Citation: CHEN Dawei, MIAO Hong, ZHAO Yunfeng, WU Yongning. Effect of alcoholic strength on the determination of ethyl carbamate in Chinese spirits by high performance liquid chromatography-fluorescence detection[J]. Chinese Journal of Chromatography, ;2013, 31(12): 1206-1210. doi: 10.3724/SP.J.1123.2013.07023 shu

Effect of alcoholic strength on the determination of ethyl carbamate in Chinese spirits by high performance liquid chromatography-fluorescence detection

1.
China National Center for Food Safety Risk Assessment, Key Laboratory of Food Safety Risk Assessment, Ministry of Health, Beijing 100021, China

Corresponding author: ZHAO Yunfeng,
Received Date: 17 July 2013
Available Online: 31 July 2013
Fund Project:

A high performance liquid chromatography-fluorescence detection (HPLC-FLD) method was established for rapid determination of ethyl carbamate (EC) in Chinese spirits. Through the analysis and comparison of the EC peak areas in different alcoholic strengths determined by HPLC-FLD, the effect of alcoholic strength on the determination of the content of EC was found. The alcoholic strength and the peak area of EC showed good linearity in the range of 5% to 65% (v/v) alcohol content, and the correlation coefficients (R²) were higher than 0.98. Furthermore, the conversion between the peak area of EC with different alcoholic strengths was established by the relative correction coefficient. The method showed a good linearity in the range of 10 to 500 μg/L for EC with the average recoveries of 98.9%-108.2% and RSDs of 0.6%-4.9%. The reliability of the established HPLC-FLD method was evaluated by comparison with GC-MS method. The results showed that the results of two methods were not significantly different. The developed method is simple, sensitive, accurate, and suitable for the rapid determination of EC in Chinese spirits.
- high performance liquid chromatography (HPLC),
- ethyl carbamate (EC),
- alcoholic strength,
- Chinese spirits
1. [1]
  [1] Weber J V, Sharypov V I. Environ Chem Lett, 2009, 7(3): 233
2. [2]
  [2] Xu X, Gao Y, Cao X, et al. J Sep Sci, 2012, 35(7): 804
3. [3]
  [3] Liu J, Xu Y, Zhao G. J Inst Brew, 2012, 118(2): 217
4. [4]
  [4] Wu P, Pan X, Wang L, et al. Food Control, 2012, 23(1): 286
5. [5]
  [5] Zhou P P, Zhao Y F, Zhang G, et al. Chinese Journal of Food Hygiene (周萍萍, 赵云峰, 张珙, 等. 中国食品卫生杂志), 2007, 19(6): 492
6. [6]
  [6] Zhang X N, Ye C W, Zou G, et al. Chinese Journal of Chromatography (张雪娜, 叶长文, 邹更, 等. 色谱), 2011, 29(8): 701
7. [7]
  [7] De Melo Abreu S, Alves A, Oliveira B, et al. Anal Bioanal Chem, 2005, 382(2): 498
8. [8]
  [8] De Resende Machado A M, Cardoso M G, Saczk A A, et al. Food Chem, 2013, 138(2): 1233
9. [9]
  [9] Madrera R R, Valles B S. Food Control, 2009, 20(2): 139
10. [10]
  [10] Fu M, Liu J, Chen Q, et al. Int J Food Sci Tech, 2010, 45(6): 1297
11. [11]
  [11] Zhong Q D, Yao L, Xiong Z H. Food and Fermentation Industries (钟其顶, 姚亮, 熊正河. 食品与发酵工业), 2007, 33(3): 115
12. [12]
  [12] Gao N F, Bao J H, Sun X W, et al. China Brewing (高年发, 宝菊花, 孙晓雯, 等. 中国酿造), 2008, 27(13): 84
13. [13]
  [13] Hasnip S, Crews C, Potter N, et al. J Agric Food Chem, 2007, 55(7): 2755
14. [14]
  [14] Lachenmeier D W, Schehl B, Kuballa T, et al. Food Addit Contam, 2005, 22(5): 397
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沈阳化工大学材料科学与工程学院沈阳 110142