Citation: ZHOU Xianjing, SHI Yanping. Determination of amino acids in honey by capillary electrophoresis with indirect ultraviolet detection[J]. Chinese Journal of Chromatography, ;2013, 31(7): 661-666. doi: 10.3724/SP.J.1123.2013.05027 shu

Determination of amino acids in honey by capillary electrophoresis with indirect ultraviolet detection

ZHOU Xianjing ^1,2 ,
SHI Yanping ^1,,

1.
1. Key Laboratory of Chemistry of Northwestern Plant Resources, CAS/Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China;

Corresponding author: SHI Yanping,
Received Date: 16 May 2013

Fund Project: 国家自然科学基金项目(21075127). (21075127)

A method of capillary electrophoresis with indirect ultraviolet (UV) detection was developed for the separation and determination of nine amino acids such as lysine, tryptophan, glutamic acid, etc. The effects of sodium dihydrogen phosphates concentration, pH of buffer and sample injection type and time on the reproducibility and efficiency were investigated. The optimum injection time was 5 s at 5 kPa. The optimum electrophoretic conditions were as follow: 10 mmol/L sodium dihydrogen phosphates (pH 10.2) containing 0.5 mmol/L cetrimonium bromide, 20 mmol/L nicotinic acid and 10%(v/v) methanol as running buffer, applied voltage of-15 kV, detection wavelength of 220 nm. The base line separation of the nine amino acids was achieved successfully within 11 min. The lowest detection limit was 0.3 mg/L. All of the nine analytes showed good linearities within 1.0-1000 mg/L. The relative standard deviations of migration time and peak area were 0.64%-5.83%. The recoveries of the eight amino acids spiked in a real sample were between 60.00% and 118.37%. The method was applied in the determination of the amino acids in honey samples from different nectar plants and origins. Prolin, serine and aspartic acid were found in five honey samples, and tryptophan was only found in a litchi honey sample. This method can provide good reference to the evaluation of the quality and nectar origin of honey.
- capillary electrophoresis (CE),
- indirect ultraviolet detection,
- amino acids,
- honey
1. [1]
  [1] Blasa M, Candiracci M, Accorsi A, et al. Food Chem, 2007, 104: 1635
2. [2]
  [2] Lachman J, Orsák M, Hejtmánková A, et al. LWT-Food Sci Technol, 2010, 43: 52
3. [3]
  [3] Boorn K L, Khor Y Y, Sweetman E, et al. J Applied Micro, 2010, 108: 1534
4. [4]
  [4] Dong R, Zheng Y N. Food Science (董蕊, 郑毅男. 食品科学), 2011, 32(21): 66http://mall.cnki.net/magazine/Article/SPKX201121014.htm
5. [5]
  [5] Cotte J F, Casabianca H, Giroud B, et al. Anal Bioanal Chem, 2004, 378(5): 1342
6. [6]
  [6] Hamide Z, Senyuva H Z, Gilbert J, et al. J Agric Food Chem, 2009, 57(9): 3911
7. [7]
  [7] Katarzyna J, Magda A, Piotr N. Pol J Food Nutr Sci, 2012, 62(2): 85
8. [8]
  [8] Sun Y C, Xu X Z, Xu Y L, et al. Chinese Journal of Chromatography (孙言春, 许宪祝, 徐衍岭, 等. 色谱), 2013, 31(3): 275
9. [9]
  [9] Feng L, Yan A P, Wan Y Q, et al. Chinese Journal of Chromatography (冯蕾, 鄢爱平, 万益群, 等. 色谱), 2010, 28(4): 408
10. [10]
  [10] Tang X X, Gu Y, Wang C, et al. Chinese Journal of Chromatography (汤新星, 顾源, 王畅, 等. 色谱), 2012, 30(7): 696
11. [11]
  [11] Wang Y H, Shen K Y, Li P, et al. Chinese Journal of Chromatography (王玉红, 申克宇, 李鹏, 等. 色谱), 2011, 29(9): 908
12. [12]
  [12] Pereira V, Pontes M, Camara J S, et al. J Chromatogr A, 2008, 1189(1/2): 435
13. [13]
  [13] Huang Y T, Chen P C, Chen R L C, et al. Food Chem, 2010, 118(3): 876
14. [14]
  [14] Hu J H, Qiu L Y, Wang C R, et al. Food Science and Technology (胡建鸿, 邱利焱, 王成润, 等. 食品科技), 2008(10): 211http://www.cnki.com.cn/Journal/B-B6-SSPJ-2008-10.htm
15. [15]
  [15] Rebane R, Herodes K. J Chromatogr A, 2012, 1245(6): 134
16. [16]
  [16] Huang H, Gong X H, Liu H H, et al. Food Science (黄会, 宫向红, 刘慧慧, 等. 食品科学), 2012, 33(8): 207http://mall.cnki.net/magazine/magadetail/SPKX201208.htm
17. [17]
  [17] Fu C G, Yang D Z, Wang L X. Food Science (傅崇岗, 杨冬芝, 王立新. 食品科学), 2005, 26(12): 196http://www.cnki.com.cn/Journal/B-B6-SPKX-2005-12.htm
18. [18]
  [18] Fu G N, He Y Z, Wang X K, et al. Chinese Journal of Chromatography (付国妮, 何友昭, 王晓葵, 等. 色谱), 2007, 25(2): 193
19. [19]
  [19] Chen B, Li X G, He P, et al. Chinese Journal of Chromatography (陈冰, 李小戈, 何萍, 等. 色谱), 2004, 22(1): 74
20. [20]
  [20] Yan H S, Cheng X H, He B L. Chemical Journal of Chinese Universities (阎虎生, 程晓辉, 何炳林. 高等学校化学学报), 1992, 13(2): 270http://www.cjcu.jlu.edu.cn/CN/abstract/abstract21109.shtml
21. [21]
  [21] Liu J X, Liu G D, Yan H S, et al. Chemical Journal of Chinese Universities (刘菊湘, 刘国栋, 阎虎生, 等. 高等学校化学学报), 2001, 22(12): 2100http://wuxizazhi.cnki.net/Article/GDXH200112037.html
