Citation: SHEN Danhong, LU Xin, CHANG Yuwei, ZHANG Junjie, ZHAO Yanni, WANG Ying, XU Guowang, ZHAO Chunxia. Determination of phenolic compounds in fresh tobacco leaves by high performance liquid chromatography-ultraviolet/mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(1): 40-46. doi: 10.3724/SP.J.1123.2013.08004 shu

Determination of phenolic compounds in fresh tobacco leaves by high performance liquid chromatography-ultraviolet/mass spectrometry

SHEN Danhong ^1,2 ,
LU Xin ¹ ,
CHANG Yuwei ¹ ,
ZHANG Junjie ¹ ,
ZHAO Yanni ¹ ,
WANG Ying ² ,
XU Guowang ¹ ,
ZHAO Chunxia ^1,,

1.
1. CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;

Corresponding author: ZHAO Chunxia,
Received Date: 1 August 2013
Available Online: 16 September 2013

Twenty-five phenolic compounds in tobacco were analyzed using high performance liquid chromatography-ultraviolet/mass spectrometry (HPLC-UV/MS), including absolute quantification of 11 main phenols by HPLC-UV and relative quantification of other 14 phenols by HPLC-MS. The validation results of this method were satisfactory. Eleven phenol standards showed good linearity with correlation coefficients (r²) of 0.9993-0.9999 over the mass concentration range from 0.90 mg/L to 99.00 mg/L (the range of chlorogenic acid and rutin was from 0.95 mg/L to 380.00 mg/L). The recoveries of the 11 phenol standards were 91.0%-112.4% with the RSDs of 0.33%-8.11% at the three spiked levels of 22.5-24.8, 45.0-49.5 and 67.5-74.3 mg/L. The reproducibility of the method was good with the RSDs of 1.48%-13.40%. In addition, the intra-day and inter-day precisions were also satisfactory with the RSDs of 0.35%-15.54%. Mature fresh tobacco leaves from Yunnan, Henan and Guizhou Provinces, China were analyzed using this method. The results showed that the total amount of main phenols was Guizhou>Yunnan>Henan and there was significant difference between Guizhou and Henan. The amount of rutin was Yunnan>Guizhou>Henan, and there were significant differences among them each other. The method is reproducible with a simple pretreatment covering a wide range of phenols, and can be applied in batch analysis of tobacco leaves.
- high performance liquid chromatography (HPLC),
- ultraviolet detection,
- mass spectrometry (MS),
- phenolic compounds,
- tobacco leaves
1. [1]
  [1] Dauwe R, Morreel K, Goeminne G, et al. Plant J, 2007, 52(2): 263
2. [2]
  [2] Yu C F, Zhang J S, Yan H Y, et al. Journal of Henan Agricultural Sciences (于存峰, 张峻松, 闫洪洋, 等. 河南农业科学), 2008(4): 10
3. [3]
  [3] Ru Q M, Wang L J, Li W M, et al. Molecules, 2012, 17(9): 11281
4. [4]
  [4] Chen Y K, Li X S, Yang G Y, et al. J Asian Nat Prod Res, 2012, 14(5): 450
5. [5]
  [5] Li Y J, Liu Y H, Wu Y P, et al. Chinese Journal of Spectro-scopy Laboratory (李应金, 刘彦红, 吴玉萍, 等. 光谱实验室), 2010, 27(6): 2396
6. [6]
  [6] Xie F W, Yu A J, Cheng Y, et al. Chromatographia, 2010, 72(11/12): 1207
7. [7]
  [7] Zhang Y F, Yang Y H, Su G S. Chemical Analysis and Meterage (张艳芳, 杨运红, 苏国岁. 化学分析计量), 2010, 19(6): 42
8. [8]
  [8] Li F J, Liu Q, Cai W S, et al. Chromatographia, 2009, 69(7/8): 743
9. [9]
  [9] Gu X G, Cai J B, Zhu X L, et al. J Sep Sci, 2005, 28(18): 2477
10. [10]
  [10] Gu X G, Tong J H. Journal of Anhui Agricultural University (谷勋刚, 童军华. 安徽农业大学学报), 2006, 33(1): 134
11. [11]
  [11] Li F J, Cai W S, Shao X G. Chinese Journal of Chromatography (李福娟, 蔡文生, 邵学广. 色谱), 2007, 25(4): 565
12. [12]
  [12] Liu F, Yang L, Sun L, et al. Acta Tabacaria Sinica (刘芳, 杨柳, 孙林, 等. 中国烟草学报), 2008, 14(6): 1
13. [13]
  [13] Zhao R F, Hu J, Li F. Modern Food Science and Technology (赵瑞峰, 胡静, 李峰. 现代食品科技), 2011, 27(8): 1036
14. [14]
  [14] Torras-Claveria L, Jáuregui O, Codina C, et al. Plant Sci, 2012, 182: 71
15. [15]
  [15] Matin-Tanguy J. Plant Growth Regul, 2001, 34(1): 135
16. [16]
  [16] Alcázar R, Marco F, Cuevas J C, et al. Biotechnol Lett, 2006, 28(23): 1867
17. [17]
  [17] Xiang G, Yang L, Zhang X, et al. Chromatographia, 2009, 70(5/6): 1007
18. [18]
  [18] Baumert A, Mock H P, Schmidt J, et al. Phytochemistry, 2001, 56(6): 535
19. [19]
  [19] Su Q, Liu Y, Guo W M, et al. Journal of Anhui Agricultural Sciences (苏强, 刘阳, 过为民, 等. 安徽农业科学), 2010, 38(24): 13083
20. [20]
  [20] Yin Y Q, Deng M J. Guangxi Tobacco (尹永强, 邓明军. 广西烟草), 2007(7): 23
1. [1]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
2. [2]
