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Citation: GUO Chengye, WANG Houyu, ZHANG Lei, FAN Liuyin, CAO Chengxi. Determination of total protein content in soya-bean milk via visual moving reaction boundary titration[J]. Chinese Journal of Chromatography, ;2013, 31(11): 1064-1070. doi: 10.3724/SP.J.1123.2013.04040 shu

Determination of total protein content in soya-bean milk via visual moving reaction boundary titration

GUO Chengye ^1,2 ,
WANG Houyu ² ,
ZHANG Lei ^1,, ,
FAN Liuyin ² ,
CAO Chengxi ^2,,

1.
1. College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;

Corresponding author: ZHANG Lei, CAO Chengxi,
Received Date: 23 April 2013
Available Online: 26 May 2013
Fund Project: 科技部重大仪器专项基金项目(2011YQ030139) (2011YQ030139)国家自然科学基金项目(21275099,21035004). (21275099,21035004)

A visual, rapid and accurate moving reaction boundary titration (MRBT) method was used for the determination of the total protein in soya-bean milk. During the process, moving reaction boundary (MRB) was formed by hydroxyl ions in the catholyte and soya-bean milk proteins immobilized in polyacrylamide gel (PAG), and an acid-base indicator was used to denote the boundary motion. The velocity of MRB has a relationship with protein concentration, which was used to obtain a standard curve. By paired t-test, there was no significant difference of the protein content between MRBT and Kjeldahl method at 95% confidence interval. The procedure of MRBT method required about 10 min, and it had linearity in the range of 2.0-14.0 g/L, low limit of detection (0.05 g/L), good precision (RSD of intra-day<1.90% and inter-day<4.39%), and high recoveries (97.41%-99.91%). In addition, non-protein nitrogen (NPN) such as melamine added into the soya-bean milk had weak influence on MRBT results.
- moving reaction boundary,
- electrophoresis,
- titration,
- total protein content,
- soya-bean milk
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