, 2011, 50(13): 3084
16. [16]
  [16] Kang J H, Krause S, Tobin H, et al. Lab Chip, 2012, 12(12): 2175
17. [17]
  [17] Cristofanilli M, Budd G T, Ellis M J, et al. N Engl J Med, 2004, 351(8): 781
18. [18]
  [18] Lin M X, Hyun K A, Moon H S, et al. Biosens Bioelectron, 2013, 40(1): 63
19. [19]
  [19] Mikolajczyk S D, Millar L S, Tsinberg P, et al. J Oncol, 2011, DOI: 10.1155/2011/252361
20. [20]
  [20] Lustberg M, Jatana K R, Zborowski M, et al. Recent Results Cancer Res, 2012, 195: 97
21. [21]
  [21] Huang S B, Wu M H, Lin Y H, et al. Lab Chip, 2013, 13(7): 1371
22. [22]
  [22] Zheng S, Lin H, Liu J Q, et al. J Chromatogr A, 2007, 1162(2): 154
23. [23]
  [23] Lin H K, Zheng S, Williams A J, et al. Clin Cancer Res, 2010, 16(20): 5011
24. [24]
  [24] Liu D Y, Yan W, Zhang Q, et al. Journal of Molecular Diagnostics and Therapy (刘大渔, 严伟, 张琼, 等. 分子诊断与治疗杂志), 2012, 4(6): 366
25. [25]
  [25] Mohamed H, Murray M, Turner J N, et al. J Chromatogr A, 2009, 1216(47): 8289
26. [26]
  [26] Chung J, Shao H, Reiner T, et al. Adv Healthcare Mater, 2012, 1(4): 432
27. [27]
  [27] Park J S, Song S H, Jung H I. Lab Chip, 2009, 9(7): 939
28. [28]
  [28] Sim T S, Kwon K, Park J C, et al. Lab Chip, 2011, 11(1): 93
29. [29]
  [29] Gossett D R, Di Carlo D. Anal Chem, 2009, 81: 8459
30. [30]
  [30] Di Carlo D. Lab Chip, 2009, 9(21): 3038
31. [31]
  [31] Kuntaegowdanahalli S S, Bhagat A A, Kumar G, et al. Lab Chip, 2009, 9(20): 2973
32. [32]
  [32] Sun J, Li M, Liu C, et al. Lab Chip, 2012, 12(20): 3952
33. [33]
  [33] Inglis D W, Davis J A, Austin R H, et al. Lab Chip, 2006, 6(5): 655
34. [34]
  [34] Loutherback K, Chou K S, Newman J, et al. Microfl Nanofl, 2012, 9(6): 1143
35. [35]
  [35] Loutherback K, D'Silva J, Liu L, et al. Nat Precedings, 2012, 1: 6861
36. [36]
  [36] Liu Z B, Huang F, Du J H, et al. Biomicrofluidics, 2013, 7(1): 1801
37. [37]
  [37] Pethig R, Menachery A, Pells S, et al. J Biomed Biotechnol, 2010, DOI: 10.1155/2010/182581
38. [38]
  [38] Becker F F, Wang X B, Huang Y, et al. Proc Natl Acad Sci USA, 1995, 92(3): 860
39. [39]
  [39] Moon H S, Kwon K, Kim S I, et al. Lab Chip, 2011, 11(6): 1118
40. [40]
  [40] Kim S, Han S I, Park M J, et al. Anal Chem, 2013, 85(5): 2779
41. [41]
  [41] Augustsson P, Magnusson C, Nordin M, et al. Anal Chem, 2012, 84(18): 7954
42. [42]
  [42] Liu Z B, Zhang W, Huang F, et al. Biosens Bioelectron, 2013, 47C: 113
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