Citation: HAO Ying, WANG Rong, YIN Qiang, XIE Hua, LI Wenbin, JIA Zhengping. Analysis of difference of proteins of gastric cancer tissue and adjacent normal tissue by capillary electrophoresis with laser-induced fluorescence detection[J]. Chinese Journal of Chromatography, ;2013, 31(10): 1005-1009. doi: 10.3724/SP.J.1123.2013.04045 shu

Analysis of difference of proteins of gastric cancer tissue and adjacent normal tissue by capillary electrophoresis with laser-induced fluorescence detection

HAO Ying ^1,2 ,
WANG Rong ^1,2,, ,
YIN Qiang ¹ ,
XIE Hua ¹ ,
LI Wenbin ¹ ,
JIA Zhengping ^1,2,,

1.
1. PLA Key Laboratory of the Plateau of the Environmental Damage Control, Base of Clinic Pharmacology, Lanzhou General Hospital, Lanzhou Command, Lanzhou 730050, China;

Corresponding author: WANG Rong, JIA Zhengping,
Received Date: 25 April 2013
Available Online: 20 June 2013
Fund Project: 国家自然科学基金项目(20775089) (20775089)全军医药卫生"十一五"科研课题(06MB096). (06MB096)

Gastric cancer is one of the familiar malignant tumors in clinical study. Research on tumor biomarkers has increased noticeably in recent years. An experimental method of protein separation by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was established and improved. A series of effects on capillary electrophoresis (CE) were studied, such as the dynamic coating method of capillary, concentration of polyethylene oxide (PEO) as sieving medium, pH of running buffer, separation voltage, temperature and fluorescent dye. Then the optimized method was established for the determination of gastric cancer tissue and adjacent normal tissue. According to the analysis of the protein fingerprints, the results showed that the similarity of protein was up to 0.8. The molecular masses of differential proteins were almost between 50000 Da and 100000 Da. It indicated that these proteins might be potential biomarkers for cancer diagnosis which may reduce the searching scope. By statistical analysis of histological classification and electrophoresis peak numbers, this method was demonstrated reliably, and had the potential for clinical applications.
- capillary electrophoresis (CE),
- laser-induced fluorescence detection,
- protein,
- difference,
- gastric cancer tissue,
- adjacent normal tissue
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