1. [1]
  Hong CAI , Jiewen WU , Jingyun LI , Lixian CHEN , Siqi XIAO , Dan LI . Synthesis of a zinc-cobalt bimetallic adenine metal-organic framework for the recognition of sulfur-containing amino acids. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 114-122. doi: 10.11862/CJIC.20240382
2. [2]
  Jianjun LI , Mingjie REN , Lili ZHANG , Lingling ZENG , Huiling WANG , Xiangwu MENG . UV-assisted degradation of tetracycline hydrochloride by MnFe₂O₄@activated carbon activated persulfate. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1869-1880. doi: 10.11862/CJIC.20240187
3. [3]
  Yi Li , Zhaoxiang Cao , Peng Liu , Xia Wu , Dongju Zhang . Revealing the Coloration and Color Change Mechanisms of the Eriochrome Black T Indicator through Computational Chemistry and UV-Visible Absorption Spectroscopy. University Chemistry, 2025, 40(3): 132-139. doi: 10.12461/PKU.DXHX202405154
4. [4]
  Qingying Gao , Tao Luo , Jianyuan Su , Chaofan Yu , Jiazhu Li , Bingfei Yan , Wenzuo Li , Zhen Zhang , Yi Liu . Refinement and Expansion of the Classic Cinnamic Acid Synthesis Experiment. University Chemistry, 2024, 39(5): 243-250. doi: 10.3866/PKU.DXHX202311074
5. [5]
  Di Yang , Jiayi Wei , Hong Zhai , Xin Wang , Taiming Sun , Haole Song , Haiyan Wang . Rapid Detection of SARS-CoV-2 Using an Innovative “Magic Strip”. University Chemistry, 2024, 39(4): 373-381. doi: 10.3866/PKU.DXHX202312023
6. [6]
  Yongqing Kuang , Jie Liu , Jianjun Feng , Wen Yang , Shuanglian Cai , Ling Shi . Experimental Design for the Two-Step Synthesis of Paracetamol from 4-Hydroxyacetophenone. University Chemistry, 2024, 39(8): 331-337. doi: 10.12461/PKU.DXHX202403012
7. [7]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
8. [8]
  Meijin Li , Xirong Fu , Xue Zheng , Yuhan Liu , Bao Li . The Marvel of NAD⁺: Nicotinamide Adenine Dinucleotide. University Chemistry, 2024, 39(9): 35-39. doi: 10.12461/PKU.DXHX202401027
9. [9]
  Keying Qu , Jie Li , Ziqiu Lai , Kai Chen . Unveiling the Mystery of Chirality from Tartaric Acid. University Chemistry, 2024, 39(9): 369-378. doi: 10.12461/PKU.DXHX202310091
10. [10]
  Ran Yu , Chen Hu , Ruili Guo , Ruonan Liu , Lixing Xia , Cenyu Yang , Jianglan Shui . 杂多酸H₃PW₁₂O₄₀高效催化MgH₂储氢. Acta Physico-Chimica Sinica, 2025, 41(1): 2308032-. doi: 10.3866/PKU.WHXB202308032
11. [11]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
12. [12]
  Yang YANG , Pengcheng LI , Zhan SHU , Nengrong TU , Zonghua WANG . Plasmon-enhanced upconversion luminescence and application of molecular detection. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 877-884. doi: 10.11862/CJIC.20230440
13. [13]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
14. [14]
  Xiaowei TANG , Shiquan XIAO , Jingwen SUN , Yu ZHU , Xiaoting CHEN , Haiyan ZHANG . A zinc complex for the detection of anthrax biomarker. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1850-1860. doi: 10.11862/CJIC.20240173
15. [15]
  Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120
16. [16]
  Tongyu Zheng , Teng Li , Xiaoyu Han , Yupei Chai , Kexin Zhao , Quan Liu , Xiaohui Ji . A DIY pH Detection Agent Using Persimmon Extract for Acid-Base Discoloration Popularization Experiment. University Chemistry, 2024, 39(5): 27-36. doi: 10.3866/PKU.DXHX202309107
17. [17]
  Qin Tu , Anju Tao , Tongtong Ma , Jinyi Wang . Innovative Experimental Teaching of Escherichia coli Detection Based on Paper Chip. University Chemistry, 2024, 39(6): 271-277. doi: 10.3866/PKU.DXHX202309062
18. [18]
  Jinghan ZHANG , Guanying CHEN . Progress in the application of rare-earth-doped upconversion nanoprobes in biological detection. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2335-2355. doi: 10.11862/CJIC.20240249
19. [19]
  Yunchao Li , Shanying Chen , Ke Qi , Kangning Huo , Shuxin Li , Jingyi Li , Ying Wei , Louzhen Fan . A New Colloid Electrophoresis Experiment Incorporating Characteristics of Inquiry Learning and Ideological and Political Education. University Chemistry, 2024, 39(2): 47-51. doi: 10.3866/PKU.DXHX202308063
20. [20]
  Shanying Chen , Kangning Huo , Ke Qi , Jingyi Li , Shuxin Li , Yunchao Li . A Novel Colloid Electrophoresis Experiment with the Characteristics of Resource Recycling and Inquiry-Driven Experimental Design. University Chemistry, 2024, 39(5): 274-286. doi: 10.3866/PKU.DXHX202311067

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(179)
HTML views(8)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142