  Wei Shao , Wanqun Zhang , Pingping Zhu , Wanqun Hu , Qiang Zhou , Weiwei Li , Kaiping Yang , Xisheng Wang . Design and Practice of Ideological and Political Cases in the Course of Instrument Analysis Experiment: Taking the GC-MS Experiment as an Example. University Chemistry, 2024, 39(2): 147-154. doi: 10.3866/PKU.DXHX202309048
3. [3]
  Siming Bian , Sijie Luo , Junjie Ou . Application of van Deemter Equation in Instrumental Analysis Teaching: A New Type of Core-Shell Stationary Phase. University Chemistry, 2025, 40(3): 381-386. doi: 10.12461/PKU.DXHX202406087
4. [4]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
5. [5]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . 液相法制备硫化物固体电解质及其在全固态锂电池中的应用. Acta Physico-Chimica Sinica, 2025, 41(1): 2309019-. doi: 10.3866/PKU.WHXB202309019
6. [6]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
7. [7]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-. doi: 10.3866/PKU.WHXB202309036
8. [8]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
9. [9]
  Shengbiao Zheng , Liang Li , Nini Zhang , Ruimin Bao , Ruizhang Hu , Jing Tang . Metal-Organic Framework-Derived Materials Modified Electrode for Electrochemical Sensing of Tert-Butylhydroquinone: A Recommended Comprehensive Chemistry Experiment for Translating Research Results. University Chemistry, 2024, 39(7): 345-353. doi: 10.3866/PKU.DXHX202310096
10. [10]
  Hao Wu , Zhen Liu , Dachang Bai . ¹H NMR Spectrum of Amide Compounds. University Chemistry, 2024, 39(3): 231-238. doi: 10.3866/PKU.DXHX202309020
11. [11]
  Jiandong Liu , Zhijia Zhang , Mikhail Kamenskii , Filipp Volkov , Svetlana Eliseeva , Jianmin Ma . Research Progress on Cathode Electrolyte Interphase in High-Voltage Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(2): 100011-. doi: 10.3866/PKU.WHXB202308048
12. [12]
  Jianjun LI , Mingjie REN , Lili ZHANG , Lingling ZENG , Huiling WANG , Xiangwu MENG . UV-assisted degradation of tetracycline hydrochloride by MnFe₂O₄@activated carbon activated persulfate. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1869-1880. doi: 10.11862/CJIC.20240187
13. [13]
  Yi Li , Zhaoxiang Cao , Peng Liu , Xia Wu , Dongju Zhang . Revealing the Coloration and Color Change Mechanisms of the Eriochrome Black T Indicator through Computational Chemistry and UV-Visible Absorption Spectroscopy. University Chemistry, 2025, 40(3): 132-139. doi: 10.12461/PKU.DXHX202405154
14. [14]
  Di Yang , Jiayi Wei , Hong Zhai , Xin Wang , Taiming Sun , Haole Song , Haiyan Wang . Rapid Detection of SARS-CoV-2 Using an Innovative “Magic Strip”. University Chemistry, 2024, 39(4): 373-381. doi: 10.3866/PKU.DXHX202312023
15. [15]
  Tao Jiang , Yuting Wang , Lüjin Gao , Yi Zou , Bowen Zhu , Li Chen , Xianzeng Li . Experimental Design for the Preparation of Composite Solid Electrolytes for Application in All-Solid-State Batteries: Exploration of Comprehensive Chemistry Laboratory Teaching. University Chemistry, 2024, 39(2): 371-378. doi: 10.3866/PKU.DXHX202308057
16. [16]
  Yuhang Jiang , Weijie Liu , Jiaqi Cai , Jiayue Chen , Yanping Ren , Pingping Wu , Liulin Yang . A Journey into the Science and Art of Sugar: “Dispersion of Light and Optical Rotation of Matter” Science Popularization Experiment. University Chemistry, 2024, 39(9): 288-294. doi: 10.12461/PKU.DXHX202401054
17. [17]
  Qingyang Cui , Feng Yu , Zirun Wang , Bangkun Jin , Wanqun Hu , Wan Li . From Jelly to Soft Matter: Preparation and Properties-Exploring of Different Kinds of Hydrogels. University Chemistry, 2024, 39(9): 338-348. doi: 10.3866/PKU.DXHX202309046
18. [18]
  Zhifang SU , Zongjie GUAN , Yu FANG . Process of electrocatalytic synthesis of small molecule substances by porous framework materials. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2373-2395. doi: 10.11862/CJIC.20240290
19. [19]
  Zhuoming Liang , Ming Chen , Zhiwen Zheng , Kai Chen . Multidimensional Studies on Ketone-Enol Tautomerism of 1,3-Diketones By ¹H NMR. University Chemistry, 2024, 39(7): 361-367. doi: 10.3866/PKU.DXHX202311029
20. [20]
  Lingbang Qiu , Jiangmin Jiang , Libo Wang , Lang Bai , Fei Zhou , Gaoyu Zhou , Quanchao Zhuang , Yanhua Cui . 原位电化学阻抗谱监测长寿命热电池Nb₁₂WO₃₃正极材料的高温双放电机制. Acta Physico-Chimica Sinica, 2025, 41(5): 100040-. doi: 10.1016/j.actphy.2024.100040

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(277)
HTML views(17)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142