Citation: PAN Yiting, MA Cheng, BAI Haihong, ZHANG Yangjun, QIN Weijie, QIAN Xiaohong. Preparation of a novel hydrophilic open-tubular capillary column by surface initiated atom transfer radical polymerization and applications in capillary electrochromatography[J]. Chinese Journal of Chromatography, ;2013, 31(10): 995-1000. doi: 10.3724/SP.J.1123.2013.03055 shu

Preparation of a novel hydrophilic open-tubular capillary column by surface initiated atom transfer radical polymerization and applications in capillary electrochromatography

PAN Yiting ^1,2 ,
MA Cheng ^1,2 ,
BAI Haihong ^1,2 ,
ZHANG Yangjun ² ,
QIN Weijie ^2,, ,
QIAN Xiaohong ^2,,

1.
1. School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China;

Corresponding author: QIN Weijie, QIAN Xiaohong,
Received Date: 29 March 2013
Available Online: 24 April 2013
Fund Project: 国家重大科学计划项目(2013CB911204) (2013CB911204)国家重大科学仪器设备开发专项项目(2011YQ09000504) (2011YQ09000504)国家自然科学基金项目(21275005,21235001). (21275005,21235001)

A novel type of glycopolymer brushes grafted open-tubular capillary column (OTCC) was developed for the polar compound separation. Briefly, the glycerol methacrylate (GMA) polymer brushes were grafted on the inner wall of OTCC by the surface initiated atom transfer radical polymerization (SI-ATRP). Next, glucose was coupled to the side chains of the GMA polymer brushes to obtain the hydrophilic stationary phase (PGMA-N-Glucose). The optimized SI-ATRP reaction conditions were GMA/CuCl/CuCl₂/cyclohexanol system grafting at 25 ℃ for 1 h. After the glucose coupling, the column back pressure was about 3585 kPa. The structure of the glycopolymer brushes on the inner surface of OTCC was characterized by scanning electron microscopy (SEM). The glycopolymer grafting resulted in the formation of three-dimensional wave-like polymer structure on the inner surface of OTCC and largely increased the interior surface area. Therefore, improved column efficiency and loading capacity can be achieved. Under the optimized conditions, the electro osmotic flow (EOF) strength of the glycopolymer brushes grafted OTCC was obviously less than that of the bare column when the pH value of the mobile phase ranging from 3 to 11. Using the glycopolymer brushes grafted OTCC, the baseline separations of polar molecules and proteins were obtained without peak tailing. The future work will focus on the further development of the glycopolymer brushes for the highly polar compound separation, such as glycans and glycoproteins.
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沈阳化工大学材料科学与工程学院沈阳 110142

Preparation of a novel hydrophilic open-tubular capillary column by surface initiated atom transfer radical polymerization and applications in capillary electrochromatography

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

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通讯作者: 陈斌, bchen63@163.com