Citation: MA Cheng, PAN Yiting, ZHANG Qi, WANG Jifeng, QIAN Xiaohong, YING Wantao. Analysis of N-glycosylated protein and N-glycans in human plasma by hydrophilic enrichment strategy[J]. Chinese Journal of Chromatography, ;2013, 31(11): 1057-1063. doi: 10.3724/SP.J.1123.2013.05043 shu

Analysis of N-glycosylated protein and N-glycans in human plasma by hydrophilic enrichment strategy

MA Cheng ^1,2 ,
PAN Yiting ^1,2 ,
ZHANG Qi ³ ,
WANG Jifeng ² ,
QIAN Xiaohong ^1,2 ,
YING Wantao ^2,,

1.
1. School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China;

Corresponding author: YING Wantao,
Received Date: 27 May 2013
Available Online: 26 August 2013
Fund Project: 国家重点基础研究"973"项目(2011CB910603) (2011CB910603)国家高技术研究发展计划项目(2012AA020203) (2012AA020203)北京市科技新星计划项目(Z121107002512014). (Z121107002512014)

N-glycosylation of proteins is one of the most important post-translational modifications (PTM). Many diagnostic biomarkers and therapeutic targets are glycosylated proteins. However, it is still a big challenge to identify glycoproteins in human plasma, because of the high dynamic range and microheterogeneity of glycosylation. In this work, hydrophilic interaction liquid chromatography (HILIC) enrichment, high-pH reversed-phase prefractionation and high accurate mass spectrometry (MS) analysis were combined to profile the N-glycoproteins in human plasma. In total, 637 N-glycosites from 299 glycoproteins (protein groups) were identified. There were also 31 glycoforms recognized after HILIC enrichment and MS analysis. Among the results, 107 glycosylation sites (16.8%) are newly found. This study provided a simple and reliable strategy for exploring potential N-glycoprotein biomarkers from complicated biological systems.
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