Citation: LIN Hui, XU Chunxiang, YAN Chunrong, ZHANG Zheng, WANG Suilou. Determination of congo red in beef by high performance liquid chromatography-tandem quadrupole time of flight mass spectrometry and ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(9): 914-919. doi: 10.3724/SP.J.1123.2013.03043 shu

Determination of congo red in beef by high performance liquid chromatography-tandem quadrupole time of flight mass spectrometry and ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry

LIN Hui ^1,2 ,
XU Chunxiang ^1,, ,
YAN Chunrong ¹ ,
ZHANG Zheng ¹ ,
WANG Suilou ^2,,

1.
1. Jiangsu Product Quality Testing and Inspection Institute, Nanjing 210007, China;

Corresponding author: XU Chunxiang, WANG Suilou,
Received Date: 22 March 2013
Available Online: 24 April 2013
Fund Project: 江苏省质量技术监督局科技项目(KJ112501). (KJ112501)

A method was developed for the determination of congo red in beef. The analyte was identified by high performance liquid chromatography-tandem quadrupole time of flight mass spectrometry (LC-QTOF MS) and quantitatively determined by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry. After purified by liquid-liquid extraction, the congo red in the beef sample was separated on an Agilent ZORBAX Eclipse Plus C₁₈ Rapid Resolution HD UPLC column (50 mm×2.1 mm, 1.8 μm) HPLC, using 95% (volume percentage) methanol as the mobile phase at 0.2 mL/min. The detection was performed on an AB 4000⁺ triple quadrupole mass spectrometer utilizing electrospray ionization (ESI) interface operated in negative ion mode and multiple-reaction monitoring (MRM) mode. The results showed that the linear range of congo red mass concentration was 0.03-1 mg/L with the correlation coefficient of 0.9998. The method had a good precision with the RSDs lower than 5% and the recoveries ranging from 88% to 91%. The limit of detection (LOD) of congo red was 0.01 mg/L. With good reproducibility, the method is simple, fast and effective for the determination of the illegally added congo red in beef and other meat products.